View in EDS

Using pressure-driven flow systems to evaluate laser speckle contrast imaging.

Saved in:
Bibliographic Details
Title: Using pressure-driven flow systems to evaluate laser speckle contrast imaging.
Authors: Sullender, Colin T.1 (AUTHOR) csullender@utexas.edu, Santorelli, Adam1 (AUTHOR) adam.santorelli@austin.utexas.edu, Richards, Lisa M.1 (AUTHOR) lisa.richards@utexas.edu, Mannava, Pawan K.1 (AUTHOR) mannava.pawan@utexas.edu, Smith, Christopher1 (AUTHOR) christopher.smith@utexas.edu, Dunn, Andrew K.1 (AUTHOR) adunn@mail.utexas.edu
Source: Journal of Biomedical Optics. Mar2023, Vol. 28 Issue 3, p36003-36003. 1p.
Subjects: Speckle interference, Speckle interferometry, Flow sensors, Flow measurement, Microfluidic devices
Abstract: Microfluidic flow phantom studies are commonly used for characterizing the performance of laser speckle contrast imaging (LSCI) instruments. The selection of the flow control system is critical for the reliable generation of flow during testing. The majority of recent LSCI studies using microfluidics used syringe pumps for flow control. We quantified the uncertainty in flow generation for a syringe pump and a pressure-regulated flow system. We then assessed the performance of both LSCI and multi-exposure speckle imaging (MESI) using the pressure-regulated flow system across a range of flow speeds. The syringe pump and pressure-regulated flow systems were evaluated during stepped flow profile experiments in a microfluidic device using an inline flow sensor. The uncertainty associated with each flow system was calculated and used to determine the reliability for instrument testing. The pressure-regulated flow system was then used to characterize the relative performance of LSCI and MESI during stepped flow profile experiments while using the inline flow sensor as reference. The pressure-regulated flow system produced much more stable and reproducible flow outputs compared to the syringe pump. The expanded uncertainty for the syringe pump was 8 to 20 × higher than that of the pressure-regulated flow system across the tested flow speeds. Using the pressure-regulated flow system, MESI outperformed single-exposure LSCI at all flow speeds and closely mirrored the flow sensor measurements, with average errors of 4.6 % ± 2.6 % and 15.7 % ± 4.6 % , respectively. Pressure-regulated flow systems should be used instead of syringe pumps when assessing the performance of flow measurement techniques with microfluidic studies. MESI offers more accurate relative flow measurements than traditional LSCI across a wide range of flow speeds. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Biomedical Optics is the property of SPIE - International Society of Optical Engineering and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 162800882
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Using pressure-driven flow systems to evaluate laser speckle contrast imaging.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Sullender%2C+Colin+T%2E%22">Sullender, Colin T.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> csullender@utexas.edu</i><br /><searchLink fieldCode="AR" term="%22Santorelli%2C+Adam%22">Santorelli, Adam</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> adam.santorelli@austin.utexas.edu</i><br /><searchLink fieldCode="AR" term="%22Richards%2C+Lisa+M%2E%22">Richards, Lisa M.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> lisa.richards@utexas.edu</i><br /><searchLink fieldCode="AR" term="%22Mannava%2C+Pawan+K%2E%22">Mannava, Pawan K.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> mannava.pawan@utexas.edu</i><br /><searchLink fieldCode="AR" term="%22Smith%2C+Christopher%22">Smith, Christopher</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> christopher.smith@utexas.edu</i><br /><searchLink fieldCode="AR" term="%22Dunn%2C+Andrew+K%2E%22">Dunn, Andrew K.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> adunn@mail.utexas.edu</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Journal+of+Biomedical+Optics%22">Journal of Biomedical Optics</searchLink>. Mar2023, Vol. 28 Issue 3, p36003-36003. 1p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Speckle+interference%22">Speckle interference</searchLink><br /><searchLink fieldCode="DE" term="%22Speckle+interferometry%22">Speckle interferometry</searchLink><br /><searchLink fieldCode="DE" term="%22Flow+sensors%22">Flow sensors</searchLink><br /><searchLink fieldCode="DE" term="%22Flow+measurement%22">Flow measurement</searchLink><br /><searchLink fieldCode="DE" term="%22Microfluidic+devices%22">Microfluidic devices</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Microfluidic flow phantom studies are commonly used for characterizing the performance of laser speckle contrast imaging (LSCI) instruments. The selection of the flow control system is critical for the reliable generation of flow during testing. The majority of recent LSCI studies using microfluidics used syringe pumps for flow control. We quantified the uncertainty in flow generation for a syringe pump and a pressure-regulated flow system. We then assessed the performance of both LSCI and multi-exposure speckle imaging (MESI) using the pressure-regulated flow system across a range of flow speeds. The syringe pump and pressure-regulated flow systems were evaluated during stepped flow profile experiments in a microfluidic device using an inline flow sensor. The uncertainty associated with each flow system was calculated and used to determine the reliability for instrument testing. The pressure-regulated flow system was then used to characterize the relative performance of LSCI and MESI during stepped flow profile experiments while using the inline flow sensor as reference. The pressure-regulated flow system produced much more stable and reproducible flow outputs compared to the syringe pump. The expanded uncertainty for the syringe pump was 8 to 20 × higher than that of the pressure-regulated flow system across the tested flow speeds. Using the pressure-regulated flow system, MESI outperformed single-exposure LSCI at all flow speeds and closely mirrored the flow sensor measurements, with average errors of 4.6 % ± 2.6 % and 15.7 % ± 4.6 % , respectively. Pressure-regulated flow systems should be used instead of syringe pumps when assessing the performance of flow measurement techniques with microfluidic studies. MESI offers more accurate relative flow measurements than traditional LSCI across a wide range of flow speeds. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Biomedical Optics is the property of SPIE - International Society of Optical Engineering and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=162800882
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1117/1.JBO.28.3.036003
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 1
        StartPage: 36003
    Subjects:
      – SubjectFull: Speckle interference
        Type: general
      – SubjectFull: Speckle interferometry
        Type: general
      – SubjectFull: Flow sensors
        Type: general
      – SubjectFull: Flow measurement
        Type: general
      – SubjectFull: Microfluidic devices
        Type: general
    Titles:
      – TitleFull: Using pressure-driven flow systems to evaluate laser speckle contrast imaging.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Sullender, Colin T.
      – PersonEntity:
          Name:
            NameFull: Santorelli, Adam
      – PersonEntity:
          Name:
            NameFull: Richards, Lisa M.
      – PersonEntity:
          Name:
            NameFull: Mannava, Pawan K.
      – PersonEntity:
          Name:
            NameFull: Smith, Christopher
      – PersonEntity:
          Name:
            NameFull: Dunn, Andrew K.
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 03
              Text: Mar2023
              Type: published
              Y: 2023
          Identifiers:
            – Type: issn-print
              Value: 10833668
          Numbering:
            – Type: volume
              Value: 28
            – Type: issue
              Value: 3
          Titles:
            – TitleFull: Journal of Biomedical Optics
              Type: main
ResultId 1