View in EDS

Using dynamic biomaterials to study the temporal role of bioactive peptides during osteogenesis.

Saved in:
Bibliographic Details
Title: Using dynamic biomaterials to study the temporal role of bioactive peptides during osteogenesis.
Authors: Fumasi, Fallon M.1 (AUTHOR), MacCulloch, Tara1,2,3 (AUTHOR), Bernal-Chanchavac, Julio1,2,3 (AUTHOR) jcbernal@asu.edu, Stephanopoulos, Nicholas1,2,3 (AUTHOR) nstepha1@asu.edu, Holloway, Julianne L.1,2 (AUTHOR) Julianne.Holloway@asu.edu
Source: Biomaterials Advances. Feb2024, Vol. 157, pN.PAG-N.PAG. 1p.
Subjects: Bone growth, Peptides, Biomaterials, Hyaluronic acid, Alkaline phosphatase, Cell adhesion molecules
Abstract: The extracellular matrix is a highly dynamic environment, and the precise temporal presentation of biochemical signals is critical for regulating cell behavior during development, healing, and disease progression. To mimic this behavior, we developed a modular DNA-based hydrogel platform to enable independent and reversible control over the immobilization of multiple biomolecules during in vitro cell culture. We combined reversible DNA handles with a norbornene-modified hyaluronic acid hydrogel to orthogonally add and remove multiple biomolecule-DNA conjugates at user-defined timepoints. We demonstrated that the persistent presentation of the cell adhesion peptide RGD was required to maintain cell spreading on hyaluronic acid hydrogels. Further, we discovered the delayed presentation of osteogenic growth peptide (OGP) increased alkaline phosphatase activity compared to other temporal variations. This finding is critically important when considering the design of OGP delivery approaches for bone repair. More broadly, this platform provides a unique approach to tease apart the temporal role of multiple biomolecules during development, regeneration, and disease progression. [Display omitted] • DNA handles enabled orthogonal and reversible control over peptide immobilization. • Persistent immobilization of RGD was required to maintain cell spreading. • The delayed immobilization of osteogenic growth peptide increases osteogenesis. [ABSTRACT FROM AUTHOR]
Copyright of Biomaterials Advances is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 175104629
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Using dynamic biomaterials to study the temporal role of bioactive peptides during osteogenesis.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Fumasi%2C+Fallon+M%2E%22">Fumasi, Fallon M.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22MacCulloch%2C+Tara%22">MacCulloch, Tara</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bernal-Chanchavac%2C+Julio%22">Bernal-Chanchavac, Julio</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<i> jcbernal@asu.edu</i><br /><searchLink fieldCode="AR" term="%22Stephanopoulos%2C+Nicholas%22">Stephanopoulos, Nicholas</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<i> nstepha1@asu.edu</i><br /><searchLink fieldCode="AR" term="%22Holloway%2C+Julianne+L%2E%22">Holloway, Julianne L.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> Julianne.Holloway@asu.edu</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Biomaterials+Advances%22">Biomaterials Advances</searchLink>. Feb2024, Vol. 157, pN.PAG-N.PAG. 1p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Bone+growth%22">Bone growth</searchLink><br /><searchLink fieldCode="DE" term="%22Peptides%22">Peptides</searchLink><br /><searchLink fieldCode="DE" term="%22Biomaterials%22">Biomaterials</searchLink><br /><searchLink fieldCode="DE" term="%22Hyaluronic+acid%22">Hyaluronic acid</searchLink><br /><searchLink fieldCode="DE" term="%22Alkaline+phosphatase%22">Alkaline phosphatase</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+adhesion+molecules%22">Cell adhesion molecules</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The extracellular matrix is a highly dynamic environment, and the precise temporal presentation of biochemical signals is critical for regulating cell behavior during development, healing, and disease progression. To mimic this behavior, we developed a modular DNA-based hydrogel platform to enable independent and reversible control over the immobilization of multiple biomolecules during in vitro cell culture. We combined reversible DNA handles with a norbornene-modified hyaluronic acid hydrogel to orthogonally add and remove multiple biomolecule-DNA conjugates at user-defined timepoints. We demonstrated that the persistent presentation of the cell adhesion peptide RGD was required to maintain cell spreading on hyaluronic acid hydrogels. Further, we discovered the delayed presentation of osteogenic growth peptide (OGP) increased alkaline phosphatase activity compared to other temporal variations. This finding is critically important when considering the design of OGP delivery approaches for bone repair. More broadly, this platform provides a unique approach to tease apart the temporal role of multiple biomolecules during development, regeneration, and disease progression. [Display omitted] • DNA handles enabled orthogonal and reversible control over peptide immobilization. • Persistent immobilization of RGD was required to maintain cell spreading. • The delayed immobilization of osteogenic growth peptide increases osteogenesis. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Biomaterials Advances is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=175104629
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.bioadv.2023.213726
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Bone growth
        Type: general
      – SubjectFull: Peptides
        Type: general
      – SubjectFull: Biomaterials
        Type: general
      – SubjectFull: Hyaluronic acid
        Type: general
      – SubjectFull: Alkaline phosphatase
        Type: general
      – SubjectFull: Cell adhesion molecules
        Type: general
    Titles:
      – TitleFull: Using dynamic biomaterials to study the temporal role of bioactive peptides during osteogenesis.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Fumasi, Fallon M.
      – PersonEntity:
          Name:
            NameFull: MacCulloch, Tara
      – PersonEntity:
          Name:
            NameFull: Bernal-Chanchavac, Julio
      – PersonEntity:
          Name:
            NameFull: Stephanopoulos, Nicholas
      – PersonEntity:
          Name:
            NameFull: Holloway, Julianne L.
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 02
              Text: Feb2024
              Type: published
              Y: 2024
          Identifiers:
            – Type: issn-print
              Value: 27729516
          Numbering:
            – Type: volume
              Value: 157
          Titles:
            – TitleFull: Biomaterials Advances
              Type: main
ResultId 1