Central composite design‐based response surface methodology for optimization of tetrodotoxin extraction from the horseshoe crab Carcinoscorpius rotundicauda.
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| Title: | Central composite design‐based response surface methodology for optimization of tetrodotoxin extraction from the horseshoe crab Carcinoscorpius rotundicauda. |
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| Authors: | Tran, Dang Thuan1 (AUTHOR) tdangthuan@ich.vast.vn, Dinh, Cuc T.1 (AUTHOR), Dang, Mai T.1 (AUTHOR), Do, Cam Van T.2 (AUTHOR), Le Ho, Khanh Hy3 (AUTHOR), Le, Truong Giang1,4 (AUTHOR), Dao, Viet Ha3,4 (AUTHOR) |
| Source: | Asia-Pacific Journal of Chemical Engineering. Jan/Feb2024, Vol. 19 Issue 1, p1-13. 13p. |
| Subjects: | Response surfaces (Statistics), Limulidae, Tetrodotoxin, Aquatic animals, Acetic acid |
| Geographic Terms: | Vietnam |
| Abstract: | Tetrodotoxin analogues (TTXs) used in research and development were originated from biological sources for the last decades. Among TTXs‐bearing aquatic animals, horseshoe crab Carcinoscorpius rotundicauda from Vietnam's Coast was reported to contain a high level of TTXs, indicating a promising source for extraction and purification of TTXs. In this work, horseshoe crab C. rotundicauda collected from Can Gio Coast, Vietnam was used for TTX extraction using homogenization‐assisted method with acetic acid solvent. The extraction process was analysed and optimized by single‐factor investigation and response surface methodology (RSM). Single‐factor investigation pointed out that the optimal conditions for TTX recovery yield from C. rotundicauda's soft tissues were homogenization time of 20 min, acetic acid concentration of 1.0%, solvent/matrix ratio of 4 mL/g, extraction temperature of 70°C, extraction time of 30 min and number of extraction of two times. The most significant factors including acetic acid concentration, solvent/matrix ratio and extraction temperature were utilized for constructing the RSM model to predict TTX recovery yield. The maximal TTX recovery yield predicted from RSM model was 97.3% under solvent/matrix ratio of 4 mL/g, extraction temperature of 70°C and acetic acid concentration of 1% (v/v). The RSM model displayed a high stability and precision for prediction of TTX recovery yield from experiments of 1–10 kg C. rotundicauda's soft tissues/batch extractions producing low errors (−1.95% to 1.85%). TTXs in C. rotundicauda's soft tissues were elucidated by HPLC‐MS/MS, revealing the presence of three analogues TTX, 4epi‐TTX and Anh‐TTX with their contents of 43.02, 22.54 and 45.07 μg/g, respectively. [ABSTRACT FROM AUTHOR] |
| Copyright of Asia-Pacific Journal of Chemical Engineering is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Header | DbId: egs DbLabel: Engineering Source An: 175502744 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Central composite design‐based response surface methodology for optimization of tetrodotoxin extraction from the horseshoe crab Carcinoscorpius rotundicauda. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Tran%2C+Dang+Thuan%22">Tran, Dang Thuan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> tdangthuan@ich.vast.vn</i><br /><searchLink fieldCode="AR" term="%22Dinh%2C+Cuc+T%2E%22">Dinh, Cuc T.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Dang%2C+Mai+T%2E%22">Dang, Mai T.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Do%2C+Cam+Van+T%2E%22">Do, Cam Van T.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Le+Ho%2C+Khanh+Hy%22">Le Ho, Khanh Hy</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Le%2C+Truong+Giang%22">Le, Truong Giang</searchLink><relatesTo>1,4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Dao%2C+Viet+Ha%22">Dao, Viet Ha</searchLink><relatesTo>3,4</relatesTo> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Asia-Pacific+Journal+of+Chemical+Engineering%22">Asia-Pacific Journal of Chemical Engineering</searchLink>. Jan/Feb2024, Vol. 19 Issue 1, p1-13. 13p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Response+surfaces+%28Statistics%29%22">Response surfaces (Statistics)</searchLink><br /><searchLink fieldCode="DE" term="%22Limulidae%22">Limulidae</searchLink><br /><searchLink fieldCode="DE" term="%22Tetrodotoxin%22">Tetrodotoxin</searchLink><br /><searchLink fieldCode="DE" term="%22Aquatic+animals%22">Aquatic animals</searchLink><br /><searchLink fieldCode="DE" term="%22Acetic+acid%22">Acetic acid</searchLink> – Name: SubjectGeographic Label: Geographic Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Vietnam%22">Vietnam</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Tetrodotoxin analogues (TTXs) used in research and development were originated from biological sources for the last decades. Among TTXs‐bearing aquatic animals, horseshoe crab Carcinoscorpius rotundicauda from Vietnam's Coast was reported to contain a high level of TTXs, indicating a promising source for extraction and purification of TTXs. In this work, horseshoe crab C. rotundicauda collected from Can Gio Coast, Vietnam was used for TTX extraction using homogenization‐assisted method with acetic acid solvent. The extraction process was analysed and optimized by single‐factor investigation and response surface methodology (RSM). Single‐factor investigation pointed out that the optimal conditions for TTX recovery yield from C. rotundicauda's soft tissues were homogenization time of 20 min, acetic acid concentration of 1.0%, solvent/matrix ratio of 4 mL/g, extraction temperature of 70°C, extraction time of 30 min and number of extraction of two times. The most significant factors including acetic acid concentration, solvent/matrix ratio and extraction temperature were utilized for constructing the RSM model to predict TTX recovery yield. The maximal TTX recovery yield predicted from RSM model was 97.3% under solvent/matrix ratio of 4 mL/g, extraction temperature of 70°C and acetic acid concentration of 1% (v/v). The RSM model displayed a high stability and precision for prediction of TTX recovery yield from experiments of 1–10 kg C. rotundicauda's soft tissues/batch extractions producing low errors (−1.95% to 1.85%). TTXs in C. rotundicauda's soft tissues were elucidated by HPLC‐MS/MS, revealing the presence of three analogues TTX, 4epi‐TTX and Anh‐TTX with their contents of 43.02, 22.54 and 45.07 μg/g, respectively. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Asia-Pacific Journal of Chemical Engineering is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/apj.2974 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 13 StartPage: 1 Subjects: – SubjectFull: Response surfaces (Statistics) Type: general – SubjectFull: Limulidae Type: general – SubjectFull: Tetrodotoxin Type: general – SubjectFull: Aquatic animals Type: general – SubjectFull: Acetic acid Type: general – SubjectFull: Vietnam Type: general Titles: – TitleFull: Central composite design‐based response surface methodology for optimization of tetrodotoxin extraction from the horseshoe crab Carcinoscorpius rotundicauda. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Tran, Dang Thuan – PersonEntity: Name: NameFull: Dinh, Cuc T. – PersonEntity: Name: NameFull: Dang, Mai T. – PersonEntity: Name: NameFull: Do, Cam Van T. – PersonEntity: Name: NameFull: Le Ho, Khanh Hy – PersonEntity: Name: NameFull: Le, Truong Giang – PersonEntity: Name: NameFull: Dao, Viet Ha IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: Jan/Feb2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 19322135 Numbering: – Type: volume Value: 19 – Type: issue Value: 1 Titles: – TitleFull: Asia-Pacific Journal of Chemical Engineering Type: main |
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