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Enhanced invertase binding from baker's yeast via cryogels included boronic acids.

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Title: Enhanced invertase binding from baker's yeast via cryogels included boronic acids.
Authors: Baydemir Peşint, Gözde1 (AUTHOR) gpesint@atu.edu.tr, Eren Yüngeviş, Burcu1 (AUTHOR), Perçin Demirçelik, Işık2 (AUTHOR)
Source: World Journal of Microbiology & Biotechnology. Oct2023, Vol. 39 Issue 10, p1-13. 13p.
Subjects: Saccharomyces cerevisiae, Invertase, Scanning electron microscopy, Boronic acids, Infrared spectroscopy, Macroporous polymers
Abstract: Invertase, an industrially significant glycoenzyme, was purified from baker's yeast using poly (2-Hydroxyethyl methacrylate) [PHema-Pba] cryogels functionalized with boronic acid. At subzero temperatures, PHema-Pba cryogels were synthesized and characterized using swelling tests, scanning electron microscopy, and Fourier-transform infrared spectroscopy. The surface area of the PHema-Pba cryogels was 14 m2/g with a swelling ratio of 88.3% and macroporosity of 72%. The interconnected macropores of PHema-Pba cryogels were shown via scanning electron microscopy. Invertase binding capacity of PHema-Pba cryogel was evaluated by binding studies in different pH, temperature, and interaction time conditions and the maximum Invertase binding of PHema-Pba cryogel was found as 15.2 mg/g. and 23.7 fold Invertase purification was achieved from baker's yeast using PHema-Pba cryogels. The results show that PHema-Pba cryogels have high Invertase binding capacity and may be used as an alternative method for enzyme purification via boronate affinity systems. [ABSTRACT FROM AUTHOR]
Copyright of World Journal of Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Enhanced invertase binding from baker's yeast via cryogels included boronic acids.
– Name: Author
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  Data: <searchLink fieldCode="AR" term="%22Baydemir+Peşint%2C+Gözde%22">Baydemir Peşint, Gözde</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> gpesint@atu.edu.tr</i><br /><searchLink fieldCode="AR" term="%22Eren+Yüngeviş%2C+Burcu%22">Eren Yüngeviş, Burcu</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Perçin+Demirçelik%2C+Işık%22">Perçin Demirçelik, Işık</searchLink><relatesTo>2</relatesTo> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22World+Journal+of+Microbiology+%26+Biotechnology%22">World Journal of Microbiology & Biotechnology</searchLink>. Oct2023, Vol. 39 Issue 10, p1-13. 13p.
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  Data: <searchLink fieldCode="DE" term="%22Saccharomyces+cerevisiae%22">Saccharomyces cerevisiae</searchLink><br /><searchLink fieldCode="DE" term="%22Invertase%22">Invertase</searchLink><br /><searchLink fieldCode="DE" term="%22Scanning+electron+microscopy%22">Scanning electron microscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Boronic+acids%22">Boronic acids</searchLink><br /><searchLink fieldCode="DE" term="%22Infrared+spectroscopy%22">Infrared spectroscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Macroporous+polymers%22">Macroporous polymers</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Invertase, an industrially significant glycoenzyme, was purified from baker's yeast using poly (2-Hydroxyethyl methacrylate) [PHema-Pba] cryogels functionalized with boronic acid. At subzero temperatures, PHema-Pba cryogels were synthesized and characterized using swelling tests, scanning electron microscopy, and Fourier-transform infrared spectroscopy. The surface area of the PHema-Pba cryogels was 14 m2/g with a swelling ratio of 88.3% and macroporosity of 72%. The interconnected macropores of PHema-Pba cryogels were shown via scanning electron microscopy. Invertase binding capacity of PHema-Pba cryogel was evaluated by binding studies in different pH, temperature, and interaction time conditions and the maximum Invertase binding of PHema-Pba cryogel was found as 15.2 mg/g. and 23.7 fold Invertase purification was achieved from baker's yeast using PHema-Pba cryogels. The results show that PHema-Pba cryogels have high Invertase binding capacity and may be used as an alternative method for enzyme purification via boronate affinity systems. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of World Journal of Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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        Value: 10.1007/s11274-023-03697-y
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      – Code: eng
        Text: English
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      – SubjectFull: Saccharomyces cerevisiae
        Type: general
      – SubjectFull: Invertase
        Type: general
      – SubjectFull: Scanning electron microscopy
        Type: general
      – SubjectFull: Boronic acids
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      – SubjectFull: Infrared spectroscopy
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      – SubjectFull: Macroporous polymers
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      – TitleFull: Enhanced invertase binding from baker's yeast via cryogels included boronic acids.
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            NameFull: Eren Yüngeviş, Burcu
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            NameFull: Perçin Demirçelik, Işık
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            – D: 01
              M: 10
              Text: Oct2023
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              Y: 2023
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