Characterization of the effects of exogenous abscisic acid (ABA) application on the expression of ABA-responsive genes in Abies koreana.
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| Title: | Characterization of the effects of exogenous abscisic acid (ABA) application on the expression of ABA-responsive genes in Abies koreana. |
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| Authors: | Lee, Da Young1 (AUTHOR), Kim, Dong Wook2 (AUTHOR), Park, Da Young1 (AUTHOR), Park, Jong-won1 (AUTHOR), Park, Hyeong Cheol1 (AUTHOR) hcpark@nie.re.kr |
| Source: | Plant Biotechnology Reports. Oct2023, Vol. 17 Issue 5, p777-785. 9p. |
| Subjects: | Gene expression, Genetic regulation, Fir, Pine needles, Abiotic stress, Plant species |
| Abstract: | Abies koreana, a plant species endemic to Korea, is sensitive to climate change. However, little is known about the molecular mechanisms underlying its environmental stress response. Here, we characterized the effects of exogenous abscisic acid (ABA) concentration, application method, and application duration on the expression of ABA-responsive genes in A. koreana. The expression level of AkNAC19 was significantly higher in ABA (0.2, 0.4, 0.8 mM)-treated leaves (needles) than in non-treated samples, indicating the role of ABA in the induction of gene expression. In addition, 0.2 mM ABA was found to be optimal for gene regulation. When assessing the efficacies of ABA application methods, the dipping method was slightly more effective in inducing gene expression than the pressure method. Therefore, leaves were further treated with 0.2 mM ABA using the dipping method to investigate the expression levels of multiple ABA-responsive genes, including AkNAC19, AkMPK6, AkERF4, AkNAC2, AkbHLH, AkEFP, AkHSP17.6, and AkMYB123. The expression levels of all genes, except AkMPK6, were significantly higher in ABA-treated leaves than in non-treated leaves. However, the optimal treatment duration for gene expression induction varied with the gene type. Overall, these results suggest that A. koreana responds to abiotic stress by upregulating the expression of ABA-responsive genes. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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