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Novel fluorescent nanoprobe based on hyaluronic acid and polyethyleneimine functionalized graphene oxide for detecting hyaluronidase as tumor marker.

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Title: Novel fluorescent nanoprobe based on hyaluronic acid and polyethyleneimine functionalized graphene oxide for detecting hyaluronidase as tumor marker.
Authors: Zhang, Ping1 (AUTHOR), Zhang, Chaoqun1 (AUTHOR), Song, Jie1 (AUTHOR), Wang, Shuxin1 (AUTHOR), Li, Qian1,2 (AUTHOR) liqian123@qdu.edu.cn, Su, Feng1,3 (AUTHOR) sufeng@qust.edu.cn, Li, Suming4 (AUTHOR) suming.li@umontpellier.fr
Source: Polymers for Advanced Technologies. Jul2023, Vol. 34 Issue 7, p2340-2354. 15p.
Subjects: Polyethyleneimine, Fluorescein, Hyaluronic acid, Graphene oxide, Tumor markers, Fourier transform infrared spectroscopy, Hyaluronidases
Abstract: A novel nanoprobe was developed for the detection of hyaluronidase as cancer marker, using fluorescein 5‐isothiocyanate (FITC) as fluorescence indicator, hyaluronic acid (HA), and polyethyleneimine (PEI) functionalized graphene oxide (GO) as quencher. PEI was attached to GO through amide bonding, using 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide (EDC) and N‐hydroxysuccinimide (NHS) as coupling agents. Aminated hyaluronic acid (HA) was then linked to GO‐PEI (GOP) via the same route. Nuclear magnetic resonance, ultraviolet–visible–near infrared spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscope, and thermogravimetric analysis analyses confirmed the successful synthesis of GOP‐HA. Finally, FITC was attached to GOP‐HA through reaction between the isothiocyanate group of FITC and the amine group of aminated HA. The fluorescence of FITC is quenched in the resulted GOP‐HA‐FITC nanoprobe. Addition of hyaluronidase can cleave HA chains into small fragments, releasing FITC to emit fluorescence. Importantly, the nanoprobe is highly sensitive and selective, and can enter cells by specifically binding to the CD44 receptor on the surface of Hela cells. Therefore, GOP‐HA‐FITC nanoprobe could be used in targeted tumor cell imaging for the early diagnosis of cancers. [ABSTRACT FROM AUTHOR]
Copyright of Polymers for Advanced Technologies is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Novel fluorescent nanoprobe based on hyaluronic acid and polyethyleneimine functionalized graphene oxide for detecting hyaluronidase as tumor marker.
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  Data: <searchLink fieldCode="AR" term="%22Zhang%2C+Ping%22">Zhang, Ping</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Chaoqun%22">Zhang, Chaoqun</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Song%2C+Jie%22">Song, Jie</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Shuxin%22">Wang, Shuxin</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Li%2C+Qian%22">Li, Qian</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> liqian123@qdu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Su%2C+Feng%22">Su, Feng</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)<i> sufeng@qust.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Li%2C+Suming%22">Li, Suming</searchLink><relatesTo>4</relatesTo> (AUTHOR)<i> suming.li@umontpellier.fr</i>
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  Data: <searchLink fieldCode="JN" term="%22Polymers+for+Advanced+Technologies%22">Polymers for Advanced Technologies</searchLink>. Jul2023, Vol. 34 Issue 7, p2340-2354. 15p.
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  Data: <searchLink fieldCode="DE" term="%22Polyethyleneimine%22">Polyethyleneimine</searchLink><br /><searchLink fieldCode="DE" term="%22Fluorescein%22">Fluorescein</searchLink><br /><searchLink fieldCode="DE" term="%22Hyaluronic+acid%22">Hyaluronic acid</searchLink><br /><searchLink fieldCode="DE" term="%22Graphene+oxide%22">Graphene oxide</searchLink><br /><searchLink fieldCode="DE" term="%22Tumor+markers%22">Tumor markers</searchLink><br /><searchLink fieldCode="DE" term="%22Fourier+transform+infrared+spectroscopy%22">Fourier transform infrared spectroscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Hyaluronidases%22">Hyaluronidases</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: A novel nanoprobe was developed for the detection of hyaluronidase as cancer marker, using fluorescein 5‐isothiocyanate (FITC) as fluorescence indicator, hyaluronic acid (HA), and polyethyleneimine (PEI) functionalized graphene oxide (GO) as quencher. PEI was attached to GO through amide bonding, using 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide (EDC) and N‐hydroxysuccinimide (NHS) as coupling agents. Aminated hyaluronic acid (HA) was then linked to GO‐PEI (GOP) via the same route. Nuclear magnetic resonance, ultraviolet–visible–near infrared spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscope, and thermogravimetric analysis analyses confirmed the successful synthesis of GOP‐HA. Finally, FITC was attached to GOP‐HA through reaction between the isothiocyanate group of FITC and the amine group of aminated HA. The fluorescence of FITC is quenched in the resulted GOP‐HA‐FITC nanoprobe. Addition of hyaluronidase can cleave HA chains into small fragments, releasing FITC to emit fluorescence. Importantly, the nanoprobe is highly sensitive and selective, and can enter cells by specifically binding to the CD44 receptor on the surface of Hela cells. Therefore, GOP‐HA‐FITC nanoprobe could be used in targeted tumor cell imaging for the early diagnosis of cancers. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Polymers for Advanced Technologies is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1002/pat.6055
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        Text: English
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        PageCount: 15
        StartPage: 2340
    Subjects:
      – SubjectFull: Polyethyleneimine
        Type: general
      – SubjectFull: Fluorescein
        Type: general
      – SubjectFull: Hyaluronic acid
        Type: general
      – SubjectFull: Graphene oxide
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      – SubjectFull: Tumor markers
        Type: general
      – SubjectFull: Fourier transform infrared spectroscopy
        Type: general
      – SubjectFull: Hyaluronidases
        Type: general
    Titles:
      – TitleFull: Novel fluorescent nanoprobe based on hyaluronic acid and polyethyleneimine functionalized graphene oxide for detecting hyaluronidase as tumor marker.
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            NameFull: Zhang, Ping
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            NameFull: Zhang, Chaoqun
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            NameFull: Song, Jie
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            NameFull: Wang, Shuxin
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            NameFull: Li, Qian
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            – D: 01
              M: 07
              Text: Jul2023
              Type: published
              Y: 2023
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              Value: 34
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