View in EDS

Exploring associations between the teat apex metagenome and Staphylococcus aureus intramammary infections in primiparous cows under organic directives.

Saved in:
Bibliographic Details
Title: Exploring associations between the teat apex metagenome and Staphylococcus aureus intramammary infections in primiparous cows under organic directives.
Authors: Dean, C. J.1, Peña-Mosca, F.1, Ray, T.1, Wehri, T. J.2, Sharpe, K.2, Antunes Jr., A. M.1, Doster, E.1, Fernandes, L.3, Calles, V. F.1, Bauman, C.1, Godden, S.1, Heins, B.2, Pinedo, P.4, Machado, V. S.3, Caixeta, L. S.1, Noyes, N. R.1 nnoyes@umn.edu
Source: Applied & Environmental Microbiology. Apr2024, Vol. 90 Issue 4, p1-23. 23p.
Subjects: Staphylococcus aureus infections, Organic dairy farming, Staphylococcus aureus, Cows, Antimicrobial peptides, Dairy industry
Abstract: The primary objective of this study was to identify associations between the prepartum teat apex microbiome and the presence of Staphylococcus aureus intramammary infections (IMI) in primiparous cows during the first 5 weeks after calving. We performed a case-control study using shotgun metagenomics of the teat apex and culture-based milk data collected longitudinally from 710 primiparous cows on five organic dairy farms. Cases had higher odds of having S. aureus metagenomic DNA on the teat apex prior to parturition compared to controls (OR = 38.9, 95% CI: 14.84-102.21). Differential abundance analysis confirmed this association, with cases having a 23.8 higher log fold change (LFC) in the abundance of S. aureus in their samples compared to controls. Of the most prevalent microorganisms in controls, those associated with a lower risk of post-calving S. aureus IMI included Microbacterium phage Min 1 (OR = 0.37, 95% CI: 0.25-0.53), Corynebacterium efficiens (OR = 0.53, 95% CI: 0.30-0.94), Kocuria polaris (OR = 0.54, 95% CI: 0.35-0.82), Micrococcus terreus (OR = 0.64, 95% CI: 0.44-0.93), and Dietzia alimentaria (OR = 0.45, 95% CI: 0.26-0.75). Genes encoding for Microcin B17 AMPs were the most prevalent on the teat apex of cases and controls (99.7% in both groups). The predicted abundance of genes encoding for Microcin B17 was also higher in cases compared to controls (LFC 0.26). IMPORTANCE Intramammary infections (IMI) caused by Staphylococcus aureus remain an important problem for the dairy industry. The microbiome on the external skin of the teat apex may play a role in mitigating S. aureus IMI risk, in particular the production of antimicrobial peptides (AMPs) by commensal microbes. However, current studies of the teat apex microbiome utilize a 16S approach, which precludes the detection of genomic features such as genes that encode for AMPs. Therefore, further research using a shotgun metagenomic approach is needed to understand what role prepartum teat apex microbiome dynamics play in IMI risk. [ABSTRACT FROM AUTHOR]
Copyright of Applied & Environmental Microbiology is the property of American Society for Microbiology and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 177256381
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Exploring associations between the teat apex metagenome and Staphylococcus aureus intramammary infections in primiparous cows under organic directives.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Dean%2C+C%2E+J%2E%22">Dean, C. J.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Peña-Mosca%2C+F%2E%22">Peña-Mosca, F.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Ray%2C+T%2E%22">Ray, T.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wehri%2C+T%2E+J%2E%22">Wehri, T. J.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Sharpe%2C+K%2E%22">Sharpe, K.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Antunes+Jr%2E%2C+A%2E+M%2E%22">Antunes Jr., A. M.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Doster%2C+E%2E%22">Doster, E.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Fernandes%2C+L%2E%22">Fernandes, L.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Calles%2C+V%2E+F%2E%22">Calles, V. F.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Bauman%2C+C%2E%22">Bauman, C.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Godden%2C+S%2E%22">Godden, S.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Heins%2C+B%2E%22">Heins, B.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Pinedo%2C+P%2E%22">Pinedo, P.</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Machado%2C+V%2E+S%2E%22">Machado, V. S.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Caixeta%2C+L%2E+S%2E%22">Caixeta, L. S.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Noyes%2C+N%2E+R%2E%22">Noyes, N. R.</searchLink><relatesTo>1</relatesTo><i> nnoyes@umn.edu</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Applied+%26+Environmental+Microbiology%22">Applied & Environmental Microbiology</searchLink>. Apr2024, Vol. 90 Issue 4, p1-23. 23p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Staphylococcus+aureus+infections%22">Staphylococcus aureus infections</searchLink><br /><searchLink fieldCode="DE" term="%22Organic+dairy+farming%22">Organic dairy farming</searchLink><br /><searchLink fieldCode="DE" term="%22Staphylococcus+aureus%22">Staphylococcus aureus</searchLink><br /><searchLink fieldCode="DE" term="%22Cows%22">Cows</searchLink><br /><searchLink fieldCode="DE" term="%22Antimicrobial+peptides%22">Antimicrobial peptides</searchLink><br /><searchLink fieldCode="DE" term="%22Dairy+industry%22">Dairy industry</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The primary objective of this study was to identify associations between the prepartum teat apex microbiome and the presence of Staphylococcus aureus intramammary infections (IMI) in primiparous cows during the first 5 weeks after calving. We performed a case-control study using shotgun metagenomics of the teat apex and culture-based milk data collected longitudinally from 710 primiparous cows on five organic dairy farms. Cases had higher odds of having S. aureus metagenomic DNA on the teat apex prior to parturition compared to controls (OR = 38.9, 95% CI: 14.84-102.21). Differential abundance analysis confirmed this association, with cases having a 23.8 higher log fold change (LFC) in the abundance of S. aureus in their samples compared to controls. Of the most prevalent microorganisms in controls, those associated with a lower risk of post-calving S. aureus IMI included Microbacterium phage Min 1 (OR = 0.37, 95% CI: 0.25-0.53), Corynebacterium efficiens (OR = 0.53, 95% CI: 0.30-0.94), Kocuria polaris (OR = 0.54, 95% CI: 0.35-0.82), Micrococcus terreus (OR = 0.64, 95% CI: 0.44-0.93), and Dietzia alimentaria (OR = 0.45, 95% CI: 0.26-0.75). Genes encoding for Microcin B17 AMPs were the most prevalent on the teat apex of cases and controls (99.7% in both groups). The predicted abundance of genes encoding for Microcin B17 was also higher in cases compared to controls (LFC 0.26). IMPORTANCE Intramammary infections (IMI) caused by Staphylococcus aureus remain an important problem for the dairy industry. The microbiome on the external skin of the teat apex may play a role in mitigating S. aureus IMI risk, in particular the production of antimicrobial peptides (AMPs) by commensal microbes. However, current studies of the teat apex microbiome utilize a 16S approach, which precludes the detection of genomic features such as genes that encode for AMPs. Therefore, further research using a shotgun metagenomic approach is needed to understand what role prepartum teat apex microbiome dynamics play in IMI risk. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Applied & Environmental Microbiology is the property of American Society for Microbiology and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=177256381
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1128/aem.02234-23
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 23
        StartPage: 1
    Subjects:
      – SubjectFull: Staphylococcus aureus infections
        Type: general
      – SubjectFull: Organic dairy farming
        Type: general
      – SubjectFull: Staphylococcus aureus
        Type: general
      – SubjectFull: Cows
        Type: general
      – SubjectFull: Antimicrobial peptides
        Type: general
      – SubjectFull: Dairy industry
        Type: general
    Titles:
      – TitleFull: Exploring associations between the teat apex metagenome and Staphylococcus aureus intramammary infections in primiparous cows under organic directives.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Dean, C. J.
      – PersonEntity:
          Name:
            NameFull: Peña-Mosca, F.
      – PersonEntity:
          Name:
            NameFull: Ray, T.
      – PersonEntity:
          Name:
            NameFull: Wehri, T. J.
      – PersonEntity:
          Name:
            NameFull: Sharpe, K.
      – PersonEntity:
          Name:
            NameFull: Antunes Jr., A. M.
      – PersonEntity:
          Name:
            NameFull: Doster, E.
      – PersonEntity:
          Name:
            NameFull: Fernandes, L.
      – PersonEntity:
          Name:
            NameFull: Calles, V. F.
      – PersonEntity:
          Name:
            NameFull: Bauman, C.
      – PersonEntity:
          Name:
            NameFull: Godden, S.
      – PersonEntity:
          Name:
            NameFull: Heins, B.
      – PersonEntity:
          Name:
            NameFull: Pinedo, P.
      – PersonEntity:
          Name:
            NameFull: Machado, V. S.
      – PersonEntity:
          Name:
            NameFull: Caixeta, L. S.
      – PersonEntity:
          Name:
            NameFull: Noyes, N. R.
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 04
              Text: Apr2024
              Type: published
              Y: 2024
          Identifiers:
            – Type: issn-print
              Value: 00992240
          Numbering:
            – Type: volume
              Value: 90
            – Type: issue
              Value: 4
          Titles:
            – TitleFull: Applied & Environmental Microbiology
              Type: main
ResultId 1