Construction of BAC and BIBAC libraries and their applications for generation of SSR markers for genome analysis of chickpea,Cicer arietinumL.
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| Title: | Construction of BAC and BIBAC libraries and their applications for generation of SSR markers for genome analysis of chickpea,Cicer arietinumL. |
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| Authors: | Lichtenzveig, J.1,2, Scheuring, C.3, Dodge, J.3, Abbo, S.4 abbo@agri.huji.ac.il, Zhang, H.-B.3 hbz7049@tamu.edu |
| Source: | Theoretical & Applied Genetics. Feb2005, Vol. 110 Issue 3, p492-510. 19p. |
| Subjects: | Libraries, Genomes, Nucleic acids, Cell nuclei, Molecular genetics, Genetic research |
| Abstract: | Large-insert bacterial artificial chromosome (BAC) libraries, plant-transformation-competent binary BAC (BIBAC) libraries, and simple sequence repeat (SSR) markers are essential for many aspects of genomics research. We constructed a BAC library and a BIBAC library from the nuclear DNA of chickpea,Cicer arietinumL., cv. Hadas, partially digested withHindIII andBamHI, respectively. The BAC library has 14,976 clones, with an average insert size of 121 kb, and the BIBAC library consists of 23,040 clones, with an average insert size of 145 kb. The combined libraries collectively cover ca. 7.0× genomes of chickpea. We screened the BAC library with eight synthetic SSR oligos, (GA)10, (GAA)7, (AT)10, (TAA)7, (TGA)7, (CA)10, (CAA)7, and (CCA)7. Positive BACs were selected, subcloned, and sequenced for SSR marker development. Two hundred and thirty-three new chickpea SSR markers were developed and characterized by PCR, using chickpea DNA as template. These results have demonstrated that BACs are an excellent source for SSR marker development in chickpea. We also estimated the distribution of the SSR loci in the chickpea genome. The SSR motifs (TAA) n and (GA) n were much more abundant than the others, and the distribution of the SSR loci appeared non-random. The BAC and BIBAC libraries and new SSR markers will provide valuable resources for chickpea genomics research and breeding (the libraries and their filters are available to the public at). [ABSTRACT FROM AUTHOR] |
| Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 16100687 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Construction of BAC and BIBAC libraries and their applications for generation of SSR markers for genome analysis of chickpea,Cicer arietinumL. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Lichtenzveig%2C+J%2E%22">Lichtenzveig, J.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Scheuring%2C+C%2E%22">Scheuring, C.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Dodge%2C+J%2E%22">Dodge, J.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Abbo%2C+S%2E%22">Abbo, S.</searchLink><relatesTo>4</relatesTo><i> abbo@agri.huji.ac.il</i><br /><searchLink fieldCode="AR" term="%22Zhang%2C+H%2E-B%2E%22">Zhang, H.-B.</searchLink><relatesTo>3</relatesTo><i> hbz7049@tamu.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Theoretical+%26+Applied+Genetics%22">Theoretical & Applied Genetics</searchLink>. Feb2005, Vol. 110 Issue 3, p492-510. 19p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Libraries%22">Libraries</searchLink><br /><searchLink fieldCode="DE" term="%22Genomes%22">Genomes</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleic+acids%22">Nucleic acids</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+nuclei%22">Cell nuclei</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+genetics%22">Molecular genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+research%22">Genetic research</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Large-insert bacterial artificial chromosome (BAC) libraries, plant-transformation-competent binary BAC (BIBAC) libraries, and simple sequence repeat (SSR) markers are essential for many aspects of genomics research. We constructed a BAC library and a BIBAC library from the nuclear DNA of chickpea,Cicer arietinumL., cv. Hadas, partially digested withHindIII andBamHI, respectively. The BAC library has 14,976 clones, with an average insert size of 121 kb, and the BIBAC library consists of 23,040 clones, with an average insert size of 145 kb. The combined libraries collectively cover ca. 7.0× genomes of chickpea. We screened the BAC library with eight synthetic SSR oligos, (GA)10, (GAA)7, (AT)10, (TAA)7, (TGA)7, (CA)10, (CAA)7, and (CCA)7. Positive BACs were selected, subcloned, and sequenced for SSR marker development. Two hundred and thirty-three new chickpea SSR markers were developed and characterized by PCR, using chickpea DNA as template. These results have demonstrated that BACs are an excellent source for SSR marker development in chickpea. We also estimated the distribution of the SSR loci in the chickpea genome. The SSR motifs (TAA) n and (GA) n were much more abundant than the others, and the distribution of the SSR loci appeared non-random. The BAC and BIBAC libraries and new SSR markers will provide valuable resources for chickpea genomics research and breeding (the libraries and their filters are available to the public at). [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00122-004-1857-8 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 19 StartPage: 492 Subjects: – SubjectFull: Libraries Type: general – SubjectFull: Genomes Type: general – SubjectFull: Nucleic acids Type: general – SubjectFull: Cell nuclei Type: general – SubjectFull: Molecular genetics Type: general – SubjectFull: Genetic research Type: general Titles: – TitleFull: Construction of BAC and BIBAC libraries and their applications for generation of SSR markers for genome analysis of chickpea,Cicer arietinumL. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Lichtenzveig, J. – PersonEntity: Name: NameFull: Scheuring, C. – PersonEntity: Name: NameFull: Dodge, J. – PersonEntity: Name: NameFull: Abbo, S. – PersonEntity: Name: NameFull: Zhang, H.-B. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 02 Text: Feb2005 Type: published Y: 2005 Identifiers: – Type: issn-print Value: 00405752 Numbering: – Type: volume Value: 110 – Type: issue Value: 3 Titles: – TitleFull: Theoretical & Applied Genetics Type: main |
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