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Identification of stripe rust resistance gene YrBDT in Chinese landrace wheat Baidatou using BSE-seq and BSR-seq.

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Title: Identification of stripe rust resistance gene YrBDT in Chinese landrace wheat Baidatou using BSE-seq and BSR-seq.
Authors: Wu, Jingchun1,2 (AUTHOR), Cheng, Yukun3 (AUTHOR), Hao, Weihao4 (AUTHOR), Bai, Bin5 (AUTHOR), Fu, Luping6 (AUTHOR), Ren, Yan7 (AUTHOR), Hao, Yuanfeng2 (AUTHOR), Wang, Fengju2 (AUTHOR), Lin, Ruiming8 (AUTHOR), Si, Hongqi4 (AUTHOR), Ma, Chuanxi4 (AUTHOR), He, Zhonghu2,9 (AUTHOR), Chen, Jiansheng1 (AUTHOR) jshch@sdau.edu.cn, Chen, Can4 (AUTHOR) chencan-l@163.com, Xia, Xianchun2,3 (AUTHOR) xiaxianchun@caas.cn
Source: Theoretical & Applied Genetics. Sep2024, Vol. 137 Issue 9, p1-9. 9p.
Subjects: Stripe rust, Puccinia striiformis, Gene expression, Dominance (Genetics), Gene mapping, Wheat diseases & pests, Rust diseases
Abstract: Key message: A new stripe rust resistance gene YrBDT in Chinese landrace wheat Baidatou was mapped to a 943.6-kb interval on chromosome arm 6DS and co-segregated with a marker CAPS3 developed from candidate gene TraesCS6D03G0027300. Stripe rust caused by Puccinia striiformis f. sp. tritici (Pst) is a devastating foliar disease of wheat. Chinese landrace wheat Baidatou has shown high resistance to a broad spectrum of Pst races at both the seedling and adult-plant stages for decades in the Longnan region of Gansu province, a hot spot for stripe rust epidemics. Here, we report fine mapping and candidate gene analysis of stripe rust resistance gene YrBDT in Baidatou. Analysis of F1, F2 plants and F2:3 lines indicated that resistance in Baidatou to Pst race CYR31 was conferred by a single dominant gene, temporarily designated YrBDT. Bulked segregant exome capture sequencing (BSE-seq) analysis revealed 61 high-confidence polymorphic SNPs concentrated in a 5.4-Mb interval at the distal of chromosome arm 6DS. Several SNPs and InDels were also identified by genome mining of DNA sampled from the parents and contrasting bulks. The YrBDT locus was mapped to a 943.6-kb (4,658,322–5,601,880 bp) genomic region spanned by markers STS2 and STS3 based on IWGSC RefSeq v2.1, including five putative disease resistance genes. There was high collinearity of the target interval among Chinese Spring RefSeq v2.1, Ae. tauschii AL8/78 and Fielder genomes. The expression level of TraesCS6D03G0027300 showed significant association with Pst infection, and a gene-specific marker CAPS3 developed from TraesCS6D03G0027300 co-segregated with YrBDT suggesting this gene as a candidate of YrBDT. The resistance gene and flanking markers can be used in marker-assisted selection for improvement of stripe rust resistance. [ABSTRACT FROM AUTHOR]
Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Label: Title
  Group: Ti
  Data: Identification of stripe rust resistance gene YrBDT in Chinese landrace wheat Baidatou using BSE-seq and BSR-seq.
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  Label: Authors
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  Data: <searchLink fieldCode="AR" term="%22Wu%2C+Jingchun%22">Wu, Jingchun</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Cheng%2C+Yukun%22">Cheng, Yukun</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hao%2C+Weihao%22">Hao, Weihao</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bai%2C+Bin%22">Bai, Bin</searchLink><relatesTo>5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Fu%2C+Luping%22">Fu, Luping</searchLink><relatesTo>6</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ren%2C+Yan%22">Ren, Yan</searchLink><relatesTo>7</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hao%2C+Yuanfeng%22">Hao, Yuanfeng</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Fengju%22">Wang, Fengju</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lin%2C+Ruiming%22">Lin, Ruiming</searchLink><relatesTo>8</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Si%2C+Hongqi%22">Si, Hongqi</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ma%2C+Chuanxi%22">Ma, Chuanxi</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22He%2C+Zhonghu%22">He, Zhonghu</searchLink><relatesTo>2,9</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chen%2C+Jiansheng%22">Chen, Jiansheng</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> jshch@sdau.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Chen%2C+Can%22">Chen, Can</searchLink><relatesTo>4</relatesTo> (AUTHOR)<i> chencan-l@163.com</i><br /><searchLink fieldCode="AR" term="%22Xia%2C+Xianchun%22">Xia, Xianchun</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)<i> xiaxianchun@caas.cn</i>
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  Data: <searchLink fieldCode="JN" term="%22Theoretical+%26+Applied+Genetics%22">Theoretical & Applied Genetics</searchLink>. Sep2024, Vol. 137 Issue 9, p1-9. 9p.
– Name: Subject
  Label: Subjects
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  Data: <searchLink fieldCode="DE" term="%22Stripe+rust%22">Stripe rust</searchLink><br /><searchLink fieldCode="DE" term="%22Puccinia+striiformis%22">Puccinia striiformis</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Dominance+%28Genetics%29%22">Dominance (Genetics)</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+mapping%22">Gene mapping</searchLink><br /><searchLink fieldCode="DE" term="%22Wheat+diseases+%26+pests%22">Wheat diseases & pests</searchLink><br /><searchLink fieldCode="DE" term="%22Rust+diseases%22">Rust diseases</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Key message: A new stripe rust resistance gene YrBDT in Chinese landrace wheat Baidatou was mapped to a 943.6-kb interval on chromosome arm 6DS and co-segregated with a marker CAPS3 developed from candidate gene TraesCS6D03G0027300. Stripe rust caused by Puccinia striiformis f. sp. tritici (Pst) is a devastating foliar disease of wheat. Chinese landrace wheat Baidatou has shown high resistance to a broad spectrum of Pst races at both the seedling and adult-plant stages for decades in the Longnan region of Gansu province, a hot spot for stripe rust epidemics. Here, we report fine mapping and candidate gene analysis of stripe rust resistance gene YrBDT in Baidatou. Analysis of F1, F2 plants and F2:3 lines indicated that resistance in Baidatou to Pst race CYR31 was conferred by a single dominant gene, temporarily designated YrBDT. Bulked segregant exome capture sequencing (BSE-seq) analysis revealed 61 high-confidence polymorphic SNPs concentrated in a 5.4-Mb interval at the distal of chromosome arm 6DS. Several SNPs and InDels were also identified by genome mining of DNA sampled from the parents and contrasting bulks. The YrBDT locus was mapped to a 943.6-kb (4,658,322–5,601,880 bp) genomic region spanned by markers STS2 and STS3 based on IWGSC RefSeq v2.1, including five putative disease resistance genes. There was high collinearity of the target interval among Chinese Spring RefSeq v2.1, Ae. tauschii AL8/78 and Fielder genomes. The expression level of TraesCS6D03G0027300 showed significant association with Pst infection, and a gene-specific marker CAPS3 developed from TraesCS6D03G0027300 co-segregated with YrBDT suggesting this gene as a candidate of YrBDT. The resistance gene and flanking markers can be used in marker-assisted selection for improvement of stripe rust resistance. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1007/s00122-024-04704-y
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        Text: English
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      – SubjectFull: Stripe rust
        Type: general
      – SubjectFull: Puccinia striiformis
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      – SubjectFull: Gene expression
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      – SubjectFull: Dominance (Genetics)
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      – SubjectFull: Gene mapping
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      – SubjectFull: Wheat diseases & pests
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      – SubjectFull: Rust diseases
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      – TitleFull: Identification of stripe rust resistance gene YrBDT in Chinese landrace wheat Baidatou using BSE-seq and BSR-seq.
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