The effects of antimicrobial photodynamic therapy (aPDT) with nanotechnology-applied curcumin and 450nm blue led irradiation on multi-species biofilms in root canals.
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| Title: | The effects of antimicrobial photodynamic therapy (aPDT) with nanotechnology-applied curcumin and 450nm blue led irradiation on multi-species biofilms in root canals. |
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| Authors: | Ravazzi, Thayse Pithon Quadros1 (AUTHOR) thayse.pithon@gmail.com, de Jesus, Iasmym Mendes1 (AUTHOR), de Oliveira Santos, Gabriel Pinto1 (AUTHOR), Reis, Thaís Azevedo1 (AUTHOR), Rosa, Luciano Pereira1 (AUTHOR), Rosa, Francine Cristina Silva1 (AUTHOR) |
| Source: | Lasers in Medical Science. 11/7/2023, Vol. 38 Issue 1, p1-9. 9p. |
| Subjects: | Dental pulp cavities, Photodynamic therapy, Curcumin, Methicillin-resistant staphylococcus aureus, Biofilms |
| Abstract: | This study aimed to evaluate the effectiveness of antimicrobial photodynamic therapy (aPDT) utilizing nanotechnology-applied curcumin activated by blue LED (450 nm) on the elimination of microorganisms arranged in multispecies biofilms inside the root canals of extracted human teeth. Forty single-rooted human teeth were used; these were randomized into four experimental groups, each comprising 10 teeth: control group, no treatment; photosensitizer (PS) group, nanotechnology-applied curcumin alone; light group, blue LED used separately; and aPDT group, nanotechnology-applied curcumin activated by blue LED. To carry out the tests, the interiors of the root canals were inoculated with species of Candida albicans (ATCC 90029), Enterococcus faecalis (ATCC 29212), Escherichia coli (ATCC 25922), and methicillin-resistant Staphylococcus aureus (MRSA) (ATCC 43300), using a multispecies biofilm. After the incubation period, the canals were treated according to the experimental groups, with no treatment given in the control group. Studied inasmuch as the antimicrobial effectiveness of aPDT was concerned, it was observed that the greatest reduction in microbial counts using aPDTs was achieved against MRSA (mean reduction = 2.48 Log10 CFU/mL), followed by Escherichia coli (mean reduction = 1.72), and Enterococcus faecalis (mean reduction = 1.65); a reduction greater than 1.5 Log10 CFU/mL showed relevant effectiveness of aPDT against these microorganisms. Of note, aPDT has also shown considerable effectiveness against Candida albicans (mean reduction = 0.71), with a statistical difference in the reduction between the groups. aPDT was effective in reducing all microorganisms examined. The average reduction was greater than 1.5 Log10 in all microorganisms except for Candida albicans. Highlights: • aPDT was a viable treatment for root canals; • Nanotechnological curcumin aPDT was effective in reducing multispecies biofilm microorganisms; • aPDT technique showed efficacy under the worst microbiological conditions , such as mature multispecies biofilm; • Nanotechnological curcumin aPDT was able to reduce Gram positive, negative bacterial and yeasts in root canals. [ABSTRACT FROM AUTHOR] |
| Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Header | DbId: egs DbLabel: Engineering Source An: 173457384 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: The effects of antimicrobial photodynamic therapy (aPDT) with nanotechnology-applied curcumin and 450nm blue led irradiation on multi-species biofilms in root canals. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Ravazzi%2C+Thayse+Pithon+Quadros%22">Ravazzi, Thayse Pithon Quadros</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> thayse.pithon@gmail.com</i><br /><searchLink fieldCode="AR" term="%22de+Jesus%2C+Iasmym+Mendes%22">de Jesus, Iasmym Mendes</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22de+Oliveira+Santos%2C+Gabriel+Pinto%22">de Oliveira Santos, Gabriel Pinto</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Reis%2C+Thaís+Azevedo%22">Reis, Thaís Azevedo</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Rosa%2C+Luciano+Pereira%22">Rosa, Luciano Pereira</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Rosa%2C+Francine+Cristina+Silva%22">Rosa, Francine Cristina Silva</searchLink><relatesTo>1</relatesTo> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Lasers+in+Medical+Science%22">Lasers in Medical Science</searchLink>. 11/7/2023, Vol. 38 Issue 1, p1-9. 9p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Dental+pulp+cavities%22">Dental pulp cavities</searchLink><br /><searchLink fieldCode="DE" term="%22Photodynamic+therapy%22">Photodynamic therapy</searchLink><br /><searchLink fieldCode="DE" term="%22Curcumin%22">Curcumin</searchLink><br /><searchLink fieldCode="DE" term="%22Methicillin-resistant+staphylococcus+aureus%22">Methicillin-resistant staphylococcus aureus</searchLink><br /><searchLink fieldCode="DE" term="%22Biofilms%22">Biofilms</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: This study aimed to evaluate the effectiveness of antimicrobial photodynamic therapy (aPDT) utilizing nanotechnology-applied curcumin activated by blue LED (450 nm) on the elimination of microorganisms arranged in multispecies biofilms inside the root canals of extracted human teeth. Forty single-rooted human teeth were used; these were randomized into four experimental groups, each comprising 10 teeth: control group, no treatment; photosensitizer (PS) group, nanotechnology-applied curcumin alone; light group, blue LED used separately; and aPDT group, nanotechnology-applied curcumin activated by blue LED. To carry out the tests, the interiors of the root canals were inoculated with species of Candida albicans (ATCC 90029), Enterococcus faecalis (ATCC 29212), Escherichia coli (ATCC 25922), and methicillin-resistant Staphylococcus aureus (MRSA) (ATCC 43300), using a multispecies biofilm. After the incubation period, the canals were treated according to the experimental groups, with no treatment given in the control group. Studied inasmuch as the antimicrobial effectiveness of aPDT was concerned, it was observed that the greatest reduction in microbial counts using aPDTs was achieved against MRSA (mean reduction = 2.48 Log10 CFU/mL), followed by Escherichia coli (mean reduction = 1.72), and Enterococcus faecalis (mean reduction = 1.65); a reduction greater than 1.5 Log10 CFU/mL showed relevant effectiveness of aPDT against these microorganisms. Of note, aPDT has also shown considerable effectiveness against Candida albicans (mean reduction = 0.71), with a statistical difference in the reduction between the groups. aPDT was effective in reducing all microorganisms examined. The average reduction was greater than 1.5 Log10 in all microorganisms except for Candida albicans. Highlights: • aPDT was a viable treatment for root canals; • Nanotechnological curcumin aPDT was effective in reducing multispecies biofilm microorganisms; • aPDT technique showed efficacy under the worst microbiological conditions , such as mature multispecies biofilm; • Nanotechnological curcumin aPDT was able to reduce Gram positive, negative bacterial and yeasts in root canals. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=173457384 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s10103-023-03925-9 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 1 Subjects: – SubjectFull: Dental pulp cavities Type: general – SubjectFull: Photodynamic therapy Type: general – SubjectFull: Curcumin Type: general – SubjectFull: Methicillin-resistant staphylococcus aureus Type: general – SubjectFull: Biofilms Type: general Titles: – TitleFull: The effects of antimicrobial photodynamic therapy (aPDT) with nanotechnology-applied curcumin and 450nm blue led irradiation on multi-species biofilms in root canals. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Ravazzi, Thayse Pithon Quadros – PersonEntity: Name: NameFull: de Jesus, Iasmym Mendes – PersonEntity: Name: NameFull: de Oliveira Santos, Gabriel Pinto – PersonEntity: Name: NameFull: Reis, Thaís Azevedo – PersonEntity: Name: NameFull: Rosa, Luciano Pereira – PersonEntity: Name: NameFull: Rosa, Francine Cristina Silva IsPartOfRelationships: – BibEntity: Dates: – D: 07 M: 11 Text: 11/7/2023 Type: published Y: 2023 Identifiers: – Type: issn-print Value: 02688921 Numbering: – Type: volume Value: 38 – Type: issue Value: 1 Titles: – TitleFull: Lasers in Medical Science Type: main |
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