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Smartphone-assisted ratiometric fluorescence sensor for sensitive and portable α-glucosidase activity detection and inhibitor screening.

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Title: Smartphone-assisted ratiometric fluorescence sensor for sensitive and portable α-glucosidase activity detection and inhibitor screening.
Authors: Cao, Hui-Ting1 (AUTHOR), Zhao, Tiansheng1 (AUTHOR), Liu, Wei2 (AUTHOR), Xu, Cai-Ying1 (AUTHOR), Liao, Yi-Jing1 (AUTHOR), Yan, Xi-Luan1 (AUTHOR), Mai, Xi1 (AUTHOR) maixi123@aliyun.com, Li, Na1 (AUTHOR) lin@ncu.edu.cn
Source: Microchemical Journal. Feb2024, Vol. 197, pN.PAG-N.PAG. 1p.
Subjects: Medical screening, Fluorescence, Resource-limited settings, Fluorescence quenching, Alpha-glucosidases, High throughput screening (Drug development), Smartphones
Abstract: [Display omitted] • A portable smartphone-assisted sensor is developed for rapid α-glucosidase detection. • The proposed method is highly sensitive with LOD of 0.184 mU/mL α-glucosidase. • The in-situ synthesis of polymeric carbon dots is mild, time-saving, and economic. • The method can be applied to screening potential antidiabetic drugs from natural products. α-Glucosidase (α-Glu) is an enzyme that plays an essential role in the digestion of carbohydrates, and its activity detection and inhibitor screening are crucial for the therapy of diseases and development of new drugs. In this study, α-Glu can catalyze the hydrolysis of α-arbutin to produce hydroquinone (HQ), and HQ can regulate the in-situ synthesis of polymer carbon dots (PCDs) with ethylenediamine (EDA). PCDs and tetraphenylporphyrin tetrasulphonic acid (TPPS) are separately designed as green and red fluorescent sensing units. With the increase of α-Glu concentration, the number of PCDs is gradually increased, resulting in enhancement of green fluorescence and quenching of red fluorescence. Conversely, when α-Glu inhibitors (AGIs) are present, the activity of α-Glu is inhibited, leading to weak green fluorescence and strong red fluorescence. Thus a ratiometric fluorescence sensor was designed for the determination of α-Glu activity and screening of AGIs. The developed ratiometric sensor exhibits a linear relationship between fluorescence intensity ratios and α-Glu concentrations (0.01 to 0.04 U/mL) with limit of detection of 0.184 mU/mL, which is much lower than the single fluorescence model. In addition, rapid and portable detection of α-Glu can be achieved using a smartphone with a color picking software installed as a signal detection device, which greatly reduces the cost and time of detection and provides a promising method for qualitative identification and quantitative analysis of α-Glu in the resource-limited areas. Moreover, the method is very promising for high-throughput screening AGIs from natural products. [ABSTRACT FROM AUTHOR]
Copyright of Microchemical Journal is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 174842502
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  Label: Title
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  Data: Smartphone-assisted ratiometric fluorescence sensor for sensitive and portable α-glucosidase activity detection and inhibitor screening.
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  Data: <searchLink fieldCode="AR" term="%22Cao%2C+Hui-Ting%22">Cao, Hui-Ting</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhao%2C+Tiansheng%22">Zhao, Tiansheng</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Liu%2C+Wei%22">Liu, Wei</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Xu%2C+Cai-Ying%22">Xu, Cai-Ying</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Liao%2C+Yi-Jing%22">Liao, Yi-Jing</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yan%2C+Xi-Luan%22">Yan, Xi-Luan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mai%2C+Xi%22">Mai, Xi</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> maixi123@aliyun.com</i><br /><searchLink fieldCode="AR" term="%22Li%2C+Na%22">Li, Na</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> lin@ncu.edu.cn</i>
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  Data: <searchLink fieldCode="JN" term="%22Microchemical+Journal%22">Microchemical Journal</searchLink>. Feb2024, Vol. 197, pN.PAG-N.PAG. 1p.
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– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: [Display omitted] • A portable smartphone-assisted sensor is developed for rapid α-glucosidase detection. • The proposed method is highly sensitive with LOD of 0.184 mU/mL α-glucosidase. • The in-situ synthesis of polymeric carbon dots is mild, time-saving, and economic. • The method can be applied to screening potential antidiabetic drugs from natural products. α-Glucosidase (α-Glu) is an enzyme that plays an essential role in the digestion of carbohydrates, and its activity detection and inhibitor screening are crucial for the therapy of diseases and development of new drugs. In this study, α-Glu can catalyze the hydrolysis of α-arbutin to produce hydroquinone (HQ), and HQ can regulate the in-situ synthesis of polymer carbon dots (PCDs) with ethylenediamine (EDA). PCDs and tetraphenylporphyrin tetrasulphonic acid (TPPS) are separately designed as green and red fluorescent sensing units. With the increase of α-Glu concentration, the number of PCDs is gradually increased, resulting in enhancement of green fluorescence and quenching of red fluorescence. Conversely, when α-Glu inhibitors (AGIs) are present, the activity of α-Glu is inhibited, leading to weak green fluorescence and strong red fluorescence. Thus a ratiometric fluorescence sensor was designed for the determination of α-Glu activity and screening of AGIs. The developed ratiometric sensor exhibits a linear relationship between fluorescence intensity ratios and α-Glu concentrations (0.01 to 0.04 U/mL) with limit of detection of 0.184 mU/mL, which is much lower than the single fluorescence model. In addition, rapid and portable detection of α-Glu can be achieved using a smartphone with a color picking software installed as a signal detection device, which greatly reduces the cost and time of detection and provides a promising method for qualitative identification and quantitative analysis of α-Glu in the resource-limited areas. Moreover, the method is very promising for high-throughput screening AGIs from natural products. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Microchemical Journal is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.microc.2023.109723
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Medical screening
        Type: general
      – SubjectFull: Fluorescence
        Type: general
      – SubjectFull: Resource-limited settings
        Type: general
      – SubjectFull: Fluorescence quenching
        Type: general
      – SubjectFull: Alpha-glucosidases
        Type: general
      – SubjectFull: High throughput screening (Drug development)
        Type: general
      – SubjectFull: Smartphones
        Type: general
    Titles:
      – TitleFull: Smartphone-assisted ratiometric fluorescence sensor for sensitive and portable α-glucosidase activity detection and inhibitor screening.
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            NameFull: Cao, Hui-Ting
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            NameFull: Zhao, Tiansheng
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            NameFull: Liu, Wei
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            NameFull: Xu, Cai-Ying
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            NameFull: Yan, Xi-Luan
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            NameFull: Mai, Xi
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            – D: 01
              M: 02
              Text: Feb2024
              Type: published
              Y: 2024
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