Characterization of the effects of exogenous abscisic acid (ABA) application on the expression of ABA-responsive genes in Abies koreana.
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| Title: | Characterization of the effects of exogenous abscisic acid (ABA) application on the expression of ABA-responsive genes in Abies koreana. |
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| Authors: | Lee, Da Young1 (AUTHOR), Kim, Dong Wook2 (AUTHOR), Park, Da Young1 (AUTHOR), Park, Jong-won1 (AUTHOR), Park, Hyeong Cheol1 (AUTHOR) hcpark@nie.re.kr |
| Source: | Plant Biotechnology Reports. Oct2023, Vol. 17 Issue 5, p777-785. 9p. |
| Subjects: | Gene expression, Genetic regulation, Fir, Pine needles, Abiotic stress, Plant species |
| Abstract: | Abies koreana, a plant species endemic to Korea, is sensitive to climate change. However, little is known about the molecular mechanisms underlying its environmental stress response. Here, we characterized the effects of exogenous abscisic acid (ABA) concentration, application method, and application duration on the expression of ABA-responsive genes in A. koreana. The expression level of AkNAC19 was significantly higher in ABA (0.2, 0.4, 0.8 mM)-treated leaves (needles) than in non-treated samples, indicating the role of ABA in the induction of gene expression. In addition, 0.2 mM ABA was found to be optimal for gene regulation. When assessing the efficacies of ABA application methods, the dipping method was slightly more effective in inducing gene expression than the pressure method. Therefore, leaves were further treated with 0.2 mM ABA using the dipping method to investigate the expression levels of multiple ABA-responsive genes, including AkNAC19, AkMPK6, AkERF4, AkNAC2, AkbHLH, AkEFP, AkHSP17.6, and AkMYB123. The expression levels of all genes, except AkMPK6, were significantly higher in ABA-treated leaves than in non-treated leaves. However, the optimal treatment duration for gene expression induction varied with the gene type. Overall, these results suggest that A. koreana responds to abiotic stress by upregulating the expression of ABA-responsive genes. [ABSTRACT FROM AUTHOR] |
| Copyright of Plant Biotechnology Reports is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 173273452 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Characterization of the effects of exogenous abscisic acid (ABA) application on the expression of ABA-responsive genes in Abies koreana. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Lee%2C+Da+Young%22">Lee, Da Young</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kim%2C+Dong+Wook%22">Kim, Dong Wook</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Park%2C+Da+Young%22">Park, Da Young</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Park%2C+Jong-won%22">Park, Jong-won</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Park%2C+Hyeong+Cheol%22">Park, Hyeong Cheol</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> hcpark@nie.re.kr</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Plant+Biotechnology+Reports%22">Plant Biotechnology Reports</searchLink>. Oct2023, Vol. 17 Issue 5, p777-785. 9p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+regulation%22">Genetic regulation</searchLink><br /><searchLink fieldCode="DE" term="%22Fir%22">Fir</searchLink><br /><searchLink fieldCode="DE" term="%22Pine+needles%22">Pine needles</searchLink><br /><searchLink fieldCode="DE" term="%22Abiotic+stress%22">Abiotic stress</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+species%22">Plant species</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Abies koreana, a plant species endemic to Korea, is sensitive to climate change. However, little is known about the molecular mechanisms underlying its environmental stress response. Here, we characterized the effects of exogenous abscisic acid (ABA) concentration, application method, and application duration on the expression of ABA-responsive genes in A. koreana. The expression level of AkNAC19 was significantly higher in ABA (0.2, 0.4, 0.8 mM)-treated leaves (needles) than in non-treated samples, indicating the role of ABA in the induction of gene expression. In addition, 0.2 mM ABA was found to be optimal for gene regulation. When assessing the efficacies of ABA application methods, the dipping method was slightly more effective in inducing gene expression than the pressure method. Therefore, leaves were further treated with 0.2 mM ABA using the dipping method to investigate the expression levels of multiple ABA-responsive genes, including AkNAC19, AkMPK6, AkERF4, AkNAC2, AkbHLH, AkEFP, AkHSP17.6, and AkMYB123. The expression levels of all genes, except AkMPK6, were significantly higher in ABA-treated leaves than in non-treated leaves. However, the optimal treatment duration for gene expression induction varied with the gene type. Overall, these results suggest that A. koreana responds to abiotic stress by upregulating the expression of ABA-responsive genes. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Plant Biotechnology Reports is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s11816-023-00852-7 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 777 Subjects: – SubjectFull: Gene expression Type: general – SubjectFull: Genetic regulation Type: general – SubjectFull: Fir Type: general – SubjectFull: Pine needles Type: general – SubjectFull: Abiotic stress Type: general – SubjectFull: Plant species Type: general Titles: – TitleFull: Characterization of the effects of exogenous abscisic acid (ABA) application on the expression of ABA-responsive genes in Abies koreana. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Lee, Da Young – PersonEntity: Name: NameFull: Kim, Dong Wook – PersonEntity: Name: NameFull: Park, Da Young – PersonEntity: Name: NameFull: Park, Jong-won – PersonEntity: Name: NameFull: Park, Hyeong Cheol IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 10 Text: Oct2023 Type: published Y: 2023 Identifiers: – Type: issn-print Value: 18635466 Numbering: – Type: volume Value: 17 – Type: issue Value: 5 Titles: – TitleFull: Plant Biotechnology Reports Type: main |
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