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Effect of enzymatic treatments and microfiltration on the physicochemical properties and antioxidant activities of two Tunisian prickly pear juices.

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Title: Effect of enzymatic treatments and microfiltration on the physicochemical properties and antioxidant activities of two Tunisian prickly pear juices.
Authors: Kallel, Fatma1 (AUTHOR) F111fatma@yahoo.fr, Chaibi, Zeinab1 (AUTHOR), Neifar, Mohamed1 (AUTHOR), Chaabouni, Semia Ellouz1,2 (AUTHOR)
Source: Process Biochemistry. Sep2023, Vol. 132, p140-151. 12p.
Subjects: Antioxidants, Opuntia, Microfiltration, Fruit juices, Pectic enzymes, Flavonoids
Abstract: Prickly pear has attracted domestic and international industries' attention owing to its nutritional and health benefit potentials. However, consumption of prickly pear juices is limited by many factors which are addressed essentially through processing methods. The purpose of this study was to compare the effect of clarification by pectinolytic enzymes of Penicillium occitanis coupled with microfiltration (MF) on the physicochemical properties and antioxidant activities of prickly pear juices. The juices were treated with various enzyme concentrations (0.5–5 U/mL), at different times (10–40 min) and temperatures (35–65 °C). The effect of these enzymatic treatments on the viscosity of juices was evaluated using a central composite design. The optimum conditions for the clarification of red fruit juice (RFJ) and green fruit juice (GFJ) were obtained based on surface and contour plots. The optimal treatment conditions were as follows: enzyme concentration 4.75 U/mL, time 37 min and temperature 55 °C for RFJ and enzyme concentration 4.65 U/mL, time 35 min and temperature 55 °C for GFJ. The experimental value of viscosity of the two prickly pear juices was reduced by 34.1% and 30%, respectively which was closely linked to the predicted value (31.79% and 28.93%, respectively). Higher permeate flux and better juice quality were achieved after the enzymatic treatment. The total phenolic and flavonoid contents were correlated with the antioxidant activities. During three months of storage at 4 °C, the clarified juices were microbiologically safe. Our findings showed that the combined pectinolytic enzymes-MF process can be considered as a promising technique that satisfactorily preserves the juice quality. [Display omitted] • Differences in the chemical composition of two prickly pear juices were observed. • The pectinase treatment conditions were optimized by RSM on the viscosity of juices. • The optimized treatment with pectinase was followed by microfiltration (MF). • Clear and stable prickly pear juices were obtained. [ABSTRACT FROM AUTHOR]
Copyright of Process Biochemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 170722065
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PubType: Academic Journal
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  Label: Title
  Group: Ti
  Data: Effect of enzymatic treatments and microfiltration on the physicochemical properties and antioxidant activities of two Tunisian prickly pear juices.
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  Label: Authors
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  Data: <searchLink fieldCode="AR" term="%22Kallel%2C+Fatma%22">Kallel, Fatma</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> F111fatma@yahoo.fr</i><br /><searchLink fieldCode="AR" term="%22Chaibi%2C+Zeinab%22">Chaibi, Zeinab</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Neifar%2C+Mohamed%22">Neifar, Mohamed</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chaabouni%2C+Semia+Ellouz%22">Chaabouni, Semia Ellouz</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22Process+Biochemistry%22">Process Biochemistry</searchLink>. Sep2023, Vol. 132, p140-151. 12p.
– Name: Subject
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  Data: <searchLink fieldCode="DE" term="%22Antioxidants%22">Antioxidants</searchLink><br /><searchLink fieldCode="DE" term="%22Opuntia%22">Opuntia</searchLink><br /><searchLink fieldCode="DE" term="%22Microfiltration%22">Microfiltration</searchLink><br /><searchLink fieldCode="DE" term="%22Fruit+juices%22">Fruit juices</searchLink><br /><searchLink fieldCode="DE" term="%22Pectic+enzymes%22">Pectic enzymes</searchLink><br /><searchLink fieldCode="DE" term="%22Flavonoids%22">Flavonoids</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Prickly pear has attracted domestic and international industries' attention owing to its nutritional and health benefit potentials. However, consumption of prickly pear juices is limited by many factors which are addressed essentially through processing methods. The purpose of this study was to compare the effect of clarification by pectinolytic enzymes of Penicillium occitanis coupled with microfiltration (MF) on the physicochemical properties and antioxidant activities of prickly pear juices. The juices were treated with various enzyme concentrations (0.5–5 U/mL), at different times (10–40 min) and temperatures (35–65 °C). The effect of these enzymatic treatments on the viscosity of juices was evaluated using a central composite design. The optimum conditions for the clarification of red fruit juice (RFJ) and green fruit juice (GFJ) were obtained based on surface and contour plots. The optimal treatment conditions were as follows: enzyme concentration 4.75 U/mL, time 37 min and temperature 55 °C for RFJ and enzyme concentration 4.65 U/mL, time 35 min and temperature 55 °C for GFJ. The experimental value of viscosity of the two prickly pear juices was reduced by 34.1% and 30%, respectively which was closely linked to the predicted value (31.79% and 28.93%, respectively). Higher permeate flux and better juice quality were achieved after the enzymatic treatment. The total phenolic and flavonoid contents were correlated with the antioxidant activities. During three months of storage at 4 °C, the clarified juices were microbiologically safe. Our findings showed that the combined pectinolytic enzymes-MF process can be considered as a promising technique that satisfactorily preserves the juice quality. [Display omitted] • Differences in the chemical composition of two prickly pear juices were observed. • The pectinase treatment conditions were optimized by RSM on the viscosity of juices. • The optimized treatment with pectinase was followed by microfiltration (MF). • Clear and stable prickly pear juices were obtained. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Process Biochemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.procbio.2023.07.016
    Languages:
      – Code: eng
        Text: English
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      Pagination:
        PageCount: 12
        StartPage: 140
    Subjects:
      – SubjectFull: Antioxidants
        Type: general
      – SubjectFull: Opuntia
        Type: general
      – SubjectFull: Microfiltration
        Type: general
      – SubjectFull: Fruit juices
        Type: general
      – SubjectFull: Pectic enzymes
        Type: general
      – SubjectFull: Flavonoids
        Type: general
    Titles:
      – TitleFull: Effect of enzymatic treatments and microfiltration on the physicochemical properties and antioxidant activities of two Tunisian prickly pear juices.
        Type: main
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      – PersonEntity:
          Name:
            NameFull: Kallel, Fatma
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            NameFull: Chaibi, Zeinab
      – PersonEntity:
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            NameFull: Neifar, Mohamed
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            NameFull: Chaabouni, Semia Ellouz
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          Dates:
            – D: 01
              M: 09
              Text: Sep2023
              Type: published
              Y: 2023
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            – Type: volume
              Value: 132
          Titles:
            – TitleFull: Process Biochemistry
              Type: main
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