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Development of chloroplast DNA markers for environmental monitoring of genetically modified Brassica napus and Brassica hybrids.

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Title: Development of chloroplast DNA markers for environmental monitoring of genetically modified Brassica napus and Brassica hybrids.
Authors: Lee, Bumkyu1 (AUTHOR) leebk@jj.ac.kr, Sohn, Soo-In2 (AUTHOR) sisohn@korea.kr
Source: Plant Biotechnology Reports. Aug2023, Vol. 17 Issue 4, p489-497. 9p.
Subjects: Rapeseed, Environmental monitoring, Chloroplasts, Genetic markers, Brassica juncea, Chloroplast DNA, Polymerase chain reaction
Abstract: The increasing cultivation and use of genetically modified (GM) crops has increased the importance of environmental monitoring for safety management. Brassica napus is the fourth most commonly cultivated GM crop globally, and it can hybridize with Brassica rapa or Brassica juncea by serving as a pollen donor or recipient for these species. When a hybrid of B. rapa or B. juncea with GM B. napus is detected in an outdoor environment, the safety management goals could determine whether B. napus is a pollen donor or recipient. In this study, two PCR (polymerase chain reaction) markers were developed to identify the paternal and maternal involvement of B. napus in hybrids with B. rapa or B. juncea based on the chloroplast genome data of B. napus. Further, Marker primer 07 differentiated B. napus from the other Brassica species, and Marker primer 20 differentiated B. napus from B. rapa and B. juncea. Applying the chloroplast and nuclear markers of the C and B genomes to the hybrids B. napus × B. rapa, B. rapa × B. napus, and B. juncea × B. napus using the PCR method showed that B. napus, B. rapa, B. juncea, and all hybrids could be distinguished. Thus, the effectiveness of these markers in identifying hybrids of GM B. napus in the natural environment has been demonstrated for environmental monitoring. [ABSTRACT FROM AUTHOR]
Copyright of Plant Biotechnology Reports is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Development of chloroplast DNA markers for environmental monitoring of genetically modified Brassica napus and Brassica hybrids.
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  Data: <searchLink fieldCode="AR" term="%22Lee%2C+Bumkyu%22">Lee, Bumkyu</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> leebk@jj.ac.kr</i><br /><searchLink fieldCode="AR" term="%22Sohn%2C+Soo-In%22">Sohn, Soo-In</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> sisohn@korea.kr</i>
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  Data: <searchLink fieldCode="JN" term="%22Plant+Biotechnology+Reports%22">Plant Biotechnology Reports</searchLink>. Aug2023, Vol. 17 Issue 4, p489-497. 9p.
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  Data: <searchLink fieldCode="DE" term="%22Rapeseed%22">Rapeseed</searchLink><br /><searchLink fieldCode="DE" term="%22Environmental+monitoring%22">Environmental monitoring</searchLink><br /><searchLink fieldCode="DE" term="%22Chloroplasts%22">Chloroplasts</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+markers%22">Genetic markers</searchLink><br /><searchLink fieldCode="DE" term="%22Brassica+juncea%22">Brassica juncea</searchLink><br /><searchLink fieldCode="DE" term="%22Chloroplast+DNA%22">Chloroplast DNA</searchLink><br /><searchLink fieldCode="DE" term="%22Polymerase+chain+reaction%22">Polymerase chain reaction</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The increasing cultivation and use of genetically modified (GM) crops has increased the importance of environmental monitoring for safety management. Brassica napus is the fourth most commonly cultivated GM crop globally, and it can hybridize with Brassica rapa or Brassica juncea by serving as a pollen donor or recipient for these species. When a hybrid of B. rapa or B. juncea with GM B. napus is detected in an outdoor environment, the safety management goals could determine whether B. napus is a pollen donor or recipient. In this study, two PCR (polymerase chain reaction) markers were developed to identify the paternal and maternal involvement of B. napus in hybrids with B. rapa or B. juncea based on the chloroplast genome data of B. napus. Further, Marker primer 07 differentiated B. napus from the other Brassica species, and Marker primer 20 differentiated B. napus from B. rapa and B. juncea. Applying the chloroplast and nuclear markers of the C and B genomes to the hybrids B. napus × B. rapa, B. rapa × B. napus, and B. juncea × B. napus using the PCR method showed that B. napus, B. rapa, B. juncea, and all hybrids could be distinguished. Thus, the effectiveness of these markers in identifying hybrids of GM B. napus in the natural environment has been demonstrated for environmental monitoring. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Plant Biotechnology Reports is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1007/s11816-023-00848-3
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      – Code: eng
        Text: English
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      – SubjectFull: Rapeseed
        Type: general
      – SubjectFull: Environmental monitoring
        Type: general
      – SubjectFull: Chloroplasts
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      – SubjectFull: Genetic markers
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      – SubjectFull: Brassica juncea
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      – SubjectFull: Chloroplast DNA
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      – SubjectFull: Polymerase chain reaction
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      – TitleFull: Development of chloroplast DNA markers for environmental monitoring of genetically modified Brassica napus and Brassica hybrids.
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            NameFull: Lee, Bumkyu
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            NameFull: Sohn, Soo-In
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              M: 08
              Text: Aug2023
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              Y: 2023
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