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Systemic Administration of Porphyromonas Gingivalis Lipopolysaccharide Induces Glial Activation and Depressive-Like Behavior in Rats.

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Title: Systemic Administration of Porphyromonas Gingivalis Lipopolysaccharide Induces Glial Activation and Depressive-Like Behavior in Rats.
Authors: Mamunur, Rahman1,2, Sadayuki Hashioka3 hashioka@f2.dion.ne.jp, Azis, Ilhamuddin A.4,5, Jaya, Muhammad A.4,6, Jerin, Sultana J. F.1,2, Kaori Kimura-Kataoka1, Junko Fujihara1, Ken Inoue7, Masatoshi Inagaki2, Haruo Takeshita1
Source: Journal of Integrative Neuroscience. 2023, Vol. 22 Issue 5, p1-8. 8p. 1 Diagram, 3 Charts, 3 Graphs.
Subjects: Porphyromonas gingivalis, Periodontitis, Glial fibrillary acidic protein, Sprague Dawley rats, Hippocampus (Brain), Mental depression
Abstract: Background: Periodontitis is one of the most common chronic inflammatory disorders in adults. Although clinical studies have suggested a causal relationship between periodontitis and major depression (MD), the biological mechanisms by which periodontitis instigates MD are unknown. We investigated whether a systemic administration of lipopolysaccharide (LPS) from Porphyromonas gingivalis (Pg), a major Gram-negative pathogen of periodontitis, causes depressive-like behavior and glial activation in the hippocampus and the prefrontal cortex (PFC), which are MD-related brain regions. Materials and Methods: Eight-week-old male Sprague Dawley rats were randomly divided into a behavioral test group and an immunohistochemistry group. The rats in each group were further assigned to the sham injection (saline) and Porphyromonas gingivalis-lipopolysaccharide (Pg-LPS) injection protocols. The rats received an intraperitoneal injection of saline or Pg-LPS with gradually increasing doses (day 1: 0.5, day 2: 0.5, day 3: 0.75, day 4: 0.75, day 5: 1.0, day 6: 1.0, and day 7: 1.0 mg/kg of body weight) for seven consecutive days. After the systemic administration, the behavior test group underwent the forced swimming test (FST) and Y-maze test. For the immunohistochemistry group, we quantified the immunoreactivity for microglial Iba-1 (ionized calcium-binding adapter molecule 1) and astrocytic glial fibrillary acidic protein (GFAP) in the hippocampus (dentate gyrus [DG], cornu ammonis [CA1 and CA3]) and PFC (prelimbic [PrL] and the infralimbic [IL]) areas. Results: The FST immobility time in the Pg-LPS group was significantly longer than that in the sham group. In the Y-maze test, a significant decline in spontaneous alternation behavior was observed in the Pg-LPS group compared to the sham group. The peripheral administration of Pg-LPS significantly increased the immunoreactivity for Iba-1 in the CA3 and PrL. Pg-LPS injection significantly increased the immunoreactivity for GFAP in the DG, CA1, and CA3. Conclusions: The major result of this study is that a repeated systemic administration of Pg-LPS caused depressive-like behavior and both microglial and astrocytic activation in rats. This finding may comprise biological evidence of a causal relationship between periodontitis and MD. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Integrative Neuroscience is the property of IMR Press and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Label: Title
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  Data: Systemic Administration of Porphyromonas Gingivalis Lipopolysaccharide Induces Glial Activation and Depressive-Like Behavior in Rats.
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  Data: <searchLink fieldCode="AR" term="%22Mamunur%2C+Rahman%22">Mamunur, Rahman</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Sadayuki+Hashioka%22">Sadayuki Hashioka</searchLink><relatesTo>3</relatesTo><i> hashioka@f2.dion.ne.jp</i><br /><searchLink fieldCode="AR" term="%22Azis%2C+Ilhamuddin+A%2E%22">Azis, Ilhamuddin A.</searchLink><relatesTo>4,5</relatesTo><br /><searchLink fieldCode="AR" term="%22Jaya%2C+Muhammad+A%2E%22">Jaya, Muhammad A.</searchLink><relatesTo>4,6</relatesTo><br /><searchLink fieldCode="AR" term="%22Jerin%2C+Sultana+J%2E+F%2E%22">Jerin, Sultana J. F.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Kaori+Kimura-Kataoka%22">Kaori Kimura-Kataoka</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Junko+Fujihara%22">Junko Fujihara</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Ken+Inoue%22">Ken Inoue</searchLink><relatesTo>7</relatesTo><br /><searchLink fieldCode="AR" term="%22Masatoshi+Inagaki%22">Masatoshi Inagaki</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Haruo+Takeshita%22">Haruo Takeshita</searchLink><relatesTo>1</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Integrative+Neuroscience%22">Journal of Integrative Neuroscience</searchLink>. 2023, Vol. 22 Issue 5, p1-8. 8p. 1 Diagram, 3 Charts, 3 Graphs.
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  Data: <searchLink fieldCode="DE" term="%22Porphyromonas+gingivalis%22">Porphyromonas gingivalis</searchLink><br /><searchLink fieldCode="DE" term="%22Periodontitis%22">Periodontitis</searchLink><br /><searchLink fieldCode="DE" term="%22Glial+fibrillary+acidic+protein%22">Glial fibrillary acidic protein</searchLink><br /><searchLink fieldCode="DE" term="%22Sprague+Dawley+rats%22">Sprague Dawley rats</searchLink><br /><searchLink fieldCode="DE" term="%22Hippocampus+%28Brain%29%22">Hippocampus (Brain)</searchLink><br /><searchLink fieldCode="DE" term="%22Mental+depression%22">Mental depression</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Background: Periodontitis is one of the most common chronic inflammatory disorders in adults. Although clinical studies have suggested a causal relationship between periodontitis and major depression (MD), the biological mechanisms by which periodontitis instigates MD are unknown. We investigated whether a systemic administration of lipopolysaccharide (LPS) from Porphyromonas gingivalis (Pg), a major Gram-negative pathogen of periodontitis, causes depressive-like behavior and glial activation in the hippocampus and the prefrontal cortex (PFC), which are MD-related brain regions. Materials and Methods: Eight-week-old male Sprague Dawley rats were randomly divided into a behavioral test group and an immunohistochemistry group. The rats in each group were further assigned to the sham injection (saline) and Porphyromonas gingivalis-lipopolysaccharide (Pg-LPS) injection protocols. The rats received an intraperitoneal injection of saline or Pg-LPS with gradually increasing doses (day 1: 0.5, day 2: 0.5, day 3: 0.75, day 4: 0.75, day 5: 1.0, day 6: 1.0, and day 7: 1.0 mg/kg of body weight) for seven consecutive days. After the systemic administration, the behavior test group underwent the forced swimming test (FST) and Y-maze test. For the immunohistochemistry group, we quantified the immunoreactivity for microglial Iba-1 (ionized calcium-binding adapter molecule 1) and astrocytic glial fibrillary acidic protein (GFAP) in the hippocampus (dentate gyrus [DG], cornu ammonis [CA1 and CA3]) and PFC (prelimbic [PrL] and the infralimbic [IL]) areas. Results: The FST immobility time in the Pg-LPS group was significantly longer than that in the sham group. In the Y-maze test, a significant decline in spontaneous alternation behavior was observed in the Pg-LPS group compared to the sham group. The peripheral administration of Pg-LPS significantly increased the immunoreactivity for Iba-1 in the CA3 and PrL. Pg-LPS injection significantly increased the immunoreactivity for GFAP in the DG, CA1, and CA3. Conclusions: The major result of this study is that a repeated systemic administration of Pg-LPS caused depressive-like behavior and both microglial and astrocytic activation in rats. This finding may comprise biological evidence of a causal relationship between periodontitis and MD. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of Journal of Integrative Neuroscience is the property of IMR Press and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.31083/j.jin2205120
    Languages:
      – Code: eng
        Text: English
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      Pagination:
        PageCount: 8
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    Subjects:
      – SubjectFull: Porphyromonas gingivalis
        Type: general
      – SubjectFull: Periodontitis
        Type: general
      – SubjectFull: Glial fibrillary acidic protein
        Type: general
      – SubjectFull: Sprague Dawley rats
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      – SubjectFull: Hippocampus (Brain)
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      – SubjectFull: Mental depression
        Type: general
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      – TitleFull: Systemic Administration of Porphyromonas Gingivalis Lipopolysaccharide Induces Glial Activation and Depressive-Like Behavior in Rats.
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              M: 09
              Text: 2023
              Type: published
              Y: 2023
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