Continuous flow delivery system for the perfusion of scaffold-based 3D cultures.
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| Title: | Continuous flow delivery system for the perfusion of scaffold-based 3D cultures. |
|---|---|
| Authors: | Sitte, Zachary R.1, Karlsson, Elizabeth E.1, Li, Haolin2, Zhou, Haibo2,3, Lockett, Matthew R.1,4 mlockett@unc.edu |
| Source: | Lab on a Chip. 9/7/2024, Vol. 24 Issue 17, p4105-4114. 10p. |
| Subjects: | Perfusion, Microfluidic devices, Three-dimensional printing, Endothelial cells, Material culture, Cell separation |
| Abstract: | The paper-based culture platform developed by Whitesides readily incorporates tissue-like structures into laboratories with established workflows that rely on monolayer cultures. Cell-laden hydrogels are deposited in these porous scaffolds with micropipettes; these scaffolds support the thin gel slabs, allowing them to be evaluated individually or stacked into thick constructs. The paper-based culture platform has inspired many basic and translational studies, each exploring how readily accessible materials can generate complex structures that mimic aspects of tissues in vivo. Many of these examples have relied on static culture conditions, which result in diffusion-limited environments and cells experiencing pericellular hypoxia. Perfusion-based systems can alleviate pericellular hypoxia and other cell stresses by continually exposing the cells to fresh medium. These perfusion systems are common in microfluidic and organ-on-chip devices supporting cells as monolayer cultures or as 3D constructs. Here, we introduce a continuous flow delivery system, which uses parts readily produced with 3D printing to provide a self-contained culture platform in which cells in paper or other scaffolds are exposed to fresh (flowing) medium. We demonstrate the utility of this device with examples of cells maintained in single cell-laden scaffolds, stacks of cell-laden scaffolds, and scaffolds that contain monolayers of endothelial cells. These demonstrations highlight some possible experimental questions that can be enabled with readily accessible culture materials and a perfusion-based device that can be readily fabricated. [ABSTRACT FROM AUTHOR] |
| Copyright of Lab on a Chip is the property of Royal Society of Chemistry and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 179111513 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Continuous flow delivery system for the perfusion of scaffold-based 3D cultures. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Sitte%2C+Zachary+R%2E%22">Sitte, Zachary R.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Karlsson%2C+Elizabeth+E%2E%22">Karlsson, Elizabeth E.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Li%2C+Haolin%22">Li, Haolin</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhou%2C+Haibo%22">Zhou, Haibo</searchLink><relatesTo>2,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Lockett%2C+Matthew+R%2E%22">Lockett, Matthew R.</searchLink><relatesTo>1,4</relatesTo><i> mlockett@unc.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Lab+on+a+Chip%22">Lab on a Chip</searchLink>. 9/7/2024, Vol. 24 Issue 17, p4105-4114. 10p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Perfusion%22">Perfusion</searchLink><br /><searchLink fieldCode="DE" term="%22Microfluidic+devices%22">Microfluidic devices</searchLink><br /><searchLink fieldCode="DE" term="%22Three-dimensional+printing%22">Three-dimensional printing</searchLink><br /><searchLink fieldCode="DE" term="%22Endothelial+cells%22">Endothelial cells</searchLink><br /><searchLink fieldCode="DE" term="%22Material+culture%22">Material culture</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+separation%22">Cell separation</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The paper-based culture platform developed by Whitesides readily incorporates tissue-like structures into laboratories with established workflows that rely on monolayer cultures. Cell-laden hydrogels are deposited in these porous scaffolds with micropipettes; these scaffolds support the thin gel slabs, allowing them to be evaluated individually or stacked into thick constructs. The paper-based culture platform has inspired many basic and translational studies, each exploring how readily accessible materials can generate complex structures that mimic aspects of tissues in vivo. Many of these examples have relied on static culture conditions, which result in diffusion-limited environments and cells experiencing pericellular hypoxia. Perfusion-based systems can alleviate pericellular hypoxia and other cell stresses by continually exposing the cells to fresh medium. These perfusion systems are common in microfluidic and organ-on-chip devices supporting cells as monolayer cultures or as 3D constructs. Here, we introduce a continuous flow delivery system, which uses parts readily produced with 3D printing to provide a self-contained culture platform in which cells in paper or other scaffolds are exposed to fresh (flowing) medium. We demonstrate the utility of this device with examples of cells maintained in single cell-laden scaffolds, stacks of cell-laden scaffolds, and scaffolds that contain monolayers of endothelial cells. These demonstrations highlight some possible experimental questions that can be enabled with readily accessible culture materials and a perfusion-based device that can be readily fabricated. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Lab on a Chip is the property of Royal Society of Chemistry and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=179111513 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1039/d4lc00480a Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 4105 Subjects: – SubjectFull: Perfusion Type: general – SubjectFull: Microfluidic devices Type: general – SubjectFull: Three-dimensional printing Type: general – SubjectFull: Endothelial cells Type: general – SubjectFull: Material culture Type: general – SubjectFull: Cell separation Type: general Titles: – TitleFull: Continuous flow delivery system for the perfusion of scaffold-based 3D cultures. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Sitte, Zachary R. – PersonEntity: Name: NameFull: Karlsson, Elizabeth E. – PersonEntity: Name: NameFull: Li, Haolin – PersonEntity: Name: NameFull: Zhou, Haibo – PersonEntity: Name: NameFull: Lockett, Matthew R. IsPartOfRelationships: – BibEntity: Dates: – D: 07 M: 09 Text: 9/7/2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 14730197 Numbering: – Type: volume Value: 24 – Type: issue Value: 17 Titles: – TitleFull: Lab on a Chip Type: main |
| ResultId | 1 |
