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Binding of JAB1/CSN5 to MIF is mediated by the MPN domain but is independent of the JAMM motif

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Title: Binding of JAB1/CSN5 to MIF is mediated by the MPN domain but is independent of the JAMM motif
Authors: Burger-Kentischer, Anke1, Finkelmeier, Doris1, Thiele, Michael1, Schmucker, Jürgen1, Geiger, Georg1, Tovar, Günter E.M.1,2, Bernhagen, Jürgen1 jbernhagen@ukaachen.de
Source: FEBS Letters. Mar2005, Vol. 579 Issue 7, p1693-1701. 9p.
Subjects: Carrier proteins, Blood plasma, Cellular immunity, Macrophages
Abstract: Abstract: Macrophage migration inhibitory factor (MIF) binds to c-Jun activation domain binding protein-1 (JAB1)/subunit 5 of COP9 signalosome (CSN5) and modulates cell signaling and the cell cycle through JAB1. The binding domain of JAB1 responsible for binding to MIF is unknown. We hypothesized that the conserved Mpr1p Pad1p N-terminal (MPN) domain of JAB1 may mediate binding to MIF. In fact, yeast two hybrid (YTH) and in vitro translation/coimmunoprecipitation (CoIP) analysis showed that a core MPN domain, which did not cover the functional JAB1/MPN/Mov34 metalloenzyme (JAMM) deneddylase sequence, binds to MIF comparable to full-length JAB1. YTH and pull-down analysis in conjunction with nanobead affinity matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry demonstrated that MIF(50–65) and MPN are sufficient to mediate MIF–JAB1 interaction, respectively. Finally, endogenous CoIP of MIF–CSN6 complexes from mammalian cells demonstrated that MPN is responsible for MIF–JAB1 binding in vivo, and, as CSN6 does not contain a functional JAMM motif, confirmed that the interaction does not require JAMM. [Copyright &y& Elsevier]
Copyright of FEBS Letters is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 17464955
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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Items – Name: Title
  Label: Title
  Group: Ti
  Data: Binding of JAB1/CSN5 to MIF is mediated by the MPN domain but is independent of the JAMM motif
– Name: Author
  Label: Authors
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  Data: <searchLink fieldCode="AR" term="%22Burger-Kentischer%2C+Anke%22">Burger-Kentischer, Anke</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Finkelmeier%2C+Doris%22">Finkelmeier, Doris</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Thiele%2C+Michael%22">Thiele, Michael</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Schmucker%2C+Jürgen%22">Schmucker, Jürgen</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Geiger%2C+Georg%22">Geiger, Georg</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Tovar%2C+Günter+E%2EM%2E%22">Tovar, Günter E.M.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Bernhagen%2C+Jürgen%22">Bernhagen, Jürgen</searchLink><relatesTo>1</relatesTo><i> jbernhagen@ukaachen.de</i>
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  Data: <searchLink fieldCode="JN" term="%22FEBS+Letters%22">FEBS Letters</searchLink>. Mar2005, Vol. 579 Issue 7, p1693-1701. 9p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Carrier+proteins%22">Carrier proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Blood+plasma%22">Blood plasma</searchLink><br /><searchLink fieldCode="DE" term="%22Cellular+immunity%22">Cellular immunity</searchLink><br /><searchLink fieldCode="DE" term="%22Macrophages%22">Macrophages</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Abstract: Macrophage migration inhibitory factor (MIF) binds to c-Jun activation domain binding protein-1 (JAB1)/subunit 5 of COP9 signalosome (CSN5) and modulates cell signaling and the cell cycle through JAB1. The binding domain of JAB1 responsible for binding to MIF is unknown. We hypothesized that the conserved Mpr1p Pad1p N-terminal (MPN) domain of JAB1 may mediate binding to MIF. In fact, yeast two hybrid (YTH) and in vitro translation/coimmunoprecipitation (CoIP) analysis showed that a core MPN domain, which did not cover the functional JAB1/MPN/Mov34 metalloenzyme (JAMM) deneddylase sequence, binds to MIF comparable to full-length JAB1. YTH and pull-down analysis in conjunction with nanobead affinity matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry demonstrated that MIF(50–65) and MPN are sufficient to mediate MIF–JAB1 interaction, respectively. Finally, endogenous CoIP of MIF–CSN6 complexes from mammalian cells demonstrated that MPN is responsible for MIF–JAB1 binding in vivo, and, as CSN6 does not contain a functional JAMM motif, confirmed that the interaction does not require JAMM. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of FEBS Letters is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.febslet.2005.01.080
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      – Code: eng
        Text: English
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        PageCount: 9
        StartPage: 1693
    Subjects:
      – SubjectFull: Carrier proteins
        Type: general
      – SubjectFull: Blood plasma
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      – SubjectFull: Cellular immunity
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      – SubjectFull: Macrophages
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      – TitleFull: Binding of JAB1/CSN5 to MIF is mediated by the MPN domain but is independent of the JAMM motif
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              Text: Mar2005
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