Sub-Nanomolar Detection of Oligonucleotides Using Molecular Beacons Immobilized on Lightguiding Nanowires.
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| Title: | Sub-Nanomolar Detection of Oligonucleotides Using Molecular Beacons Immobilized on Lightguiding Nanowires. |
|---|---|
| Authors: | Johansson, Therese B.1,2 (AUTHOR) therese.johansson@ftf.lth.se, Davtyan, Rubina1,2 (AUTHOR) julia.valderas_gutierrez@ftf.lth.se, Valderas-Gutiérrez, Julia1,2 (AUTHOR), Gonzalez Rodriguez, Adrian3 (AUTHOR), Agnarsson, Björn3 (AUTHOR), Munita, Roberto4 (AUTHOR), Fioretos, Thoas5 (AUTHOR), Lilljebjörn, Henrik5 (AUTHOR), Linke, Heiner1,2 (AUTHOR), Höök, Fredrik2,3 (AUTHOR) fredrik.hook@chalmers.se, Prinz, Christelle N.1,2 (AUTHOR) fredrik.hook@chalmers.se |
| Source: | Nanomaterials (2079-4991). Mar2024, Vol. 14 Issue 5, p453. 11p. |
| Subjects: | Oligonucleotides, Nanowires, Light cones, Signal-to-noise ratio, Detection limit, Emission standards |
| Abstract: | The detection of oligonucleotides is a central step in many biomedical investigations. The most commonly used methods for detecting oligonucleotides often require concentration and amplification before detection. Therefore, developing detection methods with a direct read-out would be beneficial. Although commonly used for the detection of amplified oligonucleotides, fluorescent molecular beacons have been proposed for such direct detection. However, the reported limits of detection using molecular beacons are relatively high, ranging from 100 nM to a few µM, primarily limited by the beacon fluorescence background. In this study, we enhanced the relative signal contrast between hybridized and non-hybridized states of the beacons by immobilizing them on lightguiding nanowires. Upon hybridization to a complementary oligonucleotide, the fluorescence from the surface-bound beacon becomes coupled in the lightguiding nanowire core and is re-emitted at the nanowire tip in a narrower cone of light compared with the standard 4π emission. Prior knowledge of the nanowire positions allows for the continuous monitoring of fluorescence signals from each nanowire, which effectively facilitates the discrimination of signals arising from hybridization events against background signals. This resulted in improved signal-to-background and signal-to-noise ratios, which allowed for the direct detection of oligonucleotides at a concentration as low as 0.1 nM. [ABSTRACT FROM AUTHOR] |
| Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 175991968 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Sub-Nanomolar Detection of Oligonucleotides Using Molecular Beacons Immobilized on Lightguiding Nanowires. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Johansson%2C+Therese+B%2E%22">Johansson, Therese B.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> therese.johansson@ftf.lth.se</i><br /><searchLink fieldCode="AR" term="%22Davtyan%2C+Rubina%22">Davtyan, Rubina</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> julia.valderas_gutierrez@ftf.lth.se</i><br /><searchLink fieldCode="AR" term="%22Valderas-Gutiérrez%2C+Julia%22">Valderas-Gutiérrez, Julia</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Gonzalez+Rodriguez%2C+Adrian%22">Gonzalez Rodriguez, Adrian</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Agnarsson%2C+Björn%22">Agnarsson, Björn</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Munita%2C+Roberto%22">Munita, Roberto</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Fioretos%2C+Thoas%22">Fioretos, Thoas</searchLink><relatesTo>5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lilljebjörn%2C+Henrik%22">Lilljebjörn, Henrik</searchLink><relatesTo>5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Linke%2C+Heiner%22">Linke, Heiner</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Höök%2C+Fredrik%22">Höök, Fredrik</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)<i> fredrik.hook@chalmers.se</i><br /><searchLink fieldCode="AR" term="%22Prinz%2C+Christelle+N%2E%22">Prinz, Christelle N.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> fredrik.hook@chalmers.se</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Nanomaterials+%282079-4991%29%22">Nanomaterials (2079-4991)</searchLink>. Mar2024, Vol. 14 Issue 5, p453. 11p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Oligonucleotides%22">Oligonucleotides</searchLink><br /><searchLink fieldCode="DE" term="%22Nanowires%22">Nanowires</searchLink><br /><searchLink fieldCode="DE" term="%22Light+cones%22">Light cones</searchLink><br /><searchLink fieldCode="DE" term="%22Signal-to-noise+ratio%22">Signal-to-noise ratio</searchLink><br /><searchLink fieldCode="DE" term="%22Detection+limit%22">Detection limit</searchLink><br /><searchLink fieldCode="DE" term="%22Emission+standards%22">Emission standards</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The detection of oligonucleotides is a central step in many biomedical investigations. The most commonly used methods for detecting oligonucleotides often require concentration and amplification before detection. Therefore, developing detection methods with a direct read-out would be beneficial. Although commonly used for the detection of amplified oligonucleotides, fluorescent molecular beacons have been proposed for such direct detection. However, the reported limits of detection using molecular beacons are relatively high, ranging from 100 nM to a few µM, primarily limited by the beacon fluorescence background. In this study, we enhanced the relative signal contrast between hybridized and non-hybridized states of the beacons by immobilizing them on lightguiding nanowires. Upon hybridization to a complementary oligonucleotide, the fluorescence from the surface-bound beacon becomes coupled in the lightguiding nanowire core and is re-emitted at the nanowire tip in a narrower cone of light compared with the standard 4π emission. Prior knowledge of the nanowire positions allows for the continuous monitoring of fluorescence signals from each nanowire, which effectively facilitates the discrimination of signals arising from hybridization events against background signals. This resulted in improved signal-to-background and signal-to-noise ratios, which allowed for the direct detection of oligonucleotides at a concentration as low as 0.1 nM. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=175991968 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3390/nano14050453 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 453 Subjects: – SubjectFull: Oligonucleotides Type: general – SubjectFull: Nanowires Type: general – SubjectFull: Light cones Type: general – SubjectFull: Signal-to-noise ratio Type: general – SubjectFull: Detection limit Type: general – SubjectFull: Emission standards Type: general Titles: – TitleFull: Sub-Nanomolar Detection of Oligonucleotides Using Molecular Beacons Immobilized on Lightguiding Nanowires. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Johansson, Therese B. – PersonEntity: Name: NameFull: Davtyan, Rubina – PersonEntity: Name: NameFull: Valderas-Gutiérrez, Julia – PersonEntity: Name: NameFull: Gonzalez Rodriguez, Adrian – PersonEntity: Name: NameFull: Agnarsson, Björn – PersonEntity: Name: NameFull: Munita, Roberto – PersonEntity: Name: NameFull: Fioretos, Thoas – PersonEntity: Name: NameFull: Lilljebjörn, Henrik – PersonEntity: Name: NameFull: Linke, Heiner – PersonEntity: Name: NameFull: Höök, Fredrik – PersonEntity: Name: NameFull: Prinz, Christelle N. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: Mar2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 20794991 Numbering: – Type: volume Value: 14 – Type: issue Value: 5 Titles: – TitleFull: Nanomaterials (2079-4991) Type: main |
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