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Ultrastructure of human ovarian tissues and risk of cancer cells re-implantation after transplantation to chick embryo chorioallantois membrane (CAM) following vitrification or slow freezing.

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Title: Ultrastructure of human ovarian tissues and risk of cancer cells re-implantation after transplantation to chick embryo chorioallantois membrane (CAM) following vitrification or slow freezing.
Authors: Vatanparast, Mahboubeh1 (AUTHOR), Karimizarchi, Mojgan2 (AUTHOR), Halvaei, Iman3 (AUTHOR), Grazia Palmerini, Maria4 (AUTHOR), Macchiarelli, Guido4 (AUTHOR), Khalili, Mohammad Ali1,5 (AUTHOR) Khalili59@hotmail.com
Source: Cryobiology. Mar2023, Vol. 110, p93-102. 10p.
Subjects: Frozen human embryos, Chicken embryos, Embryo transfer, Vitrification, Chorioallantois, Disease risk factors
Abstract: Ovarian follicle depletion and premature ovarian failure are significant challenges in cancer patients subjected to radio- or chemotherapy. Ovarian tissue (OT) cryopreservation would be an option when other fertility preservation methods are not accessible. This study aimed to analyze the structure and ultrastructure of human OTs transplanted onto chick embryo chorioallantois membrane (CAM) after cryopreservation by vitrification or slow freezing. OTs from 10 cancer patients underwent cryopreservation. CAM transplantation was done on fresh and cryopreserved OTs, to assign samples to nine study groups as follows: 1) FI-FIII = fresh, 5- and 10-days post-CAM transplantation groups; 2) VI-VIII = vitrified, 5- and 10-days post-transplantation vitrified groups; 3) SFI-SFIII: slow frozen, 5- and 10-days post-transplantation slow freezing groups. Proliferation ability, folliculogenesis, and structural and ultrastructure were analyzed. The density of primordial follicles did not change after both freezing methods, but reduced after 5 (P ≥ 0.05) and 10 days (P ≤ 0.05) post-CAM transplantation. The follicular grade significantly decreased in all transplanted tissues (P ≤ 0.0). The proliferation marker increased after cryopreservation, but reduced after transplantation (P ≤ 0.05). TEM evaluation showed better follicular ultrastructure in the fresh group, after transplantation. Stromal ultrastructure appeared more preserved after vitrification compared with slow freezing. There was no sign of malignant cell contamination after transplantation. Some follicular TEM abnormalities were found in both methods of freezing, with a better transplantation rate after vitrification. Also, enhanced follicular activation resulted in faster follicular depletion in this method. The information regarding post grafting events would improve our knowledge for longer OTs' lifespans. [ABSTRACT FROM AUTHOR]
Copyright of Cryobiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Label: Title
  Group: Ti
  Data: Ultrastructure of human ovarian tissues and risk of cancer cells re-implantation after transplantation to chick embryo chorioallantois membrane (CAM) following vitrification or slow freezing.
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  Label: Authors
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  Data: <searchLink fieldCode="AR" term="%22Vatanparast%2C+Mahboubeh%22">Vatanparast, Mahboubeh</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Karimizarchi%2C+Mojgan%22">Karimizarchi, Mojgan</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Halvaei%2C+Iman%22">Halvaei, Iman</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Grazia+Palmerini%2C+Maria%22">Grazia Palmerini, Maria</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Macchiarelli%2C+Guido%22">Macchiarelli, Guido</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Khalili%2C+Mohammad+Ali%22">Khalili, Mohammad Ali</searchLink><relatesTo>1,5</relatesTo> (AUTHOR)<i> Khalili59@hotmail.com</i>
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  Data: <searchLink fieldCode="JN" term="%22Cryobiology%22">Cryobiology</searchLink>. Mar2023, Vol. 110, p93-102. 10p.
– Name: Subject
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  Data: <searchLink fieldCode="DE" term="%22Frozen+human+embryos%22">Frozen human embryos</searchLink><br /><searchLink fieldCode="DE" term="%22Chicken+embryos%22">Chicken embryos</searchLink><br /><searchLink fieldCode="DE" term="%22Embryo+transfer%22">Embryo transfer</searchLink><br /><searchLink fieldCode="DE" term="%22Vitrification%22">Vitrification</searchLink><br /><searchLink fieldCode="DE" term="%22Chorioallantois%22">Chorioallantois</searchLink><br /><searchLink fieldCode="DE" term="%22Disease+risk+factors%22">Disease risk factors</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Ovarian follicle depletion and premature ovarian failure are significant challenges in cancer patients subjected to radio- or chemotherapy. Ovarian tissue (OT) cryopreservation would be an option when other fertility preservation methods are not accessible. This study aimed to analyze the structure and ultrastructure of human OTs transplanted onto chick embryo chorioallantois membrane (CAM) after cryopreservation by vitrification or slow freezing. OTs from 10 cancer patients underwent cryopreservation. CAM transplantation was done on fresh and cryopreserved OTs, to assign samples to nine study groups as follows: 1) FI-FIII = fresh, 5- and 10-days post-CAM transplantation groups; 2) VI-VIII = vitrified, 5- and 10-days post-transplantation vitrified groups; 3) SFI-SFIII: slow frozen, 5- and 10-days post-transplantation slow freezing groups. Proliferation ability, folliculogenesis, and structural and ultrastructure were analyzed. The density of primordial follicles did not change after both freezing methods, but reduced after 5 (P ≥ 0.05) and 10 days (P ≤ 0.05) post-CAM transplantation. The follicular grade significantly decreased in all transplanted tissues (P ≤ 0.0). The proliferation marker increased after cryopreservation, but reduced after transplantation (P ≤ 0.05). TEM evaluation showed better follicular ultrastructure in the fresh group, after transplantation. Stromal ultrastructure appeared more preserved after vitrification compared with slow freezing. There was no sign of malignant cell contamination after transplantation. Some follicular TEM abnormalities were found in both methods of freezing, with a better transplantation rate after vitrification. Also, enhanced follicular activation resulted in faster follicular depletion in this method. The information regarding post grafting events would improve our knowledge for longer OTs' lifespans. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Cryobiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.cryobiol.2022.11.240
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 10
        StartPage: 93
    Subjects:
      – SubjectFull: Frozen human embryos
        Type: general
      – SubjectFull: Chicken embryos
        Type: general
      – SubjectFull: Embryo transfer
        Type: general
      – SubjectFull: Vitrification
        Type: general
      – SubjectFull: Chorioallantois
        Type: general
      – SubjectFull: Disease risk factors
        Type: general
    Titles:
      – TitleFull: Ultrastructure of human ovarian tissues and risk of cancer cells re-implantation after transplantation to chick embryo chorioallantois membrane (CAM) following vitrification or slow freezing.
        Type: main
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            NameFull: Vatanparast, Mahboubeh
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            NameFull: Karimizarchi, Mojgan
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            NameFull: Halvaei, Iman
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            NameFull: Grazia Palmerini, Maria
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            NameFull: Macchiarelli, Guido
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            NameFull: Khalili, Mohammad Ali
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          Dates:
            – D: 01
              M: 03
              Text: Mar2023
              Type: published
              Y: 2023
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              Value: 110
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            – TitleFull: Cryobiology
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