View in EDS HTML Full Text PDF Full Text

Advanced detection of cervical cancer biomarkers using engineered filamentous phage nanofibers.

Saved in:
Bibliographic Details
Title: Advanced detection of cervical cancer biomarkers using engineered filamentous phage nanofibers.
Authors: Zhou, Xu1 (AUTHOR), Wang, Yicun2 (AUTHOR) wangyicun@jlu.edu.cn, Bao, Meijing1 (AUTHOR), Chu, Yuqing1 (AUTHOR), Liu, Ruixue1 (AUTHOR), Chen, Qi1 (AUTHOR), Lin, Yang1 (AUTHOR) linyang@jlu.edu.cn
Source: Applied Microbiology & Biotechnology. 2/19/2024, Vol. 108 Issue 1, p1-10. 10p.
Subjects: Cervical cancer, Papillomaviruses, Programmed death-ligand 1, Bacteriophages, Enzyme-linked immunosorbent assay, Vascular endothelial growth factors, Tumor markers, Nanofibers, Early detection of cancer
Abstract: Cervical cancer is a major global health concern, characterized by its high incidence and mortality rates. The detection of tumor markers is crucial for managing cancer, making treatment decisions, and monitoring disease progression. Vascular endothelial growth factor (VEGF) and programmed death-ligand 1 (PDL-1) are key targets in cervical cancer therapy and valuable biomarkers in predicting treatment response and prognosis. In this study, we found that combining the measurement of VEGF and soluble PDL-1 can be used for diagnosing and evaluating the progression of cervical cancer. To explore a more convenient approach for detecting and assessing cervical cancer, we designed and prepared an engineered fd bacteriophage, a human-safe viral nanofiber, equipped with two peptides targeting VEGF and PD-L1. The dual-display phage nanofiber specifically recognizes and binds to both proteins. Utilizing this nanofiber as a novel capture agent, we developed a new enzyme-linked immunosorbent assay (ELISA) method. This method shows significantly enhanced detection sensitivity compared to conventional ELISA methods, which use either anti-VEGF or anti-PD-L1 antibodies as capture agents. Therefore, the phage dual-display nanofiber presents significant potential in detecting cancer markers, evaluating medication efficacy, and advancing immunotherapy drug development. Key points: • The combined measurement of VEGF and soluble Programmed Death-Ligand 1(sPD-L1) demonstrates an additive effect in the diagnosis of cervical cancer. Fd phage nanofibers have been ingeniously engineered to display peptides that bind to VEGF and PD-L1, enabling the simultaneous detection of both proteins within a single assay • Genetically engineered phage nanofibers, adorned with two distinct peptides, can be utilized for the diagnosis and prognosis of cancer and can be mass-produced cost-effectively through bacterial infections • Employing dual-display fd phage nanofibers as capture probes, the phage ELISA method exhibited significantly enhanced detection sensitivity compared to traditional sandwich ELISA. Furthermore, phage ELISA facilitates the detection of a single protein or the simultaneous detection of multiple proteins, rendering them powerful tools for protein analysis and diagnosis across various fields, including cancer research [ABSTRACT FROM AUTHOR]
Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Full text is not displayed to guests.
FullText Links:
  – Type: pdflink
Text:
  Availability: 1
Header DbId: egs
DbLabel: Engineering Source
An: 177880036
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Advanced detection of cervical cancer biomarkers using engineered filamentous phage nanofibers.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Zhou%2C+Xu%22">Zhou, Xu</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Yicun%22">Wang, Yicun</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> wangyicun@jlu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Bao%2C+Meijing%22">Bao, Meijing</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chu%2C+Yuqing%22">Chu, Yuqing</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Liu%2C+Ruixue%22">Liu, Ruixue</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chen%2C+Qi%22">Chen, Qi</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lin%2C+Yang%22">Lin, Yang</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> linyang@jlu.edu.cn</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Applied+Microbiology+%26+Biotechnology%22">Applied Microbiology & Biotechnology</searchLink>. 2/19/2024, Vol. 108 Issue 1, p1-10. 10p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Cervical+cancer%22">Cervical cancer</searchLink><br /><searchLink fieldCode="DE" term="%22Papillomaviruses%22">Papillomaviruses</searchLink><br /><searchLink fieldCode="DE" term="%22Programmed+death-ligand+1%22">Programmed death-ligand 1</searchLink><br /><searchLink fieldCode="DE" term="%22Bacteriophages%22">Bacteriophages</searchLink><br /><searchLink fieldCode="DE" term="%22Enzyme-linked+immunosorbent+assay%22">Enzyme-linked immunosorbent assay</searchLink><br /><searchLink fieldCode="DE" term="%22Vascular+endothelial+growth+factors%22">Vascular endothelial growth factors</searchLink><br /><searchLink fieldCode="DE" term="%22Tumor+markers%22">Tumor markers</searchLink><br /><searchLink fieldCode="DE" term="%22Nanofibers%22">Nanofibers</searchLink><br /><searchLink fieldCode="DE" term="%22Early+detection+of+cancer%22">Early detection of cancer</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Cervical cancer is a major global health concern, characterized by its high incidence and mortality rates. The detection of tumor markers is crucial for managing cancer, making treatment decisions, and monitoring disease progression. Vascular endothelial growth factor (VEGF) and programmed death-ligand 1 (PDL-1) are key targets in cervical cancer therapy and valuable biomarkers in predicting treatment response and prognosis. In this study, we found that combining the measurement of VEGF and soluble PDL-1 can be used for diagnosing and evaluating the progression of cervical cancer. To explore a more convenient approach for detecting and assessing cervical cancer, we designed and prepared an engineered fd bacteriophage, a human-safe viral nanofiber, equipped with two peptides targeting VEGF and PD-L1. The dual-display phage nanofiber specifically recognizes and binds to both proteins. Utilizing this nanofiber as a novel capture agent, we developed a new enzyme-linked immunosorbent assay (ELISA) method. This method shows significantly enhanced detection sensitivity compared to conventional ELISA methods, which use either anti-VEGF or anti-PD-L1 antibodies as capture agents. Therefore, the phage dual-display nanofiber presents significant potential in detecting cancer markers, evaluating medication efficacy, and advancing immunotherapy drug development. Key points: • The combined measurement of VEGF and soluble Programmed Death-Ligand 1(sPD-L1) demonstrates an additive effect in the diagnosis of cervical cancer. Fd phage nanofibers have been ingeniously engineered to display peptides that bind to VEGF and PD-L1, enabling the simultaneous detection of both proteins within a single assay • Genetically engineered phage nanofibers, adorned with two distinct peptides, can be utilized for the diagnosis and prognosis of cancer and can be mass-produced cost-effectively through bacterial infections • Employing dual-display fd phage nanofibers as capture probes, the phage ELISA method exhibited significantly enhanced detection sensitivity compared to traditional sandwich ELISA. Furthermore, phage ELISA facilitates the detection of a single protein or the simultaneous detection of multiple proteins, rendering them powerful tools for protein analysis and diagnosis across various fields, including cancer research [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=177880036
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1007/s00253-024-13058-w
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 10
        StartPage: 1
    Subjects:
      – SubjectFull: Cervical cancer
        Type: general
      – SubjectFull: Papillomaviruses
        Type: general
      – SubjectFull: Programmed death-ligand 1
        Type: general
      – SubjectFull: Bacteriophages
        Type: general
      – SubjectFull: Enzyme-linked immunosorbent assay
        Type: general
      – SubjectFull: Vascular endothelial growth factors
        Type: general
      – SubjectFull: Tumor markers
        Type: general
      – SubjectFull: Nanofibers
        Type: general
      – SubjectFull: Early detection of cancer
        Type: general
    Titles:
      – TitleFull: Advanced detection of cervical cancer biomarkers using engineered filamentous phage nanofibers.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Zhou, Xu
      – PersonEntity:
          Name:
            NameFull: Wang, Yicun
      – PersonEntity:
          Name:
            NameFull: Bao, Meijing
      – PersonEntity:
          Name:
            NameFull: Chu, Yuqing
      – PersonEntity:
          Name:
            NameFull: Liu, Ruixue
      – PersonEntity:
          Name:
            NameFull: Chen, Qi
      – PersonEntity:
          Name:
            NameFull: Lin, Yang
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 19
              M: 02
              Text: 2/19/2024
              Type: published
              Y: 2024
          Identifiers:
            – Type: issn-print
              Value: 01757598
          Numbering:
            – Type: volume
              Value: 108
            – Type: issue
              Value: 1
          Titles:
            – TitleFull: Applied Microbiology & Biotechnology
              Type: main
ResultId 1