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Characterization of an endo‐beta‐1,4 glucanase gene from paper‐degrading and denim bio‐stoning cellulase producing Aspergillus isolates.

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Title: Characterization of an endo‐beta‐1,4 glucanase gene from paper‐degrading and denim bio‐stoning cellulase producing Aspergillus isolates.
Authors: Ahmed, Jahed1 (AUTHOR) jahed.geb@gmail.com, Taslim, Asma‐Ul‐1 (AUTHOR), Raihan, Topu1 (AUTHOR), Sohag, Md. Mehadi Hasan1 (AUTHOR), Hasan, Mahmudul1 (AUTHOR), Suhani, Sabrina1 (AUTHOR), Qadri, Firdausi2 (AUTHOR), Azad, Abul Kalam1 (AUTHOR) dakazad-btc@sust.edu
Source: Biotechnology & Applied Biochemistry. Jun2023, Vol. 70 Issue 3, p1057-1071. 15p.
Subjects: Cellulase, Aspergillus, Carboxymethylcellulose, Aspergillus flavus, Aspergillus fumigatus, Ribosomal DNA, Ethanol, Aflatoxins
Abstract: Cellulases are used in textile, pulp and paper, brewery and wine, sugars, and ethanol industries. Four fungal isolates obtained from organic municipal solid wastes (OMSW) were selected based on their cellulolytic activity on carboxymethyl cellulose (CMC) agar medium. Based on the internal transcribed spacer (ITS) sequence of the ribosomal DNA, the four cellulolytic isolates were identified as Aspergillus fumigatus AKAL1, Aspergillus oryzae AKAL4, Aspergillus flavus AKAL8, and Aspergillus flavus AKAL9. After 9 days of fermentation at 30°C and pH 6.5 under 110 rpm agitation, these isolates produced the maximum amount of cellulase. The cellulase showed optimum activity at temperature 35–40°C and pH 6.0–7.0 and was stable for 1 h at 25–45°C and pH 5.0–7.0. The Mg2+ and Zn2+ significantly increased but Hg2+, K+, and Ca2+ severely repressed the cellulase activity. Degradation of filter papers and bio‐stoning of denim was successfully done with the crude cellulase. An endo‐β‐1,4‐glucanase was isolated and characterized from Aspergillus isolates. Genome‐wide analysis revealed that the genomes of A. oryzae, A. fumigatus, and A. flavus, the pertinent species of the fungal isolates, had 23, 25, and 22 cellulase genes, respectively. Phylogenetic analysis revealed that the cellulases in these fungal species were divided into three major groups, and the isolated endo‐β‐1,4‐glucanase clustered to Group II. Ten different motifs are present in cellulases of the three species. Results herein provide a valuable resource for understanding cellulase genes in Aspergillus species and potential application of cellulase in textile and fermentable sugars production industries. [ABSTRACT FROM AUTHOR]
Copyright of Biotechnology & Applied Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Characterization of an endo‐beta‐1,4 glucanase gene from paper‐degrading and denim bio‐stoning cellulase producing Aspergillus isolates.
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  Data: <searchLink fieldCode="AR" term="%22Ahmed%2C+Jahed%22">Ahmed, Jahed</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> jahed.geb@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Taslim%2C+Asma‐Ul‐%22">Taslim, Asma‐Ul‐</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Raihan%2C+Topu%22">Raihan, Topu</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sohag%2C+Md%2E+Mehadi+Hasan%22">Sohag, Md. Mehadi Hasan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hasan%2C+Mahmudul%22">Hasan, Mahmudul</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Suhani%2C+Sabrina%22">Suhani, Sabrina</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Qadri%2C+Firdausi%22">Qadri, Firdausi</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Azad%2C+Abul+Kalam%22">Azad, Abul Kalam</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> dakazad-btc@sust.edu</i>
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  Data: <searchLink fieldCode="JN" term="%22Biotechnology+%26+Applied+Biochemistry%22">Biotechnology & Applied Biochemistry</searchLink>. Jun2023, Vol. 70 Issue 3, p1057-1071. 15p.
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  Data: <searchLink fieldCode="DE" term="%22Cellulase%22">Cellulase</searchLink><br /><searchLink fieldCode="DE" term="%22Aspergillus%22">Aspergillus</searchLink><br /><searchLink fieldCode="DE" term="%22Carboxymethylcellulose%22">Carboxymethylcellulose</searchLink><br /><searchLink fieldCode="DE" term="%22Aspergillus+flavus%22">Aspergillus flavus</searchLink><br /><searchLink fieldCode="DE" term="%22Aspergillus+fumigatus%22">Aspergillus fumigatus</searchLink><br /><searchLink fieldCode="DE" term="%22Ribosomal+DNA%22">Ribosomal DNA</searchLink><br /><searchLink fieldCode="DE" term="%22Ethanol%22">Ethanol</searchLink><br /><searchLink fieldCode="DE" term="%22Aflatoxins%22">Aflatoxins</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Cellulases are used in textile, pulp and paper, brewery and wine, sugars, and ethanol industries. Four fungal isolates obtained from organic municipal solid wastes (OMSW) were selected based on their cellulolytic activity on carboxymethyl cellulose (CMC) agar medium. Based on the internal transcribed spacer (ITS) sequence of the ribosomal DNA, the four cellulolytic isolates were identified as Aspergillus fumigatus AKAL1, Aspergillus oryzae AKAL4, Aspergillus flavus AKAL8, and Aspergillus flavus AKAL9. After 9 days of fermentation at 30°C and pH 6.5 under 110 rpm agitation, these isolates produced the maximum amount of cellulase. The cellulase showed optimum activity at temperature 35–40°C and pH 6.0–7.0 and was stable for 1 h at 25–45°C and pH 5.0–7.0. The Mg2+ and Zn2+ significantly increased but Hg2+, K+, and Ca2+ severely repressed the cellulase activity. Degradation of filter papers and bio‐stoning of denim was successfully done with the crude cellulase. An endo‐β‐1,4‐glucanase was isolated and characterized from Aspergillus isolates. Genome‐wide analysis revealed that the genomes of A. oryzae, A. fumigatus, and A. flavus, the pertinent species of the fungal isolates, had 23, 25, and 22 cellulase genes, respectively. Phylogenetic analysis revealed that the cellulases in these fungal species were divided into three major groups, and the isolated endo‐β‐1,4‐glucanase clustered to Group II. Ten different motifs are present in cellulases of the three species. Results herein provide a valuable resource for understanding cellulase genes in Aspergillus species and potential application of cellulase in textile and fermentable sugars production industries. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Biotechnology & Applied Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1002/bab.2420
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 15
        StartPage: 1057
    Subjects:
      – SubjectFull: Cellulase
        Type: general
      – SubjectFull: Aspergillus
        Type: general
      – SubjectFull: Carboxymethylcellulose
        Type: general
      – SubjectFull: Aspergillus flavus
        Type: general
      – SubjectFull: Aspergillus fumigatus
        Type: general
      – SubjectFull: Ribosomal DNA
        Type: general
      – SubjectFull: Ethanol
        Type: general
      – SubjectFull: Aflatoxins
        Type: general
    Titles:
      – TitleFull: Characterization of an endo‐beta‐1,4 glucanase gene from paper‐degrading and denim bio‐stoning cellulase producing Aspergillus isolates.
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            NameFull: Ahmed, Jahed
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            NameFull: Taslim, Asma‐Ul‐
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            NameFull: Raihan, Topu
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            NameFull: Sohag, Md. Mehadi Hasan
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            NameFull: Hasan, Mahmudul
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            NameFull: Suhani, Sabrina
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            NameFull: Azad, Abul Kalam
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            – D: 01
              M: 06
              Text: Jun2023
              Type: published
              Y: 2023
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              Value: 70
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            – TitleFull: Biotechnology & Applied Biochemistry
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