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3D printed polylactide scaffolding for laccase immobilization to improve enzyme stability and estrogen removal from wastewater.

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Title: 3D printed polylactide scaffolding for laccase immobilization to improve enzyme stability and estrogen removal from wastewater.
Authors: Rybarczyk, Agnieszka1 (AUTHOR), Smułek, Wojciech1 (AUTHOR), Grzywaczyk, Adam1 (AUTHOR), Kaczorek, Ewa1 (AUTHOR), Jesionowski, Teofil1 (AUTHOR), Nghiem, Long D.2 (AUTHOR), Zdarta, Jakub1 (AUTHOR) jakub.zdarta@put.poznan.pl
Source: Bioresource Technology. Aug2023, Vol. 381, pN.PAG-N.PAG. 1p.
Subjects: Enzyme stability, Laccase, Polylactic acid, Chemical stability, Sewage, Estrogen, Enzyme kinetics
Abstract: [Display omitted] • Production of stable biocatalytic system using open-structure 3D printed scaffolds. • 24 h, pH 5 and 5 mg/mL enzyme solution were optimal immobilization conditions. • High thermal and chemical stability of system with immobilized laccase. • Removal of over 30% of E2 and 40% of EE2 from wastewater using produced systems. • Wastewater matrix constituents affect enzymatic treatment of micropollutants. This study reports a biocatalytic system of immobilized laccase and 3D printed open-structure biopolymer scaffoldings. The scaffoldings were computer-designed and 3D printed using polylactide (PLA) filament. The immobilization of laccase onto the 3D printed PLA scaffolds were optimized with regard to pH, enzyme concentration, and immobilization time. Laccase immobilization resulted in a small reduction in reactivity (in terms of Michaelis constant and maximum reaction rate) but led to significant improvement in chemical and thermal stability. After 20 days of storage, the immobilized and free laccase showed 80% and 35% retention of the initial enzymatic activity, respectively. The immobilized laccase on 3D printed PLA scaffolds achieved 10% improvement in the removal of estrogens from real wastewater as compared to free laccase and showed the significant reusability potential. Results here are promising but also highlight the need for further study to improve enzymatic activity and reusability. [ABSTRACT FROM AUTHOR]
Copyright of Bioresource Technology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 163847736
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Label: Title
  Group: Ti
  Data: 3D printed polylactide scaffolding for laccase immobilization to improve enzyme stability and estrogen removal from wastewater.
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  Label: Authors
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  Data: <searchLink fieldCode="AR" term="%22Rybarczyk%2C+Agnieszka%22">Rybarczyk, Agnieszka</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Smułek%2C+Wojciech%22">Smułek, Wojciech</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Grzywaczyk%2C+Adam%22">Grzywaczyk, Adam</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kaczorek%2C+Ewa%22">Kaczorek, Ewa</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jesionowski%2C+Teofil%22">Jesionowski, Teofil</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Nghiem%2C+Long+D%2E%22">Nghiem, Long D.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zdarta%2C+Jakub%22">Zdarta, Jakub</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> jakub.zdarta@put.poznan.pl</i>
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  Data: <searchLink fieldCode="JN" term="%22Bioresource+Technology%22">Bioresource Technology</searchLink>. Aug2023, Vol. 381, pN.PAG-N.PAG. 1p.
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  Data: <searchLink fieldCode="DE" term="%22Enzyme+stability%22">Enzyme stability</searchLink><br /><searchLink fieldCode="DE" term="%22Laccase%22">Laccase</searchLink><br /><searchLink fieldCode="DE" term="%22Polylactic+acid%22">Polylactic acid</searchLink><br /><searchLink fieldCode="DE" term="%22Chemical+stability%22">Chemical stability</searchLink><br /><searchLink fieldCode="DE" term="%22Sewage%22">Sewage</searchLink><br /><searchLink fieldCode="DE" term="%22Estrogen%22">Estrogen</searchLink><br /><searchLink fieldCode="DE" term="%22Enzyme+kinetics%22">Enzyme kinetics</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: [Display omitted] • Production of stable biocatalytic system using open-structure 3D printed scaffolds. • 24 h, pH 5 and 5 mg/mL enzyme solution were optimal immobilization conditions. • High thermal and chemical stability of system with immobilized laccase. • Removal of over 30% of E2 and 40% of EE2 from wastewater using produced systems. • Wastewater matrix constituents affect enzymatic treatment of micropollutants. This study reports a biocatalytic system of immobilized laccase and 3D printed open-structure biopolymer scaffoldings. The scaffoldings were computer-designed and 3D printed using polylactide (PLA) filament. The immobilization of laccase onto the 3D printed PLA scaffolds were optimized with regard to pH, enzyme concentration, and immobilization time. Laccase immobilization resulted in a small reduction in reactivity (in terms of Michaelis constant and maximum reaction rate) but led to significant improvement in chemical and thermal stability. After 20 days of storage, the immobilized and free laccase showed 80% and 35% retention of the initial enzymatic activity, respectively. The immobilized laccase on 3D printed PLA scaffolds achieved 10% improvement in the removal of estrogens from real wastewater as compared to free laccase and showed the significant reusability potential. Results here are promising but also highlight the need for further study to improve enzymatic activity and reusability. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Bioresource Technology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.biortech.2023.129144
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Enzyme stability
        Type: general
      – SubjectFull: Laccase
        Type: general
      – SubjectFull: Polylactic acid
        Type: general
      – SubjectFull: Chemical stability
        Type: general
      – SubjectFull: Sewage
        Type: general
      – SubjectFull: Estrogen
        Type: general
      – SubjectFull: Enzyme kinetics
        Type: general
    Titles:
      – TitleFull: 3D printed polylactide scaffolding for laccase immobilization to improve enzyme stability and estrogen removal from wastewater.
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          Name:
            NameFull: Rybarczyk, Agnieszka
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            NameFull: Smułek, Wojciech
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            NameFull: Grzywaczyk, Adam
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            NameFull: Kaczorek, Ewa
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            NameFull: Jesionowski, Teofil
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            NameFull: Nghiem, Long D.
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            NameFull: Zdarta, Jakub
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            – D: 01
              M: 08
              Text: Aug2023
              Type: published
              Y: 2023
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            – Type: issn-print
              Value: 09608524
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              Value: 381
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            – TitleFull: Bioresource Technology
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