Ultrasensitive quantitation of Paraquat based on a small molecule-induced dual-cycle amplification strategy.
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| Title: | Ultrasensitive quantitation of Paraquat based on a small molecule-induced dual-cycle amplification strategy. |
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| Authors: | Chen, Hao-Ran1 (AUTHOR), Zhang, Shun2,3 (AUTHOR), Chen, Tao1 (AUTHOR), Yang, Wei-Guo1 (AUTHOR), Su, Ming-Li1 (AUTHOR), Fu, Guan-Yan2 (AUTHOR), Yi, Wei-Jing3 (AUTHOR), Yuan, Ruo1 (AUTHOR), Xu, Shang-Cheng1,2 (AUTHOR) xushangchengmito@163.com, Liang, Wen-Bin1 (AUTHOR) wenbinliangasu@gmail.com |
| Source: | Biosensors & Bioelectronics. Nov2023, Vol. 240, pN.PAG-N.PAG. 1p. |
| Subjects: | Paraquat, Pesticide residues in food, Organophosphorus pesticides, Small molecules, Pesticide pollution, CRISPRs |
| Abstract: | Paraquat (PQ) is a typical biotoxic small molecule. Knowledge of how to directly introduce it into cyclic amplification rather than transform it into a secondary target is lacking in current analytical methods. Considering the urgent need for trace pesticide residue detection and the inherent defects of small molecule analysis, a CRISPR/Cas12a-driven small molecule-induced dual-cycle strategy was developed based on the immune competition method. The key to signal amplification is the mutual activation and acceleration between Cycle 1 triggered by the small molecule and Cycle 2 driven by CRISPR/Cas12a. Impressively, small molecules have been successfully incorporated into the dual-cycle strategy, which achieves a low detection limit (3.1 pg/mL) and a wide linear range (from 10 pg/mL to 50 μg/mL). Moreover, the designed biosensor was successfully employed to evaluate the PQ residual level in real samples and showed effective implementation for the bioanalysis of small molecule targets and pesticide residue-related food safety. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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