Targeting the aluminum tolerance geneAlt3region in rye, using rice/rye micro-colinearity.
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| Title: | Targeting the aluminum tolerance geneAlt3region in rye, using rice/rye micro-colinearity. |
|---|---|
| Authors: | Miftahudin1,2, Chikmawati, T.1,2, Ross, K.1,3, Scoles, G.2,4, Gustafson, J.1,3 pgus@missouri.edu |
| Source: | Theoretical & Applied Genetics. Mar2005, Vol. 110 Issue 5, p906-913. 8p. |
| Subjects: | Rice genetics, Rye, Genetics, Genetic engineering, Gene mapping, Plant gene mapping |
| Abstract: | Characterization and manipulation of aluminum (Al) tolerance genes offers a solution to Al toxicity problems in crop cultivation on acid soil, which composes approximately 40% of all arable land. By exploiting the rice (Oryza sativaL.)/rye (Secale cerealeL.) syntenic relationship, the potential for map-based cloning of genes controlling Al tolerance in rye (the most Al-tolerant cereal) was explored. An attempt to clone an Al tolerance gene (Alt3) from rye was initiated by using DNA markers flanking the ryeAlt3gene, from many cereals. Two rice-derived, PCR-based markers flanking theAlt3gene, B1 and B4, were used to screen 1,123 plants of a rye F2 population segregating forAlt3. Fifteen recombinant plants were identified. Four additional RFLP markers developed from rice genes/putative genes, spanning 10 kb of a 160-kb rice BAC, were mapped to theAlt3region. Two rice markers flanked theAlt3locus at a distance of 0.05 cM, while two others co-segregated with it. The rice/rye micro-colinearity worked very well to delineate and map theAlt3gene region in rye. A rye fragment suspected to be part of theAlt3candidate gene was identified, but at this level, the rye/rice microsynteny relationship broke down. Because of sequence differences between rice and rye and the complexity of the rye sequence, we have been unable to clone a full-length candidate gene in rye. Further attempts to clone a full-length ryeAlt3candidate gene will necessitate the creation of a rye large-insert library. [ABSTRACT FROM AUTHOR] |
| Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 16573627 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Targeting the aluminum tolerance geneAlt3region in rye, using rice/rye micro-colinearity. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Miftahudin%22">Miftahudin</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Chikmawati%2C+T%2E%22">Chikmawati, T.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Ross%2C+K%2E%22">Ross, K.</searchLink><relatesTo>1,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Scoles%2C+G%2E%22">Scoles, G.</searchLink><relatesTo>2,4</relatesTo><br /><searchLink fieldCode="AR" term="%22Gustafson%2C+J%2E%22">Gustafson, J.</searchLink><relatesTo>1,3</relatesTo><i> pgus@missouri.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Theoretical+%26+Applied+Genetics%22">Theoretical & Applied Genetics</searchLink>. Mar2005, Vol. 110 Issue 5, p906-913. 8p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Rice+genetics%22">Rice genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Rye%22">Rye</searchLink><br /><searchLink fieldCode="DE" term="%22Genetics%22">Genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+engineering%22">Genetic engineering</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+mapping%22">Gene mapping</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+gene+mapping%22">Plant gene mapping</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Characterization and manipulation of aluminum (Al) tolerance genes offers a solution to Al toxicity problems in crop cultivation on acid soil, which composes approximately 40% of all arable land. By exploiting the rice (Oryza sativaL.)/rye (Secale cerealeL.) syntenic relationship, the potential for map-based cloning of genes controlling Al tolerance in rye (the most Al-tolerant cereal) was explored. An attempt to clone an Al tolerance gene (Alt3) from rye was initiated by using DNA markers flanking the ryeAlt3gene, from many cereals. Two rice-derived, PCR-based markers flanking theAlt3gene, B1 and B4, were used to screen 1,123 plants of a rye F2 population segregating forAlt3. Fifteen recombinant plants were identified. Four additional RFLP markers developed from rice genes/putative genes, spanning 10 kb of a 160-kb rice BAC, were mapped to theAlt3region. Two rice markers flanked theAlt3locus at a distance of 0.05 cM, while two others co-segregated with it. The rice/rye micro-colinearity worked very well to delineate and map theAlt3gene region in rye. A rye fragment suspected to be part of theAlt3candidate gene was identified, but at this level, the rye/rice microsynteny relationship broke down. Because of sequence differences between rice and rye and the complexity of the rye sequence, we have been unable to clone a full-length candidate gene in rye. Further attempts to clone a full-length ryeAlt3candidate gene will necessitate the creation of a rye large-insert library. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00122-004-1909-0 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 8 StartPage: 906 Subjects: – SubjectFull: Rice genetics Type: general – SubjectFull: Rye Type: general – SubjectFull: Genetics Type: general – SubjectFull: Genetic engineering Type: general – SubjectFull: Gene mapping Type: general – SubjectFull: Plant gene mapping Type: general Titles: – TitleFull: Targeting the aluminum tolerance geneAlt3region in rye, using rice/rye micro-colinearity. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Miftahudin – PersonEntity: Name: NameFull: Chikmawati, T. – PersonEntity: Name: NameFull: Ross, K. – PersonEntity: Name: NameFull: Scoles, G. – PersonEntity: Name: NameFull: Gustafson, J. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: Mar2005 Type: published Y: 2005 Identifiers: – Type: issn-print Value: 00405752 Numbering: – Type: volume Value: 110 – Type: issue Value: 5 Titles: – TitleFull: Theoretical & Applied Genetics Type: main |
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