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Viral and thermal lysis facilitates transmission of antibiotic resistance genes during composting.

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Title: Viral and thermal lysis facilitates transmission of antibiotic resistance genes during composting.
Authors: Chaofan Ai1, Peng Cui2, Chen Liu1, Jiawei Wu1, Yuan Xu1, Xiaolong Liang3, Qiu-e. Yang1, Xiang Tang1, Shungui Zhou1, Hanpeng Liao1 liaohp@fafu.edu.cn, Friman, Ville-Petri4
Source: Applied & Environmental Microbiology. Aug2024, Vol. 90 Issue 8, p1-20. 20p.
Subjects: Cattle manure, Drug resistance in bacteria, Composting, Viral DNA, Multiomics
Abstract: While the distribution of extracellular ARGs (eARGs) in the environment has been widely reported, the factors governing their release remain poorly understood. Here, we combined multi-omics and direct experimentation to test whether the release and transmission of eARGs are associated with viral lysis and heat during cow manure composting. Our results reveal that the proportion of eARGs increased 2.7-fold during composting, despite a significant and concomitant reduction in intracellular ARG abundances. This relative increase of eARGs was driven by composting temperature and viral lysis of ARG-carrying bacteria based on metagenome-assembled genome (MAG) analysis. Notably, thermal lysis of mesophilic bacteria carrying ARGs was a key factor in releasing eARGs at the thermophilic phase, while viral lysis played a relatively stronger role during the non-thermal phase of composting. Furthermore, MAG-based tracking of ARGs in combination with direct transformation experiments demonstrated that eARGs released during composting pose a potential transmission risk. Our study provides bioinformatic and experimental evidence of the undiscovered role of temperature and viral lysis in co-driving the spread of ARGs in compost microbiomes via the horizontal transfer of environmentally released DNA. [ABSTRACT FROM AUTHOR]
Copyright of Applied & Environmental Microbiology is the property of American Society for Microbiology and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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Header DbId: egs
DbLabel: Engineering Source
An: 179446306
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Viral and thermal lysis facilitates transmission of antibiotic resistance genes during composting.
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  Data: <searchLink fieldCode="AR" term="%22Chaofan+Ai%22">Chaofan Ai</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Peng+Cui%22">Peng Cui</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Chen+Liu%22">Chen Liu</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Jiawei+Wu%22">Jiawei Wu</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yuan+Xu%22">Yuan Xu</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Xiaolong+Liang%22">Xiaolong Liang</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Qiu-e%2E+Yang%22">Qiu-e. Yang</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Xiang+Tang%22">Xiang Tang</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Shungui+Zhou%22">Shungui Zhou</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Hanpeng+Liao%22">Hanpeng Liao</searchLink><relatesTo>1</relatesTo><i> liaohp@fafu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Friman%2C+Ville-Petri%22">Friman, Ville-Petri</searchLink><relatesTo>4</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Applied+%26+Environmental+Microbiology%22">Applied & Environmental Microbiology</searchLink>. Aug2024, Vol. 90 Issue 8, p1-20. 20p.
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  Data: <searchLink fieldCode="DE" term="%22Cattle+manure%22">Cattle manure</searchLink><br /><searchLink fieldCode="DE" term="%22Drug+resistance+in+bacteria%22">Drug resistance in bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22Composting%22">Composting</searchLink><br /><searchLink fieldCode="DE" term="%22Viral+DNA%22">Viral DNA</searchLink><br /><searchLink fieldCode="DE" term="%22Multiomics%22">Multiomics</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: While the distribution of extracellular ARGs (eARGs) in the environment has been widely reported, the factors governing their release remain poorly understood. Here, we combined multi-omics and direct experimentation to test whether the release and transmission of eARGs are associated with viral lysis and heat during cow manure composting. Our results reveal that the proportion of eARGs increased 2.7-fold during composting, despite a significant and concomitant reduction in intracellular ARG abundances. This relative increase of eARGs was driven by composting temperature and viral lysis of ARG-carrying bacteria based on metagenome-assembled genome (MAG) analysis. Notably, thermal lysis of mesophilic bacteria carrying ARGs was a key factor in releasing eARGs at the thermophilic phase, while viral lysis played a relatively stronger role during the non-thermal phase of composting. Furthermore, MAG-based tracking of ARGs in combination with direct transformation experiments demonstrated that eARGs released during composting pose a potential transmission risk. Our study provides bioinformatic and experimental evidence of the undiscovered role of temperature and viral lysis in co-driving the spread of ARGs in compost microbiomes via the horizontal transfer of environmentally released DNA. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Applied & Environmental Microbiology is the property of American Society for Microbiology and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1128/aem.00695-24
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        Text: English
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        PageCount: 20
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      – SubjectFull: Cattle manure
        Type: general
      – SubjectFull: Drug resistance in bacteria
        Type: general
      – SubjectFull: Composting
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      – SubjectFull: Viral DNA
        Type: general
      – SubjectFull: Multiomics
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      – TitleFull: Viral and thermal lysis facilitates transmission of antibiotic resistance genes during composting.
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              M: 08
              Text: Aug2024
              Type: published
              Y: 2024
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