Hyperpolarized [1‐13C] pyruvate MRSI to detect metabolic changes in liver in a methionine and choline‐deficient diet rat model of fatty liver disease.
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| Title: | Hyperpolarized [1‐13C] pyruvate MRSI to detect metabolic changes in liver in a methionine and choline‐deficient diet rat model of fatty liver disease. |
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| Authors: | Piraquive Agudelo, Joao1 (AUTHOR), Kim, Yaewon1 (AUTHOR), Agarwal, Shubhangi1 (AUTHOR), Sriram, Renuka1 (AUTHOR), Bok, Robert1 (AUTHOR), Kurhanewicz, John1 (AUTHOR), Mattis, Aras N.2,3 (AUTHOR), Maher, Jacquelyn J.2,4 (AUTHOR), von Morze, Cornelius5 (AUTHOR), Ohliger, Michael A.1,2 (AUTHOR) michael.ohliger@ucsf.edu |
| Source: | Magnetic Resonance in Medicine. Apr2024, Vol. 91 Issue 4, p1625-1636. 12p. |
| Subjects: | Fatty liver, Non-alcoholic fatty liver disease, Methionine, Animal disease models, Diet |
| Abstract: | Purpose: Nonalcoholic fatty liver disease is an important cause of chronic liver disease. There are limited methods for monitoring metabolic changes during progression to steatohepatitis. Hyperpolarized 13C MRSI (HP 13C MRSI) was used to measure metabolic changes in a rodent model of fatty liver disease. Methods: Fifteen Wistar rats were placed on a methionine‐ and choline‐deficient (MCD) diet for 1–18 weeks. HP 13C MRSI, T2‐weighted imaging, and fat‐fraction measurements were obtained at 3 T. Serum aspartate aminotransaminase, alanine aminotransaminase, and triglycerides were measured. Animals were sacrificed for histology and measurement of tissue lactate dehydrogenase (LDH) activity. Results: Animals lost significant weight (13.6% ± 2.34%), an expected characteristic of the MCD diet. Steatosis, inflammation, and mild fibrosis were observed. Liver fat fraction was 31.7% ± 4.5% after 4 weeks and 22.2% ± 4.3% after 9 weeks. Lactate‐to‐pyruvate and alanine‐to‐pyruvate ratios decreased significantly over the study course; were negatively correlated with aspartate aminotransaminase and alanine aminotransaminase (r = −[0.39–0.61]); and were positively correlated with triglycerides (r = 0.59–0.60). Despite observed decreases in hyperpolarized lactate signal, LDH activity increased by a factor of 3 in MCD diet–fed animals. Observed decreases in lactate and alanine hyperpolarized signals on the MCD diet stand in contrast to other studies of liver injury, where lactate and alanine increased. Observed hyperpolarized metabolite changes were not explained by alterations in LDH activity, suggesting that changes may reflect co‐factor depletion known to occur as a result of oxidative stress in the MCD diet. Conclusion: HP 13C MRSI can noninvasively measure metabolic changes in the MCD model of chronic liver disease. [ABSTRACT FROM AUTHOR] |
| Copyright of Magnetic Resonance in Medicine is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Header | DbId: egs DbLabel: Engineering Source An: 175196986 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Hyperpolarized [1‐<superscript>13</superscript>C] pyruvate MRSI to detect metabolic changes in liver in a methionine and choline‐deficient diet rat model of fatty liver disease. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Piraquive+Agudelo%2C+Joao%22">Piraquive Agudelo, Joao</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kim%2C+Yaewon%22">Kim, Yaewon</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Agarwal%2C+Shubhangi%22">Agarwal, Shubhangi</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sriram%2C+Renuka%22">Sriram, Renuka</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bok%2C+Robert%22">Bok, Robert</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kurhanewicz%2C+John%22">Kurhanewicz, John</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mattis%2C+Aras+N%2E%22">Mattis, Aras N.</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Maher%2C+Jacquelyn+J%2E%22">Maher, Jacquelyn J.</searchLink><relatesTo>2,4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22von+Morze%2C+Cornelius%22">von Morze, Cornelius</searchLink><relatesTo>5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ohliger%2C+Michael+A%2E%22">Ohliger, Michael A.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> michael.ohliger@ucsf.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Magnetic+Resonance+in+Medicine%22">Magnetic Resonance in Medicine</searchLink>. Apr2024, Vol. 91 Issue 4, p1625-1636. 12p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Fatty+liver%22">Fatty liver</searchLink><br /><searchLink fieldCode="DE" term="%22Non-alcoholic+fatty+liver+disease%22">Non-alcoholic fatty liver disease</searchLink><br /><searchLink fieldCode="DE" term="%22Methionine%22">Methionine</searchLink><br /><searchLink fieldCode="DE" term="%22Animal+disease+models%22">Animal disease models</searchLink><br /><searchLink fieldCode="DE" term="%22Diet%22">Diet</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Purpose: Nonalcoholic fatty liver disease is an important cause of chronic liver disease. There are limited methods for monitoring metabolic changes during progression to steatohepatitis. Hyperpolarized 13C MRSI (HP 13C MRSI) was used to measure metabolic changes in a rodent model of fatty liver disease. Methods: Fifteen Wistar rats were placed on a methionine‐ and choline‐deficient (MCD) diet for 1–18 weeks. HP 13C MRSI, T2‐weighted imaging, and fat‐fraction measurements were obtained at 3 T. Serum aspartate aminotransaminase, alanine aminotransaminase, and triglycerides were measured. Animals were sacrificed for histology and measurement of tissue lactate dehydrogenase (LDH) activity. Results: Animals lost significant weight (13.6% ± 2.34%), an expected characteristic of the MCD diet. Steatosis, inflammation, and mild fibrosis were observed. Liver fat fraction was 31.7% ± 4.5% after 4 weeks and 22.2% ± 4.3% after 9 weeks. Lactate‐to‐pyruvate and alanine‐to‐pyruvate ratios decreased significantly over the study course; were negatively correlated with aspartate aminotransaminase and alanine aminotransaminase (r = −[0.39–0.61]); and were positively correlated with triglycerides (r = 0.59–0.60). Despite observed decreases in hyperpolarized lactate signal, LDH activity increased by a factor of 3 in MCD diet–fed animals. Observed decreases in lactate and alanine hyperpolarized signals on the MCD diet stand in contrast to other studies of liver injury, where lactate and alanine increased. Observed hyperpolarized metabolite changes were not explained by alterations in LDH activity, suggesting that changes may reflect co‐factor depletion known to occur as a result of oxidative stress in the MCD diet. Conclusion: HP 13C MRSI can noninvasively measure metabolic changes in the MCD model of chronic liver disease. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Magnetic Resonance in Medicine is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/mrm.29954 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 12 StartPage: 1625 Subjects: – SubjectFull: Fatty liver Type: general – SubjectFull: Non-alcoholic fatty liver disease Type: general – SubjectFull: Methionine Type: general – SubjectFull: Animal disease models Type: general – SubjectFull: Diet Type: general Titles: – TitleFull: Hyperpolarized [1‐13C] pyruvate MRSI to detect metabolic changes in liver in a methionine and choline‐deficient diet rat model of fatty liver disease. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Piraquive Agudelo, Joao – PersonEntity: Name: NameFull: Kim, Yaewon – PersonEntity: Name: NameFull: Agarwal, Shubhangi – PersonEntity: Name: NameFull: Sriram, Renuka – PersonEntity: Name: NameFull: Bok, Robert – PersonEntity: Name: NameFull: Kurhanewicz, John – PersonEntity: Name: NameFull: Mattis, Aras N. – PersonEntity: Name: NameFull: Maher, Jacquelyn J. – PersonEntity: Name: NameFull: von Morze, Cornelius – PersonEntity: Name: NameFull: Ohliger, Michael A. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 04 Text: Apr2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 07403194 Numbering: – Type: volume Value: 91 – Type: issue Value: 4 Titles: – TitleFull: Magnetic Resonance in Medicine Type: main |
| ResultId | 1 |
