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Continuous multi-membrane chromatography of large viral particles.

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Title: Continuous multi-membrane chromatography of large viral particles.
Authors: Matos, Tiago1 (AUTHOR) Tiago.Matos@merck.com, Hoying, David1 (AUTHOR), Kristopeit, Adam1 (AUTHOR), Wenger, Marc1 (AUTHOR), Joyce, Joseph1 (AUTHOR)
Source: Journal of Chromatography A. Aug2023, Vol. 1705, pN.PAG-N.PAG. 1p.
Subjects: Chromatographic analysis, Viral vaccines, Vaccine manufacturing, Capital costs
Abstract: • Membrane chromatography for large viral particles purification. • Multi-membrane chromatography through periodic counter current method. • Continuous manufacturing for viral vaccines. Continuous multi-column chromatography (CMCC) has been successfully implemented to address biopharmaceutical biomolecule instability, to improve process efficiency, and to reduce facility footprint and capital cost. This paper explores the implementation of a continuous multi-membrane chromatography (CMMC) process, using four membrane units, for a large viral particle in just few weeks. CMMC improves the efficiency of the chromatography step by enabling higher loads with smaller membranes for multiple cycles of column use and enables steady-state continuous bioprocessing. The separation performance of CMMC was compared to a conventional batch chromatographic capture step used at full manufacturing scale. The product step yield was 80% using CMMC versus 65% in batch mode while increasing slightly the relative purity. Furthermore, the total amount of membrane area required for the CMMC approach was approximately 10% of the area needed for batch operation, while realizing similar processing times. Since CMMC uses smaller membrane sizes, it can take advantage of the high flow rates achievable for membrane chromatography that are not typically possible at larger membrane scales due to skid flow rate limitations. As such, CMMC offers the potential for more efficient and cost-effective purification trains. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 169789522
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Data: Continuous multi-membrane chromatography of large viral particles.
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  Data: <searchLink fieldCode="DE" term="%22Chromatographic+analysis%22">Chromatographic analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Viral+vaccines%22">Viral vaccines</searchLink><br /><searchLink fieldCode="DE" term="%22Vaccine+manufacturing%22">Vaccine manufacturing</searchLink><br /><searchLink fieldCode="DE" term="%22Capital+costs%22">Capital costs</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: • Membrane chromatography for large viral particles purification. • Multi-membrane chromatography through periodic counter current method. • Continuous manufacturing for viral vaccines. Continuous multi-column chromatography (CMCC) has been successfully implemented to address biopharmaceutical biomolecule instability, to improve process efficiency, and to reduce facility footprint and capital cost. This paper explores the implementation of a continuous multi-membrane chromatography (CMMC) process, using four membrane units, for a large viral particle in just few weeks. CMMC improves the efficiency of the chromatography step by enabling higher loads with smaller membranes for multiple cycles of column use and enables steady-state continuous bioprocessing. The separation performance of CMMC was compared to a conventional batch chromatographic capture step used at full manufacturing scale. The product step yield was 80% using CMMC versus 65% in batch mode while increasing slightly the relative purity. Furthermore, the total amount of membrane area required for the CMMC approach was approximately 10% of the area needed for batch operation, while realizing similar processing times. Since CMMC uses smaller membrane sizes, it can take advantage of the high flow rates achievable for membrane chromatography that are not typically possible at larger membrane scales due to skid flow rate limitations. As such, CMMC offers the potential for more efficient and cost-effective purification trains. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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    Identifiers:
      – Type: doi
        Value: 10.1016/j.chroma.2023.464194
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      – Code: eng
        Text: English
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        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Chromatographic analysis
        Type: general
      – SubjectFull: Viral vaccines
        Type: general
      – SubjectFull: Vaccine manufacturing
        Type: general
      – SubjectFull: Capital costs
        Type: general
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      – TitleFull: Continuous multi-membrane chromatography of large viral particles.
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            NameFull: Kristopeit, Adam
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            NameFull: Wenger, Marc
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            NameFull: Joyce, Joseph
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            – D: 30
              M: 08
              Text: Aug2023
              Type: published
              Y: 2023
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              Value: 1705
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            – TitleFull: Journal of Chromatography A
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