Separation of weak acids, neutral compounds, and permanent anions using sequential elution liquid chromatography with tandem columns.
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| Title: | Separation of weak acids, neutral compounds, and permanent anions using sequential elution liquid chromatography with tandem columns. |
|---|---|
| Authors: | Lovejoy, Lauren Kline1,2 (AUTHOR), Foley, Joe P.1,2 (AUTHOR) jfoley@drexel.edu |
| Source: | Journal of Chromatography A. Aug2024, Vol. 1731, pN.PAG-N.PAG. 1p. |
| Subjects: | Liquid chromatography, Column chromatography, RF values (Chromatography), Chromatographic analysis, High performance liquid chromatography |
| Abstract: | • An alternative elution mode to conventional HPLC to separate compounds by class. • Sequential elution was utilized to separate classes of compounds in under 15 min. • A tandem-column stationary phase combination (SAX and C18) was utilized. • SAX+C18 and C18+SAX column orientations yielded nearly identical separations. • Retention time and peak area precision were below 1.5 % RSD. This paper discusses the development of an analytical method by an alternative separation approach, sequential elution liquid chromatography (SE-LC), to separate permanently charged ions (anions), weak acids, and neutral compounds using anion exchange and reversed-phase columns in tandem. SE-LC separates classes of compounds by group by employing two or more elution modes. Advantages to using SE-LC over conventional HPLC are a greater peak capacity and a reduced separation disorder. Importantly, the same HPLC as used for a conventional HPLC separation may be used to afford a successful SE-LC separation. Mobile phase selection and gradient optimization are integral for a successful SE-LC class separation of permanent anions, weak acids, and neutral compounds and will be discussed in detail in this paper. The most successful (best resolution and repeatability) SE-LC separation was achieved by applying isocratic elution at low pH to elute the weak acids, followed by an acetonitrile gradient to elute the neutral compounds, and last a sodium methanesulfonate gradient to elute the anionic compounds using a superficially porous C18 column coupled with a strong anion exchange (SAX) column. Repeatability (RSD) in the retention times and peak areas of the analytes was less than 0.25 % and 1.5 %, respectively. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 178942827 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Separation of weak acids, neutral compounds, and permanent anions using sequential elution liquid chromatography with tandem columns. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Lovejoy%2C+Lauren+Kline%22">Lovejoy, Lauren Kline</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Foley%2C+Joe+P%2E%22">Foley, Joe P.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> jfoley@drexel.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Chromatography+A%22">Journal of Chromatography A</searchLink>. Aug2024, Vol. 1731, pN.PAG-N.PAG. 1p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Liquid+chromatography%22">Liquid chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Column+chromatography%22">Column chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22RF+values+%28Chromatography%29%22">RF values (Chromatography)</searchLink><br /><searchLink fieldCode="DE" term="%22Chromatographic+analysis%22">Chromatographic analysis</searchLink><br /><searchLink fieldCode="DE" term="%22High+performance+liquid+chromatography%22">High performance liquid chromatography</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: • An alternative elution mode to conventional HPLC to separate compounds by class. • Sequential elution was utilized to separate classes of compounds in under 15 min. • A tandem-column stationary phase combination (SAX and C18) was utilized. • SAX+C18 and C18+SAX column orientations yielded nearly identical separations. • Retention time and peak area precision were below 1.5 % RSD. This paper discusses the development of an analytical method by an alternative separation approach, sequential elution liquid chromatography (SE-LC), to separate permanently charged ions (anions), weak acids, and neutral compounds using anion exchange and reversed-phase columns in tandem. SE-LC separates classes of compounds by group by employing two or more elution modes. Advantages to using SE-LC over conventional HPLC are a greater peak capacity and a reduced separation disorder. Importantly, the same HPLC as used for a conventional HPLC separation may be used to afford a successful SE-LC separation. Mobile phase selection and gradient optimization are integral for a successful SE-LC class separation of permanent anions, weak acids, and neutral compounds and will be discussed in detail in this paper. The most successful (best resolution and repeatability) SE-LC separation was achieved by applying isocratic elution at low pH to elute the weak acids, followed by an acetonitrile gradient to elute the neutral compounds, and last a sodium methanesulfonate gradient to elute the anionic compounds using a superficially porous C18 column coupled with a strong anion exchange (SAX) column. Repeatability (RSD) in the retention times and peak areas of the analytes was less than 0.25 % and 1.5 %, respectively. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.chroma.2024.465178 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 1 StartPage: N.PAG Subjects: – SubjectFull: Liquid chromatography Type: general – SubjectFull: Column chromatography Type: general – SubjectFull: RF values (Chromatography) Type: general – SubjectFull: Chromatographic analysis Type: general – SubjectFull: High performance liquid chromatography Type: general Titles: – TitleFull: Separation of weak acids, neutral compounds, and permanent anions using sequential elution liquid chromatography with tandem columns. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Lovejoy, Lauren Kline – PersonEntity: Name: NameFull: Foley, Joe P. IsPartOfRelationships: – BibEntity: Dates: – D: 30 M: 08 Text: Aug2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 00219673 Numbering: – Type: volume Value: 1731 Titles: – TitleFull: Journal of Chromatography A Type: main |
| ResultId | 1 |
