Effects of sodium chloride addition on immunoglobulin G partitioning and solubility in polyethylene glycol/hydroxypropyl starch aqueous two-phase system.
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| Title: | Effects of sodium chloride addition on immunoglobulin G partitioning and solubility in polyethylene glycol/hydroxypropyl starch aqueous two-phase system. |
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| Authors: | Wu, Qiang1,2 (AUTHOR) zqwu66@163.com, Li, Shi-Rong1 (AUTHOR), Wang, Yu-Hui1 (AUTHOR), Wang, Hong-Gang1 (AUTHOR), Wang, Zhen-Ming1 (AUTHOR), Li, Min1 (AUTHOR), Lin, Dong-Qiang1,2 (AUTHOR) lindq@zju.edu.cn |
| Source: | Process Biochemistry. Nov2022:Part 1, Vol. 122, p299-305. 7p. |
| Subjects: | Immunoglobulin G, Polyethylene glycol, Isothermal titration calorimetry, Hydrophobic interactions |
| Abstract: | The effect mechanisms of sodium chloride (NaCl) addition on the partitioning and solubility of immunoglobulin G (IgG) in polyethylene glycol (PEG)/hydroxypropyl starch (HPS) aqueous two-phase system (ATPS) were studied. The influences of NaCl on PEG/HPS phase diagram and the hydrophobicity of IgG were investigated. The results indicated that the hydrophobicity difference between two phases and the IgG hydrophobicity were both enhanced with NaCl addition, so IgG preferred to partition into the top phase with more hydrophobicity. The solubilities of IgG in PEG and HPS solutions with or without NaCl were measured. It was found that NaCl addition could significantly reduce IgG precipitation in PEG solution. The effects of NaCl on the interaction between IgG and PEG was studied by Isothermal Titration Calorimetry (ITC). It was found that the main interactions between IgG and PEG changed from electrostatic force to hydrophobic interaction with NaCl addition. IgG would be wrapped by PEG molecules due to hydrophobic interaction so that the solubility of IgG in PEG solution was enhanced. The results reveal the mechanisms of NaCl addition on the partitioning and solubility of IgG in PEG/HPS systems, and PEG/HPS ATPS might be a promising alternative for IgG separation with good purity and yield. [Display omitted] • Mechanisms of NaCl on partition and solubility of IgG in PEG/HPS system were revealed. • Hydrophobicity of IgG with NaCl addition was determined by fluorescence probe method. • Solubility of IgG in PEG solution was significantly improved with NaCl addition. • Interactions between IgG and PEG were measured by Isothermal Titration Calorimetry. [ABSTRACT FROM AUTHOR] |
| Copyright of Process Biochemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 161015869 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Effects of sodium chloride addition on immunoglobulin G partitioning and solubility in polyethylene glycol/hydroxypropyl starch aqueous two-phase system. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Wu%2C+Qiang%22">Wu, Qiang</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> zqwu66@163.com</i><br /><searchLink fieldCode="AR" term="%22Li%2C+Shi-Rong%22">Li, Shi-Rong</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Yu-Hui%22">Wang, Yu-Hui</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Hong-Gang%22">Wang, Hong-Gang</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Zhen-Ming%22">Wang, Zhen-Ming</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Li%2C+Min%22">Li, Min</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lin%2C+Dong-Qiang%22">Lin, Dong-Qiang</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> lindq@zju.edu.cn</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Process+Biochemistry%22">Process Biochemistry</searchLink>. Nov2022:Part 1, Vol. 122, p299-305. 7p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Immunoglobulin+G%22">Immunoglobulin G</searchLink><br /><searchLink fieldCode="DE" term="%22Polyethylene+glycol%22">Polyethylene glycol</searchLink><br /><searchLink fieldCode="DE" term="%22Isothermal+titration+calorimetry%22">Isothermal titration calorimetry</searchLink><br /><searchLink fieldCode="DE" term="%22Hydrophobic+interactions%22">Hydrophobic interactions</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The effect mechanisms of sodium chloride (NaCl) addition on the partitioning and solubility of immunoglobulin G (IgG) in polyethylene glycol (PEG)/hydroxypropyl starch (HPS) aqueous two-phase system (ATPS) were studied. The influences of NaCl on PEG/HPS phase diagram and the hydrophobicity of IgG were investigated. The results indicated that the hydrophobicity difference between two phases and the IgG hydrophobicity were both enhanced with NaCl addition, so IgG preferred to partition into the top phase with more hydrophobicity. The solubilities of IgG in PEG and HPS solutions with or without NaCl were measured. It was found that NaCl addition could significantly reduce IgG precipitation in PEG solution. The effects of NaCl on the interaction between IgG and PEG was studied by Isothermal Titration Calorimetry (ITC). It was found that the main interactions between IgG and PEG changed from electrostatic force to hydrophobic interaction with NaCl addition. IgG would be wrapped by PEG molecules due to hydrophobic interaction so that the solubility of IgG in PEG solution was enhanced. The results reveal the mechanisms of NaCl addition on the partitioning and solubility of IgG in PEG/HPS systems, and PEG/HPS ATPS might be a promising alternative for IgG separation with good purity and yield. [Display omitted] • Mechanisms of NaCl on partition and solubility of IgG in PEG/HPS system were revealed. • Hydrophobicity of IgG with NaCl addition was determined by fluorescence probe method. • Solubility of IgG in PEG solution was significantly improved with NaCl addition. • Interactions between IgG and PEG were measured by Isothermal Titration Calorimetry. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Process Biochemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.procbio.2022.09.009 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 7 StartPage: 299 Subjects: – SubjectFull: Immunoglobulin G Type: general – SubjectFull: Polyethylene glycol Type: general – SubjectFull: Isothermal titration calorimetry Type: general – SubjectFull: Hydrophobic interactions Type: general Titles: – TitleFull: Effects of sodium chloride addition on immunoglobulin G partitioning and solubility in polyethylene glycol/hydroxypropyl starch aqueous two-phase system. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Wu, Qiang – PersonEntity: Name: NameFull: Li, Shi-Rong – PersonEntity: Name: NameFull: Wang, Yu-Hui – PersonEntity: Name: NameFull: Wang, Hong-Gang – PersonEntity: Name: NameFull: Wang, Zhen-Ming – PersonEntity: Name: NameFull: Li, Min – PersonEntity: Name: NameFull: Lin, Dong-Qiang IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 11 Text: Nov2022:Part 1 Type: published Y: 2022 Identifiers: – Type: issn-print Value: 13595113 Numbering: – Type: volume Value: 122 Titles: – TitleFull: Process Biochemistry Type: main |
| ResultId | 1 |
