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Coaggregated E. faecalis with F. nucleatum regulated environmental stress responses and inflammatory effects.

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Title: Coaggregated E. faecalis with F. nucleatum regulated environmental stress responses and inflammatory effects.
Authors: Zhou, Jiani1,2 (AUTHOR), Yuan, Zijian1,2 (AUTHOR), Yang, Ruiqi1,2 (AUTHOR), Liu, Tingjun1,2 (AUTHOR), Lu, Xianjun1,2 (AUTHOR), Huang, Wenling1,2 (AUTHOR), Guo, Lihong1,2 (AUTHOR) guolh5@mail.sysu.edu.cn
Source: Applied Microbiology & Biotechnology. 5/18/2024, Vol. 108 Issue 1, p1-21. 21p.
Subjects: Cell communication, Inflammation, Enterococcus faecalis, Quorum sensing, Dental pulp cavities, Cellular signal transduction, Lysine, Arginine
Abstract: To investigate the cell-cell interactions of intergeneric bacterial species, the study detected the survival of Enterococcus faecalis (Ef) under monospecies or coaggregation state with Fusobacterium nucleatum subsp. polymorphum (Fnp) in environmental stress. Ef and Fnp infected the human macrophages with different forms (Ef and Fnp monospecies, Ef-Fnp coaggregates, Ef + Fnp cocultures) for exploring the immunoregulatory effects and the relevant molecular mechanisms. Meanwhile, the transcriptomic profiles of coaggregated Ef and Fnp were analyzed. Ef was shown to coaggregate with Fnp strongly in CAB within 90 min by forming multiplexes clumps. Coaggregation with Fnp reinforced Ef resistance against unfavorable conditions including alkaline, hypertonic, nutrient-starvation, and antibiotic challenges. Compared with monospecies and coculture species, the coaggregation of Ef and Fnp significantly facilitates both species to invade dTHP-1 cells and aid Ef to survive within the cells. Compared with coculture species, dual-species interaction of Ef and Fnp significantly decreased the levels of pro-inflammatory cytokines IL-6, TNF-α, and chemokines MCP-1 secreted by dTHP-1 cells and lessened the phosphorylation of p38, JNK, and p65 signaling pathways. The transcriptome sequencing results showed that 111 genes were differentially expressed or Ef-Fnp coaggregated species compared to Ef monospecies; 651 genes were differentially expressed for Fnp when coaggregation with Ef. The analysis of KEGG pathway showed that Ef differentially expressed genes (DEGs) were enriched in quorum sensing and arginine biosynthesis pathway; Fnp DEGs were differentially concentrated in lipopolysaccharide (LPS) biosynthesis, biofilm formation, and lysine degradation pathway compared to monospecies. Key points: • Coaggregated with Fnp aids Ef's survival in environmental stress, especially in root canals after endodontic treatment. • The coaggregation of Ef and Fnp may weaken the pro-inflammatory response and facilitate Ef to evade killed by macrophages. • The coaggregation between Ef and Fnp altered interspecies transcriptional profiles. [ABSTRACT FROM AUTHOR]
Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Coaggregated E. faecalis with F. nucleatum regulated environmental stress responses and inflammatory effects.
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  Data: <searchLink fieldCode="AR" term="%22Zhou%2C+Jiani%22">Zhou, Jiani</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yuan%2C+Zijian%22">Yuan, Zijian</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yang%2C+Ruiqi%22">Yang, Ruiqi</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Liu%2C+Tingjun%22">Liu, Tingjun</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lu%2C+Xianjun%22">Lu, Xianjun</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Huang%2C+Wenling%22">Huang, Wenling</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Guo%2C+Lihong%22">Guo, Lihong</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> guolh5@mail.sysu.edu.cn</i>
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  Data: <searchLink fieldCode="JN" term="%22Applied+Microbiology+%26+Biotechnology%22">Applied Microbiology & Biotechnology</searchLink>. 5/18/2024, Vol. 108 Issue 1, p1-21. 21p.
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  Data: <searchLink fieldCode="DE" term="%22Cell+communication%22">Cell communication</searchLink><br /><searchLink fieldCode="DE" term="%22Inflammation%22">Inflammation</searchLink><br /><searchLink fieldCode="DE" term="%22Enterococcus+faecalis%22">Enterococcus faecalis</searchLink><br /><searchLink fieldCode="DE" term="%22Quorum+sensing%22">Quorum sensing</searchLink><br /><searchLink fieldCode="DE" term="%22Dental+pulp+cavities%22">Dental pulp cavities</searchLink><br /><searchLink fieldCode="DE" term="%22Cellular+signal+transduction%22">Cellular signal transduction</searchLink><br /><searchLink fieldCode="DE" term="%22Lysine%22">Lysine</searchLink><br /><searchLink fieldCode="DE" term="%22Arginine%22">Arginine</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: To investigate the cell-cell interactions of intergeneric bacterial species, the study detected the survival of Enterococcus faecalis (Ef) under monospecies or coaggregation state with Fusobacterium nucleatum subsp. polymorphum (Fnp) in environmental stress. Ef and Fnp infected the human macrophages with different forms (Ef and Fnp monospecies, Ef-Fnp coaggregates, Ef + Fnp cocultures) for exploring the immunoregulatory effects and the relevant molecular mechanisms. Meanwhile, the transcriptomic profiles of coaggregated Ef and Fnp were analyzed. Ef was shown to coaggregate with Fnp strongly in CAB within 90 min by forming multiplexes clumps. Coaggregation with Fnp reinforced Ef resistance against unfavorable conditions including alkaline, hypertonic, nutrient-starvation, and antibiotic challenges. Compared with monospecies and coculture species, the coaggregation of Ef and Fnp significantly facilitates both species to invade dTHP-1 cells and aid Ef to survive within the cells. Compared with coculture species, dual-species interaction of Ef and Fnp significantly decreased the levels of pro-inflammatory cytokines IL-6, TNF-α, and chemokines MCP-1 secreted by dTHP-1 cells and lessened the phosphorylation of p38, JNK, and p65 signaling pathways. The transcriptome sequencing results showed that 111 genes were differentially expressed or Ef-Fnp coaggregated species compared to Ef monospecies; 651 genes were differentially expressed for Fnp when coaggregation with Ef. The analysis of KEGG pathway showed that Ef differentially expressed genes (DEGs) were enriched in quorum sensing and arginine biosynthesis pathway; Fnp DEGs were differentially concentrated in lipopolysaccharide (LPS) biosynthesis, biofilm formation, and lysine degradation pathway compared to monospecies. Key points: • Coaggregated with Fnp aids Ef's survival in environmental stress, especially in root canals after endodontic treatment. • The coaggregation of Ef and Fnp may weaken the pro-inflammatory response and facilitate Ef to evade killed by macrophages. • The coaggregation between Ef and Fnp altered interspecies transcriptional profiles. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1007/s00253-024-13172-9
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      – Code: eng
        Text: English
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        PageCount: 21
        StartPage: 1
    Subjects:
      – SubjectFull: Cell communication
        Type: general
      – SubjectFull: Inflammation
        Type: general
      – SubjectFull: Enterococcus faecalis
        Type: general
      – SubjectFull: Quorum sensing
        Type: general
      – SubjectFull: Dental pulp cavities
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      – SubjectFull: Cellular signal transduction
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      – SubjectFull: Lysine
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      – SubjectFull: Arginine
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      – TitleFull: Coaggregated E. faecalis with F. nucleatum regulated environmental stress responses and inflammatory effects.
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              M: 05
              Text: 5/18/2024
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              Y: 2024
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