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Comparative sedimentation equilibrium analysis of two IgG1 glycoforms: IgGCri and IgGWid.

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Title: Comparative sedimentation equilibrium analysis of two IgG1 glycoforms: IgGCri and IgGWid.
Authors: Abu Hammad, Khalil1 (AUTHOR), Dinu, Vlad1 (AUTHOR), MacCalman, Thomas E.1,2 (AUTHOR), Pattem, Jacob1,2 (AUTHOR), Goodall, Margaret3 (AUTHOR), Gillis, Richard B.1,2,4 (AUTHOR), Jefferis, Roy3 (AUTHOR), Harding, Stephen E.1 (AUTHOR) steve.harding@nottingham.ac.uk
Source: European Biophysics Journal. Jul2023, Vol. 52 Issue 4/5, p439-443. 5p.
Subjects: Sedimentation analysis, Molar mass, Molecular weights, Velocity measurements, Sedimentation & deposition, Glycans
Abstract: The solution properties of two different glycoforms of IgG1 (IgG1Cri and IgG1Wid) are compared using primarily sedimentation equilibrium analysis with two complementary analysis routines: SEDFIT-MSTAR and MULTISIG. IgGCri bears diantennary complex-type glycans on its Fc domain that are fully core fucosylated and partially sialylated, whilst on IgGWid, they are non-fucosylated, partially galactosylated and non-sialylated. IgGWid is also Fab glycosylated. Despite these differences, SEDFIT-MSTAR analysis shows similar weight average molar masses Mw of ~ (150 ± 5) kDa for IgGCri and ~ (154 ± 5) kDa for IgGWid and both glycoforms show evidence of the presence of a small fraction of dimer confirmed by MULTISIG analysis and also by sedimentation coefficient distributions from supportive sedimentation velocity measurements. The closeness of the sedimentation equilibrium behaviour and sedimentation coefficient distributions with a main peak sedimentation coefficient of ~ 6.4S for both glycoforms at different concentrations suggest that the different glycosylation profiles do not significantly impact on molar mass (molecular weight) nor conformation in solution. [ABSTRACT FROM AUTHOR]
Copyright of European Biophysics Journal is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Comparative sedimentation equilibrium analysis of two IgG1 glycoforms: IgGCri and IgGWid.
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  Data: <searchLink fieldCode="JN" term="%22European+Biophysics+Journal%22">European Biophysics Journal</searchLink>. Jul2023, Vol. 52 Issue 4/5, p439-443. 5p.
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  Data: <searchLink fieldCode="DE" term="%22Sedimentation+analysis%22">Sedimentation analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Molar+mass%22">Molar mass</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+weights%22">Molecular weights</searchLink><br /><searchLink fieldCode="DE" term="%22Velocity+measurements%22">Velocity measurements</searchLink><br /><searchLink fieldCode="DE" term="%22Sedimentation+%26+deposition%22">Sedimentation & deposition</searchLink><br /><searchLink fieldCode="DE" term="%22Glycans%22">Glycans</searchLink>
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  Label: Abstract
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  Data: The solution properties of two different glycoforms of IgG1 (IgG1Cri and IgG1Wid) are compared using primarily sedimentation equilibrium analysis with two complementary analysis routines: SEDFIT-MSTAR and MULTISIG. IgGCri bears diantennary complex-type glycans on its Fc domain that are fully core fucosylated and partially sialylated, whilst on IgGWid, they are non-fucosylated, partially galactosylated and non-sialylated. IgGWid is also Fab glycosylated. Despite these differences, SEDFIT-MSTAR analysis shows similar weight average molar masses Mw of ~ (150 ± 5) kDa for IgGCri and ~ (154 ± 5) kDa for IgGWid and both glycoforms show evidence of the presence of a small fraction of dimer confirmed by MULTISIG analysis and also by sedimentation coefficient distributions from supportive sedimentation velocity measurements. The closeness of the sedimentation equilibrium behaviour and sedimentation coefficient distributions with a main peak sedimentation coefficient of ~ 6.4S for both glycoforms at different concentrations suggest that the different glycosylation profiles do not significantly impact on molar mass (molecular weight) nor conformation in solution. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of European Biophysics Journal is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1007/s00249-023-01656-x
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        Text: English
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      – SubjectFull: Molecular weights
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      – SubjectFull: Velocity measurements
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      – SubjectFull: Sedimentation & deposition
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      – SubjectFull: Glycans
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      – TitleFull: Comparative sedimentation equilibrium analysis of two IgG1 glycoforms: IgGCri and IgGWid.
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              Text: Jul2023
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