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Validation of a simple bioanalytical method for the determination of melatonin concentration in human serum using high-performance liquid chromatography with a fluorescent detector.

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Title: Validation of a simple bioanalytical method for the determination of melatonin concentration in human serum using high-performance liquid chromatography with a fluorescent detector.
Authors: Lovrić, Mila1,2,3 (AUTHOR) mila.lovric@kbc-zagreb.hr, Jelić-Knezović, Nevenka4 (AUTHOR), Ščavničar, Andrijana1 (AUTHOR), Miloš, Marija1,5 (AUTHOR), Rogić, Dunja1,2 (AUTHOR)
Source: Journal of Liquid Chromatography & Related Technologies. 2024, Vol. 47 Issue 11-15, p201-207. 7p.
Subjects: High performance liquid chromatography, Chromatographic detectors, Ethyl acetate, Mirtazapine, Detection limit
Abstract: Analysis of melatonin in biological samples is a challenge for the analyst both because of its low concentration and because of the numerous interfering substances. Therefore, chromatography, as a powerful analytical technique used for the separation, detection, and quantification of analytes, can be the method of choice for determining melatonin in biological samples for clinical use and pharmacokinetic research. The method was developed and validated according to the internal documents in accordance with Good Laboratory Practice. The concentrations of melatonin in human serum were determined using a high-performance liquid chromatography method with a fluorescence detector and mirtazapine as an internal standard. Preparation of serum samples was performed by liquid–liquid extraction with ethyl acetate: n-hexane (1:1 v/v). High linearity was exhibited over a concentration range of 25–10,000 pg/mL of melatonin, with a correlation coefficient higher than 0.999 during the course of method validation. Repeatability and intermediate precision show a relative standard deviation lower than 6.6 %, and accuracies were from 90.7 % to 110.3 %. The lower limit of quantitation was 30 pg/mL, and the limit of detection was 7.92 pg/mL. The in-house method is simple, fast, reproducible, and applicable for the determination of melatonin in human serum. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Liquid Chromatography & Related Technologies is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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An: 179170190
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  Label: Title
  Group: Ti
  Data: Validation of a simple bioanalytical method for the determination of melatonin concentration in human serum using high-performance liquid chromatography with a fluorescent detector.
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  Data: <searchLink fieldCode="AR" term="%22Lovrić%2C+Mila%22">Lovrić, Mila</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<i> mila.lovric@kbc-zagreb.hr</i><br /><searchLink fieldCode="AR" term="%22Jelić-Knezović%2C+Nevenka%22">Jelić-Knezović, Nevenka</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ščavničar%2C+Andrijana%22">Ščavničar, Andrijana</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Miloš%2C+Marija%22">Miloš, Marija</searchLink><relatesTo>1,5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Rogić%2C+Dunja%22">Rogić, Dunja</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Liquid+Chromatography+%26+Related+Technologies%22">Journal of Liquid Chromatography & Related Technologies</searchLink>. 2024, Vol. 47 Issue 11-15, p201-207. 7p.
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  Data: <searchLink fieldCode="DE" term="%22High+performance+liquid+chromatography%22">High performance liquid chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Chromatographic+detectors%22">Chromatographic detectors</searchLink><br /><searchLink fieldCode="DE" term="%22Ethyl+acetate%22">Ethyl acetate</searchLink><br /><searchLink fieldCode="DE" term="%22Mirtazapine%22">Mirtazapine</searchLink><br /><searchLink fieldCode="DE" term="%22Detection+limit%22">Detection limit</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Analysis of melatonin in biological samples is a challenge for the analyst both because of its low concentration and because of the numerous interfering substances. Therefore, chromatography, as a powerful analytical technique used for the separation, detection, and quantification of analytes, can be the method of choice for determining melatonin in biological samples for clinical use and pharmacokinetic research. The method was developed and validated according to the internal documents in accordance with Good Laboratory Practice. The concentrations of melatonin in human serum were determined using a high-performance liquid chromatography method with a fluorescence detector and mirtazapine as an internal standard. Preparation of serum samples was performed by liquid–liquid extraction with ethyl acetate: n-hexane (1:1 v/v). High linearity was exhibited over a concentration range of 25–10,000 pg/mL of melatonin, with a correlation coefficient higher than 0.999 during the course of method validation. Repeatability and intermediate precision show a relative standard deviation lower than 6.6 %, and accuracies were from 90.7 % to 110.3 %. The lower limit of quantitation was 30 pg/mL, and the limit of detection was 7.92 pg/mL. The in-house method is simple, fast, reproducible, and applicable for the determination of melatonin in human serum. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Liquid Chromatography & Related Technologies is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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        Value: 10.1080/10826076.2024.2348167
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        Text: English
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        PageCount: 7
        StartPage: 201
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        Type: general
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      – SubjectFull: Ethyl acetate
        Type: general
      – SubjectFull: Mirtazapine
        Type: general
      – SubjectFull: Detection limit
        Type: general
    Titles:
      – TitleFull: Validation of a simple bioanalytical method for the determination of melatonin concentration in human serum using high-performance liquid chromatography with a fluorescent detector.
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            NameFull: Lovrić, Mila
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            NameFull: Jelić-Knezović, Nevenka
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            NameFull: Ščavničar, Andrijana
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            NameFull: Miloš, Marija
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            NameFull: Rogić, Dunja
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            – D: 01
              M: 06
              Text: 2024
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