Ploidy‐specific transcriptomes shed light on the heterogeneous identity and metabolism of developing tomato pericarp cells.
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| Title: | Ploidy‐specific transcriptomes shed light on the heterogeneous identity and metabolism of developing tomato pericarp cells. |
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| Authors: | Tourdot, Edouard1 (AUTHOR), Martin, Pascal G. P.1 (AUTHOR), Maza, Elie2 (AUTHOR), Mauxion, Jean‐Philippe1 (AUTHOR), Djari, Anis2 (AUTHOR), Gévaudant, Frédéric1 (AUTHOR), Chevalier, Christian1 (AUTHOR), Pirrello, Julien2 (AUTHOR), Gonzalez, Nathalie1 (AUTHOR) nathalie.gonzalez@inrae.fr |
| Source: | Plant Journal. May2024, Vol. 118 Issue 4, p997-1015. 19p. |
| Subjects: | Pericarp, Tomatoes, Transcriptomes, Gene expression, Fruit development, Ploidy |
| Abstract: | SUMMARY: Endoreduplication, during which cells increase their DNA content through successive rounds of full genome replication without cell division, is the major source of endopolyploidy in higher plants. Endoreduplication plays pivotal roles in plant growth and development and is associated with the activation of specific transcriptional programmes that are characteristic of each cell type, thereby defining their identity. In plants, endoreduplication is found in numerous organs and cell types, especially in agronomically valuable ones, such as the fleshy fruit (pericarp) of tomato presenting high ploidy levels. We used the tomato pericarp tissue as a model system to explore the transcriptomes associated with endoreduplication progression during fruit growth. We confirmed that expression globally scales with ploidy level and identified sets of differentially expressed genes presenting only developmental‐specific, only ploidy‐specific expression patterns or profiles resulting from an additive effect of ploidy and development. When comparing ploidy levels at a specific developmental stage, we found that non‐endoreduplicated cells are defined by cell division state and cuticle synthesis while endoreduplicated cells are mainly defined by their metabolic activity changing rapidly over time. By combining this dataset with publicly available spatiotemporal pericarp expression data, we proposed a map describing the distribution of ploidy levels within the pericarp. These transcriptome‐based predictions were validated by quantifying ploidy levels within the pericarp tissue. This in situ ploidy quantification revealed the dynamic progression of endoreduplication and its cell layer specificity during early fruit development. In summary, the study sheds light on the complex relationship between endoreduplication, cell differentiation and gene expression patterns in the tomato pericarp. [ABSTRACT FROM AUTHOR] |
| Copyright of Plant Journal is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 177219495 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Ploidy‐specific transcriptomes shed light on the heterogeneous identity and metabolism of developing tomato pericarp cells. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Tourdot%2C+Edouard%22">Tourdot, Edouard</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Martin%2C+Pascal+G%2E+P%2E%22">Martin, Pascal G. P.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Maza%2C+Elie%22">Maza, Elie</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mauxion%2C+Jean‐Philippe%22">Mauxion, Jean‐Philippe</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Djari%2C+Anis%22">Djari, Anis</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Gévaudant%2C+Frédéric%22">Gévaudant, Frédéric</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chevalier%2C+Christian%22">Chevalier, Christian</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pirrello%2C+Julien%22">Pirrello, Julien</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Gonzalez%2C+Nathalie%22">Gonzalez, Nathalie</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> nathalie.gonzalez@inrae.fr</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Plant+Journal%22">Plant Journal</searchLink>. May2024, Vol. 118 Issue 4, p997-1015. 19p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Pericarp%22">Pericarp</searchLink><br /><searchLink fieldCode="DE" term="%22Tomatoes%22">Tomatoes</searchLink><br /><searchLink fieldCode="DE" term="%22Transcriptomes%22">Transcriptomes</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Fruit+development%22">Fruit development</searchLink><br /><searchLink fieldCode="DE" term="%22Ploidy%22">Ploidy</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: SUMMARY: Endoreduplication, during which cells increase their DNA content through successive rounds of full genome replication without cell division, is the major source of endopolyploidy in higher plants. Endoreduplication plays pivotal roles in plant growth and development and is associated with the activation of specific transcriptional programmes that are characteristic of each cell type, thereby defining their identity. In plants, endoreduplication is found in numerous organs and cell types, especially in agronomically valuable ones, such as the fleshy fruit (pericarp) of tomato presenting high ploidy levels. We used the tomato pericarp tissue as a model system to explore the transcriptomes associated with endoreduplication progression during fruit growth. We confirmed that expression globally scales with ploidy level and identified sets of differentially expressed genes presenting only developmental‐specific, only ploidy‐specific expression patterns or profiles resulting from an additive effect of ploidy and development. When comparing ploidy levels at a specific developmental stage, we found that non‐endoreduplicated cells are defined by cell division state and cuticle synthesis while endoreduplicated cells are mainly defined by their metabolic activity changing rapidly over time. By combining this dataset with publicly available spatiotemporal pericarp expression data, we proposed a map describing the distribution of ploidy levels within the pericarp. These transcriptome‐based predictions were validated by quantifying ploidy levels within the pericarp tissue. This in situ ploidy quantification revealed the dynamic progression of endoreduplication and its cell layer specificity during early fruit development. In summary, the study sheds light on the complex relationship between endoreduplication, cell differentiation and gene expression patterns in the tomato pericarp. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Plant Journal is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=177219495 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1111/tpj.16646 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 19 StartPage: 997 Subjects: – SubjectFull: Pericarp Type: general – SubjectFull: Tomatoes Type: general – SubjectFull: Transcriptomes Type: general – SubjectFull: Gene expression Type: general – SubjectFull: Fruit development Type: general – SubjectFull: Ploidy Type: general Titles: – TitleFull: Ploidy‐specific transcriptomes shed light on the heterogeneous identity and metabolism of developing tomato pericarp cells. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Tourdot, Edouard – PersonEntity: Name: NameFull: Martin, Pascal G. P. – PersonEntity: Name: NameFull: Maza, Elie – PersonEntity: Name: NameFull: Mauxion, Jean‐Philippe – PersonEntity: Name: NameFull: Djari, Anis – PersonEntity: Name: NameFull: Gévaudant, Frédéric – PersonEntity: Name: NameFull: Chevalier, Christian – PersonEntity: Name: NameFull: Pirrello, Julien – PersonEntity: Name: NameFull: Gonzalez, Nathalie IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 05 Text: May2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 09607412 Numbering: – Type: volume Value: 118 – Type: issue Value: 4 Titles: – TitleFull: Plant Journal Type: main |
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