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Extraction, purification, and investigation of the antibacterial potential of lysozyme isolated from the latex of Calotropis procera.

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Title: Extraction, purification, and investigation of the antibacterial potential of lysozyme isolated from the latex of Calotropis procera.
Authors: Muthu, Sakthivel1,2 (AUTHOR) saktthivel@gmail.com, Gopal, Venkateshbabu1 (AUTHOR), AL-Younis, Zena Kadhim3 (AUTHOR), Altemimi, Ammar B.3,4 (AUTHOR), Perumal, Palani1 (AUTHOR), Lakshmikanthan, Mythileeswari2 (AUTHOR), Krishnan, Kathiravan2 (AUTHOR), Najm, Mazin A. A.5 (AUTHOR), Giuffrè, Angelo Maria6 (AUTHOR) amgiuffre@unirc.it, Govindan, Lakshmanan7 (AUTHOR)
Source: European Food Research & Technology. May2024, Vol. 250 Issue 5, p1401-1416. 16p.
Subjects: Calotropis procera, Lysozymes, Gel permeation chromatography, Latex, Escherichia coli, Analytical chemistry
Abstract: Calotropis procera produces a latex used in traditional medicine because of its components which are found to be biologically active. The latex of C. procera has many benefits such as pain reduction, antimicrobial attitude and other precious attributes. Latex lysozyme (LL) is a particular protein contained in C. procera plant having a promising antibacterial property. The process applied for extraction implicated ammonium sulfate precipitation, water extraction, ion exchange and gel filtration column chromatography evidencing 14.4 kDa for LL as a molecular mass. LL after purification showed 397.18 U mg−1 as specific activity (10.30 purification fold), and with 37.9% as a yield. In addition, the chemical and physical analyses showed that LL had its best realization when the pH was 5.5 at 50 °C. Moreover when metal ions such as Ca2+ and Mg2+ were incorporated the relative activity of the enzyme resulted ampliflied. When a purification was conducted with CM-Cellulose and Sephadex G-100 chromatography, LL revealed an antibacterial property versus both Escherichia coli (MICs 14 µg ml−1) and Bacillus cereus (MICs 13 µg ml−1). HR-TEM analysis displayed an antimicrobial potential of LL after its purification; that analysis showed the deterioration of the cell wall (external membrane of the cell) of both E. coli and B. cereus. Therefore, the LL obtained from C. procera maintains ability as an antibacterial enzyme applicable in various domains such as biotechnological and pharmaceutical industries. [ABSTRACT FROM AUTHOR]
Copyright of European Food Research & Technology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Extraction, purification, and investigation of the antibacterial potential of lysozyme isolated from the latex of Calotropis procera.
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  Data: <searchLink fieldCode="AR" term="%22Muthu%2C+Sakthivel%22">Muthu, Sakthivel</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> saktthivel@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Gopal%2C+Venkateshbabu%22">Gopal, Venkateshbabu</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22AL-Younis%2C+Zena+Kadhim%22">AL-Younis, Zena Kadhim</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Altemimi%2C+Ammar+B%2E%22">Altemimi, Ammar B.</searchLink><relatesTo>3,4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Perumal%2C+Palani%22">Perumal, Palani</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lakshmikanthan%2C+Mythileeswari%22">Lakshmikanthan, Mythileeswari</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Krishnan%2C+Kathiravan%22">Krishnan, Kathiravan</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Najm%2C+Mazin+A%2E+A%2E%22">Najm, Mazin A. A.</searchLink><relatesTo>5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Giuffrè%2C+Angelo+Maria%22">Giuffrè, Angelo Maria</searchLink><relatesTo>6</relatesTo> (AUTHOR)<i> amgiuffre@unirc.it</i><br /><searchLink fieldCode="AR" term="%22Govindan%2C+Lakshmanan%22">Govindan, Lakshmanan</searchLink><relatesTo>7</relatesTo> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22European+Food+Research+%26+Technology%22">European Food Research & Technology</searchLink>. May2024, Vol. 250 Issue 5, p1401-1416. 16p.
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  Data: <searchLink fieldCode="DE" term="%22Calotropis+procera%22">Calotropis procera</searchLink><br /><searchLink fieldCode="DE" term="%22Lysozymes%22">Lysozymes</searchLink><br /><searchLink fieldCode="DE" term="%22Gel+permeation+chromatography%22">Gel permeation chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Latex%22">Latex</searchLink><br /><searchLink fieldCode="DE" term="%22Escherichia+coli%22">Escherichia coli</searchLink><br /><searchLink fieldCode="DE" term="%22Analytical+chemistry%22">Analytical chemistry</searchLink>
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  Data: Calotropis procera produces a latex used in traditional medicine because of its components which are found to be biologically active. The latex of C. procera has many benefits such as pain reduction, antimicrobial attitude and other precious attributes. Latex lysozyme (LL) is a particular protein contained in C. procera plant having a promising antibacterial property. The process applied for extraction implicated ammonium sulfate precipitation, water extraction, ion exchange and gel filtration column chromatography evidencing 14.4 kDa for LL as a molecular mass. LL after purification showed 397.18 U mg−1 as specific activity (10.30 purification fold), and with 37.9% as a yield. In addition, the chemical and physical analyses showed that LL had its best realization when the pH was 5.5 at 50 °C. Moreover when metal ions such as Ca2+ and Mg2+ were incorporated the relative activity of the enzyme resulted ampliflied. When a purification was conducted with CM-Cellulose and Sephadex G-100 chromatography, LL revealed an antibacterial property versus both Escherichia coli (MICs 14 µg ml−1) and Bacillus cereus (MICs 13 µg ml−1). HR-TEM analysis displayed an antimicrobial potential of LL after its purification; that analysis showed the deterioration of the cell wall (external membrane of the cell) of both E. coli and B. cereus. Therefore, the LL obtained from C. procera maintains ability as an antibacterial enzyme applicable in various domains such as biotechnological and pharmaceutical industries. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of European Food Research & Technology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1007/s00217-024-04467-2
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      – Code: eng
        Text: English
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        PageCount: 16
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    Subjects:
      – SubjectFull: Calotropis procera
        Type: general
      – SubjectFull: Lysozymes
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      – SubjectFull: Gel permeation chromatography
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              Text: May2024
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