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Femtosecond laser-assisted selective holding with ultra-low power for direct manipulation of biological species.

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Title: Femtosecond laser-assisted selective holding with ultra-low power for direct manipulation of biological species.
Authors: Krishna, Krishangi1 (AUTHOR) krishangi_krishna@brown.edu, Burrow, Joshua A.1 (AUTHOR) joshua_burrow@brown.edu, Jiang, Zhaowei2 (AUTHOR) zhaowei_jiang@brown.edu, Liu, Wenyu1 (AUTHOR) wenyu_liu@brown.edu, Shukla, Anita2 (AUTHOR) anita_shukla@brown.edu, Toussaint Jr., Kimani C.1,3 (AUTHOR) kimani_toussaint@brown.edu
Source: Journal of Biomedical Optics. Aug2024, Vol. 29 Issue 8, p86501-86501. 1p.
Subjects: Continuous wave lasers, Femtosecond lasers, Biological specimens, Bacteria morphology, Cell motility, Optical tweezers
Abstract: Optical tweezers (OTs) have emerged as an essential technique for manipulating nanoscopic particulates and biological specimens with sub-micron precision and have revolutionized various fields, including biology and colloidal physics. However, traditional optical trapping techniques often rely on moderate- to high-power continuous wave (CW) lasers, which can introduce unwanted thermal effects and photodamage to delicate samples. An innovative alternative has emerged through the utilization of femtosecond (fs) lasers at ultra-low average powers on the order of tens of microwatt. Unexpectedly overlooked until now, this method enables the direct trapping and manipulation of cells without relying on functionalized spheres. We aim to compare the trap stiffness of CW and fs lasers in an unexplored average power regime (sub-1 mW) on cells within the intermediate-size regime. A CW or fs laser is used to trap cells in an inverted microscope setup. We trap five different pathogenic bacteria with different morphologies to compare trap stiffness. We find that fs laser-assisted selective holding with ultra-low power (FLASH-UP) exhibits five times greater trap stiffness than CW-based OTs and can trap at lower intensities. Furthermore, we demonstrate that FLASH-UP does not impact cell motility. FLASH-UP displays higher trap stiffness at average powers below 1 mW and does not impact cell functionality. These results pave the way for ultra-low-power trapping of cells for applications in sorting, bio-sensing, in vivo cell manipulation, and single-cell analysis. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Biomedical Optics is the property of SPIE - International Society of Optical Engineering and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 179736058
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Data: Femtosecond laser-assisted selective holding with ultra-low power for direct manipulation of biological species.
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  Data: <searchLink fieldCode="AR" term="%22Krishna%2C+Krishangi%22">Krishna, Krishangi</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> krishangi_krishna@brown.edu</i><br /><searchLink fieldCode="AR" term="%22Burrow%2C+Joshua+A%2E%22">Burrow, Joshua A.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> joshua_burrow@brown.edu</i><br /><searchLink fieldCode="AR" term="%22Jiang%2C+Zhaowei%22">Jiang, Zhaowei</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> zhaowei_jiang@brown.edu</i><br /><searchLink fieldCode="AR" term="%22Liu%2C+Wenyu%22">Liu, Wenyu</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> wenyu_liu@brown.edu</i><br /><searchLink fieldCode="AR" term="%22Shukla%2C+Anita%22">Shukla, Anita</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> anita_shukla@brown.edu</i><br /><searchLink fieldCode="AR" term="%22Toussaint+Jr%2E%2C+Kimani+C%2E%22">Toussaint Jr., Kimani C.</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)<i> kimani_toussaint@brown.edu</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Biomedical+Optics%22">Journal of Biomedical Optics</searchLink>. Aug2024, Vol. 29 Issue 8, p86501-86501. 1p.
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  Data: <searchLink fieldCode="DE" term="%22Continuous+wave+lasers%22">Continuous wave lasers</searchLink><br /><searchLink fieldCode="DE" term="%22Femtosecond+lasers%22">Femtosecond lasers</searchLink><br /><searchLink fieldCode="DE" term="%22Biological+specimens%22">Biological specimens</searchLink><br /><searchLink fieldCode="DE" term="%22Bacteria+morphology%22">Bacteria morphology</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+motility%22">Cell motility</searchLink><br /><searchLink fieldCode="DE" term="%22Optical+tweezers%22">Optical tweezers</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Optical tweezers (OTs) have emerged as an essential technique for manipulating nanoscopic particulates and biological specimens with sub-micron precision and have revolutionized various fields, including biology and colloidal physics. However, traditional optical trapping techniques often rely on moderate- to high-power continuous wave (CW) lasers, which can introduce unwanted thermal effects and photodamage to delicate samples. An innovative alternative has emerged through the utilization of femtosecond (fs) lasers at ultra-low average powers on the order of tens of microwatt. Unexpectedly overlooked until now, this method enables the direct trapping and manipulation of cells without relying on functionalized spheres. We aim to compare the trap stiffness of CW and fs lasers in an unexplored average power regime (sub-1 mW) on cells within the intermediate-size regime. A CW or fs laser is used to trap cells in an inverted microscope setup. We trap five different pathogenic bacteria with different morphologies to compare trap stiffness. We find that fs laser-assisted selective holding with ultra-low power (FLASH-UP) exhibits five times greater trap stiffness than CW-based OTs and can trap at lower intensities. Furthermore, we demonstrate that FLASH-UP does not impact cell motility. FLASH-UP displays higher trap stiffness at average powers below 1 mW and does not impact cell functionality. These results pave the way for ultra-low-power trapping of cells for applications in sorting, bio-sensing, in vivo cell manipulation, and single-cell analysis. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Biomedical Optics is the property of SPIE - International Society of Optical Engineering and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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        Value: 10.1117/1.JBO.29.8.086501
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      – Code: eng
        Text: English
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        PageCount: 1
        StartPage: 86501
    Subjects:
      – SubjectFull: Continuous wave lasers
        Type: general
      – SubjectFull: Femtosecond lasers
        Type: general
      – SubjectFull: Biological specimens
        Type: general
      – SubjectFull: Bacteria morphology
        Type: general
      – SubjectFull: Cell motility
        Type: general
      – SubjectFull: Optical tweezers
        Type: general
    Titles:
      – TitleFull: Femtosecond laser-assisted selective holding with ultra-low power for direct manipulation of biological species.
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            NameFull: Krishna, Krishangi
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            NameFull: Burrow, Joshua A.
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            NameFull: Jiang, Zhaowei
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            NameFull: Liu, Wenyu
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            NameFull: Shukla, Anita
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            – D: 01
              M: 08
              Text: Aug2024
              Type: published
              Y: 2024
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              Value: 29
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