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Cancer Marker Immunosensing through Surface-Enhanced Photoluminescence on Nanostructured Silver Substrates.

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Title: Cancer Marker Immunosensing through Surface-Enhanced Photoluminescence on Nanostructured Silver Substrates.
Authors: Geka, Georgia1,2 (AUTHOR) g.gkeka@rrp.demokritos.gr, Kanioura, Anastasia1 (AUTHOR) nkanioura@ipta.demokritos.gr, Kochylas, Ioannis3 (AUTHOR) ikochyla@phys.uoa.gr, Likodimos, Vlassis3 (AUTHOR) vlikodimos@phys.uoa.gr, Gardelis, Spiros3 (AUTHOR) sgardelis@phys.uoa.gr, Dimitriou, Anastasios4 (AUTHOR) a.dimitriou@inn.demokritos.gr, Papanikolaou, Nikolaos4 (AUTHOR) n.papanikolaou@inn.demokritos.gr, Chatzantonaki, Kalliopi5 (AUTHOR) p.chatzantonaki@invitrolabs.gr, Charvalos, Ekaterina5 (AUTHOR) directorcentralabs@iaso.gr, Economou, Anastasios2 (AUTHOR), Kakabakos, Sotirios1 (AUTHOR) skakab@rrp.demokritos.gr, Petrou, Panagiota1 (AUTHOR) ypetrou@rrp.demokritos.gr
Source: Nanomaterials (2079-4991). Dec2023, Vol. 13 Issue 24, p3099. 14p.
Subjects: Tumor markers, Silicon nanowires, Photoluminescence, Streptavidin, Precious metals, Silver nanoparticles, Silver
Abstract: Nanostructured noble metal surfaces enhance the photoluminescence emitted by fluorescent molecules, permitting the development of highly sensitive fluorescence immunoassays. To this end, surfaces with silicon nanowires decorated with silver nanoparticles in the form of dendrites or aggregates were evaluated as substrates for the immunochemical detection of two ovarian cancer indicators, carbohydrate antigen 125 (CA125) and human epididymis protein 4 (HE4). The substrates were prepared by metal-enhanced chemical etching of silicon wafers to create, in one step, silicon nanowires and silver nanoparticles on top of them. For both analytes, non-competitive immunoassays were developed using pairs of highly specific monoclonal antibodies, one for analyte capture on the substrate and the other for detection. In order to facilitate the identification of the immunocomplexes through a reaction with streptavidin labeled with Rhodamine Red-X, the detection antibodies were biotinylated. An in-house-developed optical set-up was used for photoluminescence signal measurements after assay completion. The detection limits achieved were 2.5 U/mL and 3.12 pM for CA125 and HE4, respectively, with linear dynamic ranges extending up to 500 U/mL for CA125 and up to 500 pM for HE4, covering the concentration ranges of both healthy and ovarian cancer patients. Thus, the proposed method could be implemented for the early diagnosis and/or prognosis and monitoring of ovarian cancer. [ABSTRACT FROM AUTHOR]
Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Label: Title
  Group: Ti
  Data: Cancer Marker Immunosensing through Surface-Enhanced Photoluminescence on Nanostructured Silver Substrates.
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  Data: <searchLink fieldCode="AR" term="%22Geka%2C+Georgia%22">Geka, Georgia</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> g.gkeka@rrp.demokritos.gr</i><br /><searchLink fieldCode="AR" term="%22Kanioura%2C+Anastasia%22">Kanioura, Anastasia</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> nkanioura@ipta.demokritos.gr</i><br /><searchLink fieldCode="AR" term="%22Kochylas%2C+Ioannis%22">Kochylas, Ioannis</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> ikochyla@phys.uoa.gr</i><br /><searchLink fieldCode="AR" term="%22Likodimos%2C+Vlassis%22">Likodimos, Vlassis</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> vlikodimos@phys.uoa.gr</i><br /><searchLink fieldCode="AR" term="%22Gardelis%2C+Spiros%22">Gardelis, Spiros</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> sgardelis@phys.uoa.gr</i><br /><searchLink fieldCode="AR" term="%22Dimitriou%2C+Anastasios%22">Dimitriou, Anastasios</searchLink><relatesTo>4</relatesTo> (AUTHOR)<i> a.dimitriou@inn.demokritos.gr</i><br /><searchLink fieldCode="AR" term="%22Papanikolaou%2C+Nikolaos%22">Papanikolaou, Nikolaos</searchLink><relatesTo>4</relatesTo> (AUTHOR)<i> n.papanikolaou@inn.demokritos.gr</i><br /><searchLink fieldCode="AR" term="%22Chatzantonaki%2C+Kalliopi%22">Chatzantonaki, Kalliopi</searchLink><relatesTo>5</relatesTo> (AUTHOR)<i> p.chatzantonaki@invitrolabs.gr</i><br /><searchLink fieldCode="AR" term="%22Charvalos%2C+Ekaterina%22">Charvalos, Ekaterina</searchLink><relatesTo>5</relatesTo> (AUTHOR)<i> directorcentralabs@iaso.gr</i><br /><searchLink fieldCode="AR" term="%22Economou%2C+Anastasios%22">Economou, Anastasios</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kakabakos%2C+Sotirios%22">Kakabakos, Sotirios</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> skakab@rrp.demokritos.gr</i><br /><searchLink fieldCode="AR" term="%22Petrou%2C+Panagiota%22">Petrou, Panagiota</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> ypetrou@rrp.demokritos.gr</i>
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  Data: <searchLink fieldCode="JN" term="%22Nanomaterials+%282079-4991%29%22">Nanomaterials (2079-4991)</searchLink>. Dec2023, Vol. 13 Issue 24, p3099. 14p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Tumor+markers%22">Tumor markers</searchLink><br /><searchLink fieldCode="DE" term="%22Silicon+nanowires%22">Silicon nanowires</searchLink><br /><searchLink fieldCode="DE" term="%22Photoluminescence%22">Photoluminescence</searchLink><br /><searchLink fieldCode="DE" term="%22Streptavidin%22">Streptavidin</searchLink><br /><searchLink fieldCode="DE" term="%22Precious+metals%22">Precious metals</searchLink><br /><searchLink fieldCode="DE" term="%22Silver+nanoparticles%22">Silver nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Silver%22">Silver</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Nanostructured noble metal surfaces enhance the photoluminescence emitted by fluorescent molecules, permitting the development of highly sensitive fluorescence immunoassays. To this end, surfaces with silicon nanowires decorated with silver nanoparticles in the form of dendrites or aggregates were evaluated as substrates for the immunochemical detection of two ovarian cancer indicators, carbohydrate antigen 125 (CA125) and human epididymis protein 4 (HE4). The substrates were prepared by metal-enhanced chemical etching of silicon wafers to create, in one step, silicon nanowires and silver nanoparticles on top of them. For both analytes, non-competitive immunoassays were developed using pairs of highly specific monoclonal antibodies, one for analyte capture on the substrate and the other for detection. In order to facilitate the identification of the immunocomplexes through a reaction with streptavidin labeled with Rhodamine Red-X, the detection antibodies were biotinylated. An in-house-developed optical set-up was used for photoluminescence signal measurements after assay completion. The detection limits achieved were 2.5 U/mL and 3.12 pM for CA125 and HE4, respectively, with linear dynamic ranges extending up to 500 U/mL for CA125 and up to 500 pM for HE4, covering the concentration ranges of both healthy and ovarian cancer patients. Thus, the proposed method could be implemented for the early diagnosis and/or prognosis and monitoring of ovarian cancer. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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    Identifiers:
      – Type: doi
        Value: 10.3390/nano13243099
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      – Code: eng
        Text: English
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        PageCount: 14
        StartPage: 3099
    Subjects:
      – SubjectFull: Tumor markers
        Type: general
      – SubjectFull: Silicon nanowires
        Type: general
      – SubjectFull: Photoluminescence
        Type: general
      – SubjectFull: Streptavidin
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      – SubjectFull: Precious metals
        Type: general
      – SubjectFull: Silver nanoparticles
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      – SubjectFull: Silver
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      – TitleFull: Cancer Marker Immunosensing through Surface-Enhanced Photoluminescence on Nanostructured Silver Substrates.
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              Text: Dec2023
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