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Cryptosporulation in Kurthia spp. forces a rethinking of asporogenesis in Firmicutes.

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Title: Cryptosporulation in Kurthia spp. forces a rethinking of asporogenesis in Firmicutes.
Authors: Fatton, Mathilda1 (AUTHOR), Filippidou, Sevasti1,2 (AUTHOR), Junier, Thomas1,3 (AUTHOR), Cailleau, Guillaume1 (AUTHOR), Berge, Matthieu4 (AUTHOR), Poppleton, Daniel5 (AUTHOR), Blum, Thorsten B.6 (AUTHOR), Kaminek, Marek7 (AUTHOR), Odriozola, Adolfo7 (AUTHOR), Blom, Jochen8 (AUTHOR), Johnson, Shannon L.9 (AUTHOR), Abrahams, Jan Pieter6,10,11 (AUTHOR), Chain, Patrick S.7 (AUTHOR), Gribaldo, Simonetta5 (AUTHOR), Tocheva, Elitza I.12 (AUTHOR), Zuber, Benoît7 (AUTHOR), Viollier, Patrick H.4 (AUTHOR), Junier, Pilar1 (AUTHOR) pilar.junier@unine.ch
Source: Environmental Microbiology. Dec2022, Vol. 24 Issue 12, p6320-6335. 16p.
Subjects: Stem cells, Cell anatomy, Bacterial spores, Comparative genomics, Phenotypes, Magnetotellurics
Abstract: Endosporulation is a complex morphophysiological process resulting in a more resistant cellular structure that is produced within the mother cell and is called endospore. Endosporulation evolved in the common ancestor of Firmicutes, but it is lost in descendant lineages classified as asporogenic. While Kurthia spp. is considered to comprise only asporogenic species, we show here that strain 11kri321, which was isolated from an oligotrophic geothermal reservoir, produces phase‐bright spore‐like structures. Phylogenomics of strain 11kri321 and other Kurthia strains reveals little similarity to genetic determinants of sporulation known from endosporulating Bacilli. However, morphological hallmarks of endosporulation were observed in two of the four Kurthia strains tested, resulting in spore‐like structures (cryptospores). In contrast to classic endospores, these cryptospores did not protect against heat or UV damage and successive sub‐culturing led to the loss of the cryptosporulating phenotype. Our findings imply that a cryptosporulation phenotype may have been prevalent and subsequently lost by laboratory culturing in other Firmicutes currently considered as asporogenic. Cryptosporulation might thus represent an ancestral but unstable and adaptive developmental state in Firmicutes that is under selection under harsh environmental conditions. [ABSTRACT FROM AUTHOR]
Copyright of Environmental Microbiology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Cryptosporulation in Kurthia spp. forces a rethinking of asporogenesis in Firmicutes.
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  Data: <searchLink fieldCode="AR" term="%22Fatton%2C+Mathilda%22">Fatton, Mathilda</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Filippidou%2C+Sevasti%22">Filippidou, Sevasti</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Junier%2C+Thomas%22">Junier, Thomas</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Cailleau%2C+Guillaume%22">Cailleau, Guillaume</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Berge%2C+Matthieu%22">Berge, Matthieu</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Poppleton%2C+Daniel%22">Poppleton, Daniel</searchLink><relatesTo>5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Blum%2C+Thorsten+B%2E%22">Blum, Thorsten B.</searchLink><relatesTo>6</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kaminek%2C+Marek%22">Kaminek, Marek</searchLink><relatesTo>7</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Odriozola%2C+Adolfo%22">Odriozola, Adolfo</searchLink><relatesTo>7</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Blom%2C+Jochen%22">Blom, Jochen</searchLink><relatesTo>8</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Johnson%2C+Shannon+L%2E%22">Johnson, Shannon L.</searchLink><relatesTo>9</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Abrahams%2C+Jan+Pieter%22">Abrahams, Jan Pieter</searchLink><relatesTo>6,10,11</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chain%2C+Patrick+S%2E%22">Chain, Patrick S.</searchLink><relatesTo>7</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Gribaldo%2C+Simonetta%22">Gribaldo, Simonetta</searchLink><relatesTo>5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tocheva%2C+Elitza+I%2E%22">Tocheva, Elitza I.</searchLink><relatesTo>12</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zuber%2C+Benoît%22">Zuber, Benoît</searchLink><relatesTo>7</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Viollier%2C+Patrick+H%2E%22">Viollier, Patrick H.</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Junier%2C+Pilar%22">Junier, Pilar</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> pilar.junier@unine.ch</i>
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  Data: <searchLink fieldCode="JN" term="%22Environmental+Microbiology%22">Environmental Microbiology</searchLink>. Dec2022, Vol. 24 Issue 12, p6320-6335. 16p.
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  Data: <searchLink fieldCode="DE" term="%22Stem+cells%22">Stem cells</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+anatomy%22">Cell anatomy</searchLink><br /><searchLink fieldCode="DE" term="%22Bacterial+spores%22">Bacterial spores</searchLink><br /><searchLink fieldCode="DE" term="%22Comparative+genomics%22">Comparative genomics</searchLink><br /><searchLink fieldCode="DE" term="%22Phenotypes%22">Phenotypes</searchLink><br /><searchLink fieldCode="DE" term="%22Magnetotellurics%22">Magnetotellurics</searchLink>
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  Data: Endosporulation is a complex morphophysiological process resulting in a more resistant cellular structure that is produced within the mother cell and is called endospore. Endosporulation evolved in the common ancestor of Firmicutes, but it is lost in descendant lineages classified as asporogenic. While Kurthia spp. is considered to comprise only asporogenic species, we show here that strain 11kri321, which was isolated from an oligotrophic geothermal reservoir, produces phase‐bright spore‐like structures. Phylogenomics of strain 11kri321 and other Kurthia strains reveals little similarity to genetic determinants of sporulation known from endosporulating Bacilli. However, morphological hallmarks of endosporulation were observed in two of the four Kurthia strains tested, resulting in spore‐like structures (cryptospores). In contrast to classic endospores, these cryptospores did not protect against heat or UV damage and successive sub‐culturing led to the loss of the cryptosporulating phenotype. Our findings imply that a cryptosporulation phenotype may have been prevalent and subsequently lost by laboratory culturing in other Firmicutes currently considered as asporogenic. Cryptosporulation might thus represent an ancestral but unstable and adaptive developmental state in Firmicutes that is under selection under harsh environmental conditions. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Environmental Microbiology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1111/1462-2920.16145
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      – SubjectFull: Bacterial spores
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