Three-Dimensional Modeling with Osteoblast-like Cells under External Magnetic Field Conditions Using Magnetic Nano-Ferrite Particles for the Development of Cell-Derived Artificial Bone.
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| Title: | Three-Dimensional Modeling with Osteoblast-like Cells under External Magnetic Field Conditions Using Magnetic Nano-Ferrite Particles for the Development of Cell-Derived Artificial Bone. |
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| Authors: | Ma, Chuang1,2 (AUTHOR) 20hb403b@shinshu-u.ac.jp, Izumiya, Makoto1,2 (AUTHOR) 21hb401k@shinshu-u.ac.jp, Nobuoka, Hidehiko1,3 (AUTHOR) 23bs217f@shinshu-u.ac.jp, Ueno, Rintaro1,3 (AUTHOR) 23bs204d@shinshu-u.ac.jp, Mimura, Masaki1,3 (AUTHOR) 23bs223a@shinshu-u.ac.jp, Ueda, Katsuya1,2 (AUTHOR) 19hb402j@shinshu-u.ac.jp, Ishida, Haruka1,2 (AUTHOR) haruka.i0729@gmail.com, Tomotsune, Daihachiro1,4 (AUTHOR) dtomo@shinshu-u.ac.jp, Johkura, Kohei4 (AUTHOR), Yue, Fengming1,4 (AUTHOR) yueratjp@shinshu-u.ac.jp, Saito, Naoto1 (AUTHOR) saitoko@shinshu-u.ac.jp, Haniu, Hisao1,2,3 (AUTHOR) hhaniu@shinshu-u.ac.jp |
| Source: | Nanomaterials (2079-4991). Feb2024, Vol. 14 Issue 3, p251. 15p. |
| Subjects: | Artificial bones, Magnetic fields, Magnetic particles, Three-dimensional modeling, Bone mechanics, Cell morphology |
| Abstract: | The progress in artificial bone research is crucial for addressing fractures and bone defects in the aging population. However, challenges persist in terms of biocompatibility and structural complexity. Nanotechnology provides a promising avenue by which to overcome these challenges, with nano-ferrite particles (NFPs) exhibiting superparamagnetic properties. The ability to control cell positioning using a magnetic field opens up new possibilities for customizing artificial bones with specific shapes. This study explores the biological effects of NFPs on osteoblast-like cell lines (MC3T3-E1), including key analyses, such as cell viability, cellular uptake of NFPs, calcification processes, cell migration under external magnetic field conditions, and three-dimensional modeling. The results indicate that the impact of NFPs on cell proliferation is negligible. Fluorescence and transmission electron microscopy validated the cellular uptake of NFPs, demonstrating the potential for precise cell positioning through an external magnetic field. Under calcification-inducing conditions, the cells exhibited sustained calcification ability even in the presence of NFPs. The cell movement analysis observed the controlled movement of NFP-absorbing cells under an external magnetic field. Applying a magnetic field along the z-axis induced the three-dimensional shaping of cells incorporating NFPs, resulting in well-arranged z-axis directional patterns. In this study, NFPs demonstrated excellent biocompatibility and controllability under an external magnetic field, laying the foundation for innovative treatment strategies for customizing artificial bones. [ABSTRACT FROM AUTHOR] |
| Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 175369891 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Three-Dimensional Modeling with Osteoblast-like Cells under External Magnetic Field Conditions Using Magnetic Nano-Ferrite Particles for the Development of Cell-Derived Artificial Bone. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Ma%2C+Chuang%22">Ma, Chuang</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> 20hb403b@shinshu-u.ac.jp</i><br /><searchLink fieldCode="AR" term="%22Izumiya%2C+Makoto%22">Izumiya, Makoto</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> 21hb401k@shinshu-u.ac.jp</i><br /><searchLink fieldCode="AR" term="%22Nobuoka%2C+Hidehiko%22">Nobuoka, Hidehiko</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)<i> 23bs217f@shinshu-u.ac.jp</i><br /><searchLink fieldCode="AR" term="%22Ueno%2C+Rintaro%22">Ueno, Rintaro</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)<i> 23bs204d@shinshu-u.ac.jp</i><br /><searchLink fieldCode="AR" term="%22Mimura%2C+Masaki%22">Mimura, Masaki</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)<i> 23bs223a@shinshu-u.ac.jp</i><br /><searchLink fieldCode="AR" term="%22Ueda%2C+Katsuya%22">Ueda, Katsuya</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> 19hb402j@shinshu-u.ac.jp</i><br /><searchLink fieldCode="AR" term="%22Ishida%2C+Haruka%22">Ishida, Haruka</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> haruka.i0729@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Tomotsune%2C+Daihachiro%22">Tomotsune, Daihachiro</searchLink><relatesTo>1,4</relatesTo> (AUTHOR)<i> dtomo@shinshu-u.ac.jp</i><br /><searchLink fieldCode="AR" term="%22Johkura%2C+Kohei%22">Johkura, Kohei</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yue%2C+Fengming%22">Yue, Fengming</searchLink><relatesTo>1,4</relatesTo> (AUTHOR)<i> yueratjp@shinshu-u.ac.jp</i><br /><searchLink fieldCode="AR" term="%22Saito%2C+Naoto%22">Saito, Naoto</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> saitoko@shinshu-u.ac.jp</i><br /><searchLink fieldCode="AR" term="%22Haniu%2C+Hisao%22">Haniu, Hisao</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<i> hhaniu@shinshu-u.ac.jp</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Nanomaterials+%282079-4991%29%22">Nanomaterials (2079-4991)</searchLink>. Feb2024, Vol. 14 Issue 3, p251. 15p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Artificial+bones%22">Artificial bones</searchLink><br /><searchLink fieldCode="DE" term="%22Magnetic+fields%22">Magnetic fields</searchLink><br /><searchLink fieldCode="DE" term="%22Magnetic+particles%22">Magnetic particles</searchLink><br /><searchLink fieldCode="DE" term="%22Three-dimensional+modeling%22">Three-dimensional modeling</searchLink><br /><searchLink fieldCode="DE" term="%22Bone+mechanics%22">Bone mechanics</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+morphology%22">Cell morphology</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The progress in artificial bone research is crucial for addressing fractures and bone defects in the aging population. However, challenges persist in terms of biocompatibility and structural complexity. Nanotechnology provides a promising avenue by which to overcome these challenges, with nano-ferrite particles (NFPs) exhibiting superparamagnetic properties. The ability to control cell positioning using a magnetic field opens up new possibilities for customizing artificial bones with specific shapes. This study explores the biological effects of NFPs on osteoblast-like cell lines (MC3T3-E1), including key analyses, such as cell viability, cellular uptake of NFPs, calcification processes, cell migration under external magnetic field conditions, and three-dimensional modeling. The results indicate that the impact of NFPs on cell proliferation is negligible. Fluorescence and transmission electron microscopy validated the cellular uptake of NFPs, demonstrating the potential for precise cell positioning through an external magnetic field. Under calcification-inducing conditions, the cells exhibited sustained calcification ability even in the presence of NFPs. The cell movement analysis observed the controlled movement of NFP-absorbing cells under an external magnetic field. Applying a magnetic field along the z-axis induced the three-dimensional shaping of cells incorporating NFPs, resulting in well-arranged z-axis directional patterns. In this study, NFPs demonstrated excellent biocompatibility and controllability under an external magnetic field, laying the foundation for innovative treatment strategies for customizing artificial bones. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3390/nano14030251 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 15 StartPage: 251 Subjects: – SubjectFull: Artificial bones Type: general – SubjectFull: Magnetic fields Type: general – SubjectFull: Magnetic particles Type: general – SubjectFull: Three-dimensional modeling Type: general – SubjectFull: Bone mechanics Type: general – SubjectFull: Cell morphology Type: general Titles: – TitleFull: Three-Dimensional Modeling with Osteoblast-like Cells under External Magnetic Field Conditions Using Magnetic Nano-Ferrite Particles for the Development of Cell-Derived Artificial Bone. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Ma, Chuang – PersonEntity: Name: NameFull: Izumiya, Makoto – PersonEntity: Name: NameFull: Nobuoka, Hidehiko – PersonEntity: Name: NameFull: Ueno, Rintaro – PersonEntity: Name: NameFull: Mimura, Masaki – PersonEntity: Name: NameFull: Ueda, Katsuya – PersonEntity: Name: NameFull: Ishida, Haruka – PersonEntity: Name: NameFull: Tomotsune, Daihachiro – PersonEntity: Name: NameFull: Johkura, Kohei – PersonEntity: Name: NameFull: Yue, Fengming – PersonEntity: Name: NameFull: Saito, Naoto – PersonEntity: Name: NameFull: Haniu, Hisao IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 02 Text: Feb2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 20794991 Numbering: – Type: volume Value: 14 – Type: issue Value: 3 Titles: – TitleFull: Nanomaterials (2079-4991) Type: main |
| ResultId | 1 |
