Design of a dual-responding genetic circuit for high-throughput identification of L-threonine-overproducing Escherichia coli.
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| Title: | Design of a dual-responding genetic circuit for high-throughput identification of L-threonine-overproducing Escherichia coli. |
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| Authors: | Su, Buli1 (AUTHOR) bolysu@hotmail.com, Lai, Peixuan1 (AUTHOR) 522019158@qq.com, Deng, Ming-Rong1 (AUTHOR) dengmr@gdim.cn, Zhu, Honghui1 (AUTHOR) zhuhh_gdim@163.com |
| Source: | Bioresource Technology. Mar2024, Vol. 395, pN.PAG-N.PAG. 1p. |
| Subjects: | Threonine, Escherichia coli, Metabolic regulation, High throughput screening (Drug development), Animal feeds, Biosensors |
| Abstract: | [Display omitted] • The inducer-like effect of L-threonine was firstly demonstrated. • A dual-responding genetic circuit was developed as L-threonine biosensor. • The developed HTS platform could identify mutants from a large-scale RBS library. • The HTS platform was applied in direction evolution of the key enzyme thrA. • L-threonine production increased 7-fold through directed evolution of the key enzyme. L-threonine is a crucial amino acid that is extensively employed in the realms of food, animal feed and pharmaceuticals. Unfortunately, the lack of an appropriate biosensor has hindered the establishment of a robust high-throughput screening (HTS) system for the identification of the desired strains from random mutants. In this study, a dual-responding genetic circuit that capitalizes on the L-threonine inducer-like effect, the L-threonine riboswitch, and a signal amplification system was designed for the purpose of screening L-threonine overproducers. This platform effectively enhanced the performance of the enzyme and facilitated the identification of high L-threonine-producing strains from a random mutant library. Consequently, pathway optimization and directed evolution of the key enzyme enhanced L-threonine production by 4 and 7-fold, respectively. These results demonstrate the potential of biosensor design for dynamic metabolite detection and offer a promising tool for HTS and metabolic regulation for the development of L-threonine-hyperproducing strains. [ABSTRACT FROM AUTHOR] |
| Copyright of Bioresource Technology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 175454466 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Design of a dual-responding genetic circuit for high-throughput identification of L-threonine-overproducing Escherichia coli. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Su%2C+Buli%22">Su, Buli</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> bolysu@hotmail.com</i><br /><searchLink fieldCode="AR" term="%22Lai%2C+Peixuan%22">Lai, Peixuan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> 522019158@qq.com</i><br /><searchLink fieldCode="AR" term="%22Deng%2C+Ming-Rong%22">Deng, Ming-Rong</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> dengmr@gdim.cn</i><br /><searchLink fieldCode="AR" term="%22Zhu%2C+Honghui%22">Zhu, Honghui</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> zhuhh_gdim@163.com</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Bioresource+Technology%22">Bioresource Technology</searchLink>. Mar2024, Vol. 395, pN.PAG-N.PAG. 1p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Threonine%22">Threonine</searchLink><br /><searchLink fieldCode="DE" term="%22Escherichia+coli%22">Escherichia coli</searchLink><br /><searchLink fieldCode="DE" term="%22Metabolic+regulation%22">Metabolic regulation</searchLink><br /><searchLink fieldCode="DE" term="%22High+throughput+screening+%28Drug+development%29%22">High throughput screening (Drug development)</searchLink><br /><searchLink fieldCode="DE" term="%22Animal+feeds%22">Animal feeds</searchLink><br /><searchLink fieldCode="DE" term="%22Biosensors%22">Biosensors</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: [Display omitted] • The inducer-like effect of L-threonine was firstly demonstrated. • A dual-responding genetic circuit was developed as L-threonine biosensor. • The developed HTS platform could identify mutants from a large-scale RBS library. • The HTS platform was applied in direction evolution of the key enzyme thrA. • L-threonine production increased 7-fold through directed evolution of the key enzyme. L-threonine is a crucial amino acid that is extensively employed in the realms of food, animal feed and pharmaceuticals. Unfortunately, the lack of an appropriate biosensor has hindered the establishment of a robust high-throughput screening (HTS) system for the identification of the desired strains from random mutants. In this study, a dual-responding genetic circuit that capitalizes on the L-threonine inducer-like effect, the L-threonine riboswitch, and a signal amplification system was designed for the purpose of screening L-threonine overproducers. This platform effectively enhanced the performance of the enzyme and facilitated the identification of high L-threonine-producing strains from a random mutant library. Consequently, pathway optimization and directed evolution of the key enzyme enhanced L-threonine production by 4 and 7-fold, respectively. These results demonstrate the potential of biosensor design for dynamic metabolite detection and offer a promising tool for HTS and metabolic regulation for the development of L-threonine-hyperproducing strains. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Bioresource Technology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.biortech.2024.130407 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 1 StartPage: N.PAG Subjects: – SubjectFull: Threonine Type: general – SubjectFull: Escherichia coli Type: general – SubjectFull: Metabolic regulation Type: general – SubjectFull: High throughput screening (Drug development) Type: general – SubjectFull: Animal feeds Type: general – SubjectFull: Biosensors Type: general Titles: – TitleFull: Design of a dual-responding genetic circuit for high-throughput identification of L-threonine-overproducing Escherichia coli. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Su, Buli – PersonEntity: Name: NameFull: Lai, Peixuan – PersonEntity: Name: NameFull: Deng, Ming-Rong – PersonEntity: Name: NameFull: Zhu, Honghui IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: Mar2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 09608524 Numbering: – Type: volume Value: 395 Titles: – TitleFull: Bioresource Technology Type: main |
| ResultId | 1 |
