View in EDS HTML Full Text PDF Full Text

In vitro photoinactivation effectiveness of a portable LED device aimed for intranasal photodisinfection and a photosensitizer formulation comprising methylene blue and potassium iodide against bacterial, fungal, and viral respiratory pathogens.

Saved in:
Bibliographic Details
Title: In vitro photoinactivation effectiveness of a portable LED device aimed for intranasal photodisinfection and a photosensitizer formulation comprising methylene blue and potassium iodide against bacterial, fungal, and viral respiratory pathogens.
Authors: Chakraborty, Sourabrata1 (AUTHOR), Mohanty, Deepanwita1 (AUTHOR), Chowdhury, Anupam1 (AUTHOR), Krishna, Hemant1 (AUTHOR), Taraphdar, Debjani2 (AUTHOR), Chitnis, Sheetal2 (AUTHOR), Sodani, Sadhna2 (AUTHOR), Sahu, Khageswar1,3 (AUTHOR) khageswar@rrcat.gov.in, Majumder, Shovan Kumar1,3 (AUTHOR)
Source: Lasers in Medical Science. 2/14/2024, Vol. 39 Issue 1, p1-11. 11p.
Subjects: Colony-forming units assay, Methylene blue, Potassium iodide, Red light, Methicillin-resistant staphylococcus aureus, Pathogenic microorganisms, Parainfluenza viruses, Klebsiella pneumoniae
Abstract: Antimicrobial photodynamic therapy (aPDT) can be a viable option for management of intranasal infections. However, there are light delivery, fluence, and photosensitizer-related challenges. We report in vitro effectiveness of an easily fabricated, low-cost, portable, LED device and a formulation comprising methylene blue (MB) and potassium iodide (KI) for photoinactivation of pathogens of the nasal cavity, namely, methicillin-resistant Staphylococcus aureus, antibiotic-resistant Klebsiella pneumoniae, multi-antibiotic-resistant Pseudomonas aeruginosa, Candida spp., and SARS-CoV-2. In a 96-well plate, microbial suspensions incubated with 0.005% MB alone or MB and KI formulation were exposed to different red light (~ 660 ± 25 nm) fluence using the LED device fitted to each well. Survival loss in bacteria and fungi was quantified using colony-forming unit assay, and SARS-CoV-2 photodamage was assessed by RT-PCR. The results suggest that KI addition to MB leads to KI concentration-dependent potentiation (up to ~ 5 log10) of photoinactivation in bacteria and fungi. aPDT in the presence of 25 or 50 mM KI shows the following photoinactivation trend; Gm + ve bacteria > Gm − ve bacteria > fungi > virus. aPDT in the presence of 100 mM KI, using 3- or 5-min red light exposure, results in complete eradication of bacteria or fungi, respectively. For SARS-CoV-2, aPDT using MB-KI leads to a ~ 6.5 increase in cycle threshold value. The results demonstrate the photoinactivation effectiveness of the device and MB-KI formulation, which may be helpful in designing of an optimized protocol for future intranasal photoinactivation studies in clinical settings. [ABSTRACT FROM AUTHOR]
Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Full text is not displayed to guests.
FullText Links:
  – Type: pdflink
Text:
  Availability: 1
Header DbId: egs
DbLabel: Engineering Source
An: 175459363
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: In vitro photoinactivation effectiveness of a portable LED device aimed for intranasal photodisinfection and a photosensitizer formulation comprising methylene blue and potassium iodide against bacterial, fungal, and viral respiratory pathogens.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Chakraborty%2C+Sourabrata%22">Chakraborty, Sourabrata</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mohanty%2C+Deepanwita%22">Mohanty, Deepanwita</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chowdhury%2C+Anupam%22">Chowdhury, Anupam</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Krishna%2C+Hemant%22">Krishna, Hemant</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Taraphdar%2C+Debjani%22">Taraphdar, Debjani</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chitnis%2C+Sheetal%22">Chitnis, Sheetal</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sodani%2C+Sadhna%22">Sodani, Sadhna</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sahu%2C+Khageswar%22">Sahu, Khageswar</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)<i> khageswar@rrcat.gov.in</i><br /><searchLink fieldCode="AR" term="%22Majumder%2C+Shovan+Kumar%22">Majumder, Shovan Kumar</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Lasers+in+Medical+Science%22">Lasers in Medical Science</searchLink>. 2/14/2024, Vol. 39 Issue 1, p1-11. 11p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Colony-forming+units+assay%22">Colony-forming units assay</searchLink><br /><searchLink fieldCode="DE" term="%22Methylene+blue%22">Methylene blue</searchLink><br /><searchLink fieldCode="DE" term="%22Potassium+iodide%22">Potassium iodide</searchLink><br /><searchLink fieldCode="DE" term="%22Red+light%22">Red light</searchLink><br /><searchLink fieldCode="DE" term="%22Methicillin-resistant+staphylococcus+aureus%22">Methicillin-resistant staphylococcus aureus</searchLink><br /><searchLink fieldCode="DE" term="%22Pathogenic+microorganisms%22">Pathogenic microorganisms</searchLink><br /><searchLink fieldCode="DE" term="%22Parainfluenza+viruses%22">Parainfluenza viruses</searchLink><br /><searchLink fieldCode="DE" term="%22Klebsiella+pneumoniae%22">Klebsiella pneumoniae</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Antimicrobial photodynamic therapy (aPDT) can be a viable option for management of intranasal infections. However, there are light delivery, fluence, and photosensitizer-related challenges. We report in vitro effectiveness of an easily fabricated, low-cost, portable, LED device and a formulation comprising methylene blue (MB) and potassium iodide (KI) for photoinactivation of pathogens of the nasal cavity, namely, methicillin-resistant Staphylococcus aureus, antibiotic-resistant Klebsiella pneumoniae, multi-antibiotic-resistant Pseudomonas aeruginosa, Candida spp., and SARS-CoV-2. In a 96-well plate, microbial suspensions incubated with 0.005% MB alone or MB and KI formulation were exposed to different red light (~ 660 ± 25 nm) fluence using the LED device fitted to each well. Survival loss in bacteria and fungi was quantified using colony-forming unit assay, and SARS-CoV-2 photodamage was assessed by RT-PCR. The results suggest that KI addition to MB leads to KI concentration-dependent potentiation (up to ~ 5 log10) of photoinactivation in bacteria and fungi. aPDT in the presence of 25 or 50 mM KI shows the following photoinactivation trend; Gm + ve bacteria > Gm − ve bacteria > fungi > virus. aPDT in the presence of 100 mM KI, using 3- or 5-min red light exposure, results in complete eradication of bacteria or fungi, respectively. For SARS-CoV-2, aPDT using MB-KI leads to a ~ 6.5 increase in cycle threshold value. The results demonstrate the photoinactivation effectiveness of the device and MB-KI formulation, which may be helpful in designing of an optimized protocol for future intranasal photoinactivation studies in clinical settings. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=175459363
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1007/s10103-024-03996-2
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 11
        StartPage: 1
    Subjects:
      – SubjectFull: Colony-forming units assay
        Type: general
      – SubjectFull: Methylene blue
        Type: general
      – SubjectFull: Potassium iodide
        Type: general
      – SubjectFull: Red light
        Type: general
      – SubjectFull: Methicillin-resistant staphylococcus aureus
        Type: general
      – SubjectFull: Pathogenic microorganisms
        Type: general
      – SubjectFull: Parainfluenza viruses
        Type: general
      – SubjectFull: Klebsiella pneumoniae
        Type: general
    Titles:
      – TitleFull: In vitro photoinactivation effectiveness of a portable LED device aimed for intranasal photodisinfection and a photosensitizer formulation comprising methylene blue and potassium iodide against bacterial, fungal, and viral respiratory pathogens.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Chakraborty, Sourabrata
      – PersonEntity:
          Name:
            NameFull: Mohanty, Deepanwita
      – PersonEntity:
          Name:
            NameFull: Chowdhury, Anupam
      – PersonEntity:
          Name:
            NameFull: Krishna, Hemant
      – PersonEntity:
          Name:
            NameFull: Taraphdar, Debjani
      – PersonEntity:
          Name:
            NameFull: Chitnis, Sheetal
      – PersonEntity:
          Name:
            NameFull: Sodani, Sadhna
      – PersonEntity:
          Name:
            NameFull: Sahu, Khageswar
      – PersonEntity:
          Name:
            NameFull: Majumder, Shovan Kumar
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 14
              M: 02
              Text: 2/14/2024
              Type: published
              Y: 2024
          Identifiers:
            – Type: issn-print
              Value: 02688921
          Numbering:
            – Type: volume
              Value: 39
            – Type: issue
              Value: 1
          Titles:
            – TitleFull: Lasers in Medical Science
              Type: main
ResultId 1