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Using dynamic biomaterials to study the temporal role of bioactive peptides during osteogenesis.

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Title: Using dynamic biomaterials to study the temporal role of bioactive peptides during osteogenesis.
Authors: Fumasi, Fallon M.1 (AUTHOR), MacCulloch, Tara1,2,3 (AUTHOR), Bernal-Chanchavac, Julio1,2,3 (AUTHOR) jcbernal@asu.edu, Stephanopoulos, Nicholas1,2,3 (AUTHOR) nstepha1@asu.edu, Holloway, Julianne L.1,2 (AUTHOR) Julianne.Holloway@asu.edu
Source: Biomaterials Advances. Feb2024, Vol. 157, pN.PAG-N.PAG. 1p.
Subjects: Bone growth, Peptides, Biomaterials, Hyaluronic acid, Alkaline phosphatase, Cell adhesion molecules
Abstract: The extracellular matrix is a highly dynamic environment, and the precise temporal presentation of biochemical signals is critical for regulating cell behavior during development, healing, and disease progression. To mimic this behavior, we developed a modular DNA-based hydrogel platform to enable independent and reversible control over the immobilization of multiple biomolecules during in vitro cell culture. We combined reversible DNA handles with a norbornene-modified hyaluronic acid hydrogel to orthogonally add and remove multiple biomolecule-DNA conjugates at user-defined timepoints. We demonstrated that the persistent presentation of the cell adhesion peptide RGD was required to maintain cell spreading on hyaluronic acid hydrogels. Further, we discovered the delayed presentation of osteogenic growth peptide (OGP) increased alkaline phosphatase activity compared to other temporal variations. This finding is critically important when considering the design of OGP delivery approaches for bone repair. More broadly, this platform provides a unique approach to tease apart the temporal role of multiple biomolecules during development, regeneration, and disease progression. [Display omitted] • DNA handles enabled orthogonal and reversible control over peptide immobilization. • Persistent immobilization of RGD was required to maintain cell spreading. • The delayed immobilization of osteogenic growth peptide increases osteogenesis. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:The extracellular matrix is a highly dynamic environment, and the precise temporal presentation of biochemical signals is critical for regulating cell behavior during development, healing, and disease progression. To mimic this behavior, we developed a modular DNA-based hydrogel platform to enable independent and reversible control over the immobilization of multiple biomolecules during in vitro cell culture. We combined reversible DNA handles with a norbornene-modified hyaluronic acid hydrogel to orthogonally add and remove multiple biomolecule-DNA conjugates at user-defined timepoints. We demonstrated that the persistent presentation of the cell adhesion peptide RGD was required to maintain cell spreading on hyaluronic acid hydrogels. Further, we discovered the delayed presentation of osteogenic growth peptide (OGP) increased alkaline phosphatase activity compared to other temporal variations. This finding is critically important when considering the design of OGP delivery approaches for bone repair. More broadly, this platform provides a unique approach to tease apart the temporal role of multiple biomolecules during development, regeneration, and disease progression. [Display omitted] • DNA handles enabled orthogonal and reversible control over peptide immobilization. • Persistent immobilization of RGD was required to maintain cell spreading. • The delayed immobilization of osteogenic growth peptide increases osteogenesis. [ABSTRACT FROM AUTHOR]
ISSN:27729516
DOI:10.1016/j.bioadv.2023.213726