Toxic Interaction Between Solar Radiation and Cigarette Smoke on Primary Human Keratinocytes.
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| Title: | Toxic Interaction Between Solar Radiation and Cigarette Smoke on Primary Human Keratinocytes. |
|---|---|
| Authors: | Grenier, Alexe1,2 (AUTHOR), Morissette, Mathieu C.3,4 (AUTHOR), Rochette, Patrick J.1,5 (AUTHOR), Pouliot, Roxane1,2 (AUTHOR) roxane.pouliot@pha.ulaval.ca |
| Source: | Photochemistry & Photobiology. Sep/Oct2023, Vol. 99 Issue 5, p1258-1268. 11p. |
| Subjects: | Cigarette smoke, Toxicological interactions, Smoking, Solar radiation, Poisons |
| Abstract: | Solar radiation and cigarette smoke are two environmental risk factors known to affect skin integrity. Although the toxic effects of these factors on skin have been widely studied separately, few studies have focused on their interaction. The objective of this study was to evaluate and understand the synergistic harmful effects of cigarette smoke and solar rays on human primary keratinocytes. The keratinocytes were exposed to cigarette smoke extract (CSE) and then irradiated with a solar simulator light (SSL). The viability, as determined by measuring metabolic activity of skin cells, and the levels of global reactive oxygen species (ROS) were evaluated after exposure to CSE and SSL. The combination of 3% CSE with 29 kJ m−2 UVA caused a decrease of 81% in cell viability, while with 10% to 20% CSE, the cell viability was null. This phototoxicity was accompanied by an increase in singlet oxygen but a decrease in type I ROS when CSE and SSL were combined in vitro. Surprisingly, an increase in the CSE's total antioxidant capacity was also observed. These results suggest a synergy between the two environmental factors in their effect on skin cells, and more precisely a phototoxicity causing a drastic decrease in cell viability. [ABSTRACT FROM AUTHOR] |
| Copyright of Photochemistry & Photobiology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 172301854 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Toxic Interaction Between Solar Radiation and Cigarette Smoke on Primary Human Keratinocytes. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Grenier%2C+Alexe%22">Grenier, Alexe</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Morissette%2C+Mathieu+C%2E%22">Morissette, Mathieu C.</searchLink><relatesTo>3,4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Rochette%2C+Patrick+J%2E%22">Rochette, Patrick J.</searchLink><relatesTo>1,5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pouliot%2C+Roxane%22">Pouliot, Roxane</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> roxane.pouliot@pha.ulaval.ca</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Photochemistry+%26+Photobiology%22">Photochemistry & Photobiology</searchLink>. Sep/Oct2023, Vol. 99 Issue 5, p1258-1268. 11p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Cigarette+smoke%22">Cigarette smoke</searchLink><br /><searchLink fieldCode="DE" term="%22Toxicological+interactions%22">Toxicological interactions</searchLink><br /><searchLink fieldCode="DE" term="%22Smoking%22">Smoking</searchLink><br /><searchLink fieldCode="DE" term="%22Solar+radiation%22">Solar radiation</searchLink><br /><searchLink fieldCode="DE" term="%22Poisons%22">Poisons</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Solar radiation and cigarette smoke are two environmental risk factors known to affect skin integrity. Although the toxic effects of these factors on skin have been widely studied separately, few studies have focused on their interaction. The objective of this study was to evaluate and understand the synergistic harmful effects of cigarette smoke and solar rays on human primary keratinocytes. The keratinocytes were exposed to cigarette smoke extract (CSE) and then irradiated with a solar simulator light (SSL). The viability, as determined by measuring metabolic activity of skin cells, and the levels of global reactive oxygen species (ROS) were evaluated after exposure to CSE and SSL. The combination of 3% CSE with 29 kJ m−2 UVA caused a decrease of 81% in cell viability, while with 10% to 20% CSE, the cell viability was null. This phototoxicity was accompanied by an increase in singlet oxygen but a decrease in type I ROS when CSE and SSL were combined in vitro. Surprisingly, an increase in the CSE's total antioxidant capacity was also observed. These results suggest a synergy between the two environmental factors in their effect on skin cells, and more precisely a phototoxicity causing a drastic decrease in cell viability. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Photochemistry & Photobiology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1111/php.13768 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 1258 Subjects: – SubjectFull: Cigarette smoke Type: general – SubjectFull: Toxicological interactions Type: general – SubjectFull: Smoking Type: general – SubjectFull: Solar radiation Type: general – SubjectFull: Poisons Type: general Titles: – TitleFull: Toxic Interaction Between Solar Radiation and Cigarette Smoke on Primary Human Keratinocytes. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Grenier, Alexe – PersonEntity: Name: NameFull: Morissette, Mathieu C. – PersonEntity: Name: NameFull: Rochette, Patrick J. – PersonEntity: Name: NameFull: Pouliot, Roxane IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 09 Text: Sep/Oct2023 Type: published Y: 2023 Identifiers: – Type: issn-print Value: 00318655 Numbering: – Type: volume Value: 99 – Type: issue Value: 5 Titles: – TitleFull: Photochemistry & Photobiology Type: main |
| ResultId | 1 |
