View in EDS HTML Full Text PDF Full Text

X‐ray phase‐contrast tomography of cells manipulated with an optical stretcher.

Saved in:
Bibliographic Details
Title: X‐ray phase‐contrast tomography of cells manipulated with an optical stretcher.
Authors: Burchert, Jan-Philipp1,2 (AUTHOR), Frohn, Jasper1 (AUTHOR), Rölleke, Ulrike1 (AUTHOR), Bruns, Hendrik1 (AUTHOR), Yu, Boram1 (AUTHOR), Gleber, Sophie-Charlotte1 (AUTHOR), Stange, Roland3 (AUTHOR), Busse, Madleen4,5 (AUTHOR), Osterhoff, Markus1 (AUTHOR), Salditt, Tim1,2 (AUTHOR), Köster, Sarah1,2 (AUTHOR) sarah.koester@uni-goettingen.de
Source: Journal of Synchrotron Radiation. Jul2024, Vol. 31 Issue 4, p923-935. 13p.
Subjects: X-rays, Electron density, Tomography, X-ray imaging, Holography, Graphical projection, Electron holography
Abstract: X‐rays can penetrate deeply into biological cells and thus allow for examination of their internal structures with high spatial resolution. In this study, X‐ray phase‐contrast imaging and tomography is combined with an X‐ray‐compatible optical stretcher and microfluidic sample delivery. Using this setup, individual cells can be kept in suspension while they are examined with the X‐ray beam at a synchrotron. From the recorded holograms, 2D phase shift images that are proportional to the projected local electron density of the investigated cell can be calculated. From the tomographic reconstruction of multiple such projections the 3D electron density can be obtained. The cells can thus be studied in a hydrated or even living state, thus avoiding artifacts from freezing, drying or embedding, and can in principle also be subjected to different sample environments or mechanical strains. This combination of techniques is applied to living as well as fixed and stained NIH3T3 mouse fibroblasts and the effect of the beam energy on the phase shifts is investigated. Furthermore, a 3D algebraic reconstruction scheme and a dedicated mathematical description is used to follow the motion of the trapped cells in the optical stretcher for multiple rotations. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Synchrotron Radiation is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Full text is not displayed to guests.
FullText Links:
  – Type: pdflink
Text:
  Availability: 1
Header DbId: egs
DbLabel: Engineering Source
An: 178297676
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: X‐ray phase‐contrast tomography of cells manipulated with an optical stretcher.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Burchert%2C+Jan-Philipp%22">Burchert, Jan-Philipp</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Frohn%2C+Jasper%22">Frohn, Jasper</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Rölleke%2C+Ulrike%22">Rölleke, Ulrike</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bruns%2C+Hendrik%22">Bruns, Hendrik</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yu%2C+Boram%22">Yu, Boram</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Gleber%2C+Sophie-Charlotte%22">Gleber, Sophie-Charlotte</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Stange%2C+Roland%22">Stange, Roland</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Busse%2C+Madleen%22">Busse, Madleen</searchLink><relatesTo>4,5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Osterhoff%2C+Markus%22">Osterhoff, Markus</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Salditt%2C+Tim%22">Salditt, Tim</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Köster%2C+Sarah%22">Köster, Sarah</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> sarah.koester@uni-goettingen.de</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Journal+of+Synchrotron+Radiation%22">Journal of Synchrotron Radiation</searchLink>. Jul2024, Vol. 31 Issue 4, p923-935. 13p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22X-rays%22">X-rays</searchLink><br /><searchLink fieldCode="DE" term="%22Electron+density%22">Electron density</searchLink><br /><searchLink fieldCode="DE" term="%22Tomography%22">Tomography</searchLink><br /><searchLink fieldCode="DE" term="%22X-ray+imaging%22">X-ray imaging</searchLink><br /><searchLink fieldCode="DE" term="%22Holography%22">Holography</searchLink><br /><searchLink fieldCode="DE" term="%22Graphical+projection%22">Graphical projection</searchLink><br /><searchLink fieldCode="DE" term="%22Electron+holography%22">Electron holography</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: X‐rays can penetrate deeply into biological cells and thus allow for examination of their internal structures with high spatial resolution. In this study, X‐ray phase‐contrast imaging and tomography is combined with an X‐ray‐compatible optical stretcher and microfluidic sample delivery. Using this setup, individual cells can be kept in suspension while they are examined with the X‐ray beam at a synchrotron. From the recorded holograms, 2D phase shift images that are proportional to the projected local electron density of the investigated cell can be calculated. From the tomographic reconstruction of multiple such projections the 3D electron density can be obtained. The cells can thus be studied in a hydrated or even living state, thus avoiding artifacts from freezing, drying or embedding, and can in principle also be subjected to different sample environments or mechanical strains. This combination of techniques is applied to living as well as fixed and stained NIH3T3 mouse fibroblasts and the effect of the beam energy on the phase shifts is investigated. Furthermore, a 3D algebraic reconstruction scheme and a dedicated mathematical description is used to follow the motion of the trapped cells in the optical stretcher for multiple rotations. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Synchrotron Radiation is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=178297676
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1107/S1600577524003618
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 13
        StartPage: 923
    Subjects:
      – SubjectFull: X-rays
        Type: general
      – SubjectFull: Electron density
        Type: general
      – SubjectFull: Tomography
        Type: general
      – SubjectFull: X-ray imaging
        Type: general
      – SubjectFull: Holography
        Type: general
      – SubjectFull: Graphical projection
        Type: general
      – SubjectFull: Electron holography
        Type: general
    Titles:
      – TitleFull: X‐ray phase‐contrast tomography of cells manipulated with an optical stretcher.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Burchert, Jan-Philipp
      – PersonEntity:
          Name:
            NameFull: Frohn, Jasper
      – PersonEntity:
          Name:
            NameFull: Rölleke, Ulrike
      – PersonEntity:
          Name:
            NameFull: Bruns, Hendrik
      – PersonEntity:
          Name:
            NameFull: Yu, Boram
      – PersonEntity:
          Name:
            NameFull: Gleber, Sophie-Charlotte
      – PersonEntity:
          Name:
            NameFull: Stange, Roland
      – PersonEntity:
          Name:
            NameFull: Busse, Madleen
      – PersonEntity:
          Name:
            NameFull: Osterhoff, Markus
      – PersonEntity:
          Name:
            NameFull: Salditt, Tim
      – PersonEntity:
          Name:
            NameFull: Köster, Sarah
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 07
              Text: Jul2024
              Type: published
              Y: 2024
          Identifiers:
            – Type: issn-print
              Value: 09090495
          Numbering:
            – Type: volume
              Value: 31
            – Type: issue
              Value: 4
          Titles:
            – TitleFull: Journal of Synchrotron Radiation
              Type: main
ResultId 1