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The Effect of Substrate Properties on Cellular Behavior and Nanoparticle Uptake in Human Fibroblasts and Epithelial Cells.

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Title: The Effect of Substrate Properties on Cellular Behavior and Nanoparticle Uptake in Human Fibroblasts and Epithelial Cells.
Authors: Sousa de Almeida, Mauro1 (AUTHOR) mauro.sousadealmeida@unifr.ch, Lee, Aaron1,2 (AUTHOR) a.lee22@imperial.ac.uk, Itel, Fabian3 (AUTHOR) fabian.itel@empa.ch, Maniura-Weber, Katharina4 (AUTHOR) katharina.maniura@empa.ch, Petri-Fink, Alke1,5 (AUTHOR) alke.fink@unifr.ch, Rothen-Rutishauser, Barbara1 (AUTHOR) barbara.rothen@unifr.ch
Source: Nanomaterials (2079-4991). Feb2024, Vol. 14 Issue 4, p342. 16p.
Subjects: Epithelial cells, Nanoparticles, Fibroblasts, Epithelial cell culture, Tissue mechanics, Polyurethane elastomers
Abstract: The delivery of nanomedicines into cells holds enormous therapeutic potential; however little is known regarding how the extracellular matrix (ECM) can influence cell–nanoparticle (NP) interactions. Changes in ECM organization and composition occur in several pathophysiological states, including fibrosis and tumorigenesis, and may contribute to disease progression. We show that the physical characteristics of cellular substrates, that more closely resemble the ECM in vivo, can influence cell behavior and the subsequent uptake of NPs. Electrospinning was used to create two different substrates made of soft polyurethane (PU) with aligned and non-aligned nanofibers to recapitulate the ECM in two different states. To investigate the impact of cell–substrate interaction, A549 lung epithelial cells and MRC-5 lung fibroblasts were cultured on soft PU membranes with different alignments and compared against stiff tissue culture plastic (TCP)/glass. Both cell types could attach and grow on both PU membranes with no signs of cytotoxicity but with increased cytokine release compared with cells on the TCP. The uptake of silica NPs increased more than three-fold in fibroblasts but not in epithelial cells cultured on both membranes. This study demonstrates that cell–matrix interaction is substrate and cell-type dependent and highlights the importance of considering the ECM and tissue mechanical properties when designing NPs for effective cell targeting and treatment. [ABSTRACT FROM AUTHOR]
Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: The Effect of Substrate Properties on Cellular Behavior and Nanoparticle Uptake in Human Fibroblasts and Epithelial Cells.
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  Data: <searchLink fieldCode="AR" term="%22Sousa+de+Almeida%2C+Mauro%22">Sousa de Almeida, Mauro</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> mauro.sousadealmeida@unifr.ch</i><br /><searchLink fieldCode="AR" term="%22Lee%2C+Aaron%22">Lee, Aaron</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> a.lee22@imperial.ac.uk</i><br /><searchLink fieldCode="AR" term="%22Itel%2C+Fabian%22">Itel, Fabian</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> fabian.itel@empa.ch</i><br /><searchLink fieldCode="AR" term="%22Maniura-Weber%2C+Katharina%22">Maniura-Weber, Katharina</searchLink><relatesTo>4</relatesTo> (AUTHOR)<i> katharina.maniura@empa.ch</i><br /><searchLink fieldCode="AR" term="%22Petri-Fink%2C+Alke%22">Petri-Fink, Alke</searchLink><relatesTo>1,5</relatesTo> (AUTHOR)<i> alke.fink@unifr.ch</i><br /><searchLink fieldCode="AR" term="%22Rothen-Rutishauser%2C+Barbara%22">Rothen-Rutishauser, Barbara</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> barbara.rothen@unifr.ch</i>
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  Data: <searchLink fieldCode="JN" term="%22Nanomaterials+%282079-4991%29%22">Nanomaterials (2079-4991)</searchLink>. Feb2024, Vol. 14 Issue 4, p342. 16p.
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  Data: <searchLink fieldCode="DE" term="%22Epithelial+cells%22">Epithelial cells</searchLink><br /><searchLink fieldCode="DE" term="%22Nanoparticles%22">Nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Fibroblasts%22">Fibroblasts</searchLink><br /><searchLink fieldCode="DE" term="%22Epithelial+cell+culture%22">Epithelial cell culture</searchLink><br /><searchLink fieldCode="DE" term="%22Tissue+mechanics%22">Tissue mechanics</searchLink><br /><searchLink fieldCode="DE" term="%22Polyurethane+elastomers%22">Polyurethane elastomers</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: The delivery of nanomedicines into cells holds enormous therapeutic potential; however little is known regarding how the extracellular matrix (ECM) can influence cell–nanoparticle (NP) interactions. Changes in ECM organization and composition occur in several pathophysiological states, including fibrosis and tumorigenesis, and may contribute to disease progression. We show that the physical characteristics of cellular substrates, that more closely resemble the ECM in vivo, can influence cell behavior and the subsequent uptake of NPs. Electrospinning was used to create two different substrates made of soft polyurethane (PU) with aligned and non-aligned nanofibers to recapitulate the ECM in two different states. To investigate the impact of cell–substrate interaction, A549 lung epithelial cells and MRC-5 lung fibroblasts were cultured on soft PU membranes with different alignments and compared against stiff tissue culture plastic (TCP)/glass. Both cell types could attach and grow on both PU membranes with no signs of cytotoxicity but with increased cytokine release compared with cells on the TCP. The uptake of silica NPs increased more than three-fold in fibroblasts but not in epithelial cells cultured on both membranes. This study demonstrates that cell–matrix interaction is substrate and cell-type dependent and highlights the importance of considering the ECM and tissue mechanical properties when designing NPs for effective cell targeting and treatment. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.3390/nano14040342
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      – Code: eng
        Text: English
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        PageCount: 16
        StartPage: 342
    Subjects:
      – SubjectFull: Epithelial cells
        Type: general
      – SubjectFull: Nanoparticles
        Type: general
      – SubjectFull: Fibroblasts
        Type: general
      – SubjectFull: Epithelial cell culture
        Type: general
      – SubjectFull: Tissue mechanics
        Type: general
      – SubjectFull: Polyurethane elastomers
        Type: general
    Titles:
      – TitleFull: The Effect of Substrate Properties on Cellular Behavior and Nanoparticle Uptake in Human Fibroblasts and Epithelial Cells.
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            NameFull: Sousa de Almeida, Mauro
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            NameFull: Lee, Aaron
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            NameFull: Itel, Fabian
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              M: 02
              Text: Feb2024
              Type: published
              Y: 2024
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              Value: 14
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