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TaNAC1 boosts powdery mildew resistance by phosphorylation‐dependent regulation of TaSec1a and TaCAMTA4 via PP2Ac/CDPK20.

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Title: TaNAC1 boosts powdery mildew resistance by phosphorylation‐dependent regulation of TaSec1a and TaCAMTA4 via PP2Ac/CDPK20.
Authors: Liu, Yuanming1 (AUTHOR), You, Hongguang2 (AUTHOR), Li, Hanping1 (AUTHOR), Zhang, Chujun2 (AUTHOR), Guo, Huan1 (AUTHOR), Huang, Xueling3 (AUTHOR), Zhang, Qiong3 (AUTHOR), Zhang, Xiangyu1 (AUTHOR), Ma, Chuang2 (AUTHOR), Wang, Yajuan1 (AUTHOR), Li, Tingdong1 (AUTHOR), Ji, Wanquan1 (AUTHOR), Kang, Zhensheng3 (AUTHOR) kangzs@nwsuaf.edu.cn, Zhang, Hong1 (AUTHOR) zhangh1129@nwafu.edu.cn
Source: New Phytologist. Oct2024, Vol. 244 Issue 2, p635-653. 19p.
Subjects: Transcription factors, Nuclear proteins, Phosphoprotein phosphatases, Powdery mildew diseases, Nucleic acids
Abstract: Summary: The integrity of wheat (Triticum aestivum) production is increasingly jeopardized by the fungal pathogen Blumeria graminis f. sp. tritici (Bgt), particularly amid the vicissitudes of climate change.Here, we delineated the role of a wheat transcription factor, TaNAC1, which precipitates cellular apoptosis and fortifies resistance against Bgt. Utilizing BiFC, co‐immunoprecipitation, protein quantification, luciferase report assays, we determined that cytoplasmic TaNAC1‐7A undergoes phosphorylation at the S184/S258 sites by TaCDPK20, facilitating its nuclear translocation. This migration appears to prime further phosphorylation by TaMPK1, thereby enhancing transcriptional regulatory activity. Notably, the apoptotic activity of phosphorylated TaNAC1‐7A is negatively modulated by the nuclear protein phosphatase PP2Ac.Furthermore, activation of TaNAC1 phosphorylation initiates transcription of downstream genes TaSec1a and TaCAMTA4, through binding to the C[T/G]T[N7]A[A/C]G nucleic acid motif. Suppression of TaNAC1, TaCDPK20, and TaMPK1 in wheat compromises its resistance to Bgt strain E09, whereas overexpression of TaNAC1 and silencing of PP2Ac markedly elevate resistance levels.Our results reveal the pivotal role of TaNAC1 in basal resistance which is mediated by its effects on homotypic fusion, vacuolar protein sorting, and the expression of defense‐related genes. The findings highlight the potential through targeting TaNAC1 and its regulators as a strategy for improving wheat's resistance to fungal pathogens. [ABSTRACT FROM AUTHOR]
Copyright of New Phytologist is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: TaNAC1 boosts powdery mildew resistance by phosphorylation‐dependent regulation of TaSec1a and TaCAMTA4 via PP2Ac/CDPK20.
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  Data: <searchLink fieldCode="AR" term="%22Liu%2C+Yuanming%22">Liu, Yuanming</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22You%2C+Hongguang%22">You, Hongguang</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Li%2C+Hanping%22">Li, Hanping</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Chujun%22">Zhang, Chujun</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Guo%2C+Huan%22">Guo, Huan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Huang%2C+Xueling%22">Huang, Xueling</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Qiong%22">Zhang, Qiong</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Xiangyu%22">Zhang, Xiangyu</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ma%2C+Chuang%22">Ma, Chuang</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Yajuan%22">Wang, Yajuan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Li%2C+Tingdong%22">Li, Tingdong</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ji%2C+Wanquan%22">Ji, Wanquan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kang%2C+Zhensheng%22">Kang, Zhensheng</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> kangzs@nwsuaf.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Hong%22">Zhang, Hong</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> zhangh1129@nwafu.edu.cn</i>
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  Data: <searchLink fieldCode="JN" term="%22New+Phytologist%22">New Phytologist</searchLink>. Oct2024, Vol. 244 Issue 2, p635-653. 19p.
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  Data: <searchLink fieldCode="DE" term="%22Transcription+factors%22">Transcription factors</searchLink><br /><searchLink fieldCode="DE" term="%22Nuclear+proteins%22">Nuclear proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Phosphoprotein+phosphatases%22">Phosphoprotein phosphatases</searchLink><br /><searchLink fieldCode="DE" term="%22Powdery+mildew+diseases%22">Powdery mildew diseases</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleic+acids%22">Nucleic acids</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Summary: The integrity of wheat (Triticum aestivum) production is increasingly jeopardized by the fungal pathogen Blumeria graminis f. sp. tritici (Bgt), particularly amid the vicissitudes of climate change.Here, we delineated the role of a wheat transcription factor, TaNAC1, which precipitates cellular apoptosis and fortifies resistance against Bgt. Utilizing BiFC, co‐immunoprecipitation, protein quantification, luciferase report assays, we determined that cytoplasmic TaNAC1‐7A undergoes phosphorylation at the S184/S258 sites by TaCDPK20, facilitating its nuclear translocation. This migration appears to prime further phosphorylation by TaMPK1, thereby enhancing transcriptional regulatory activity. Notably, the apoptotic activity of phosphorylated TaNAC1‐7A is negatively modulated by the nuclear protein phosphatase PP2Ac.Furthermore, activation of TaNAC1 phosphorylation initiates transcription of downstream genes TaSec1a and TaCAMTA4, through binding to the C[T/G]T[N7]A[A/C]G nucleic acid motif. Suppression of TaNAC1, TaCDPK20, and TaMPK1 in wheat compromises its resistance to Bgt strain E09, whereas overexpression of TaNAC1 and silencing of PP2Ac markedly elevate resistance levels.Our results reveal the pivotal role of TaNAC1 in basal resistance which is mediated by its effects on homotypic fusion, vacuolar protein sorting, and the expression of defense‐related genes. The findings highlight the potential through targeting TaNAC1 and its regulators as a strategy for improving wheat's resistance to fungal pathogens. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
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  Data: <i>Copyright of New Phytologist is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1111/nph.20070
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      – Code: eng
        Text: English
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        PageCount: 19
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      – SubjectFull: Transcription factors
        Type: general
      – SubjectFull: Nuclear proteins
        Type: general
      – SubjectFull: Phosphoprotein phosphatases
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      – SubjectFull: Powdery mildew diseases
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      – SubjectFull: Nucleic acids
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      – TitleFull: TaNAC1 boosts powdery mildew resistance by phosphorylation‐dependent regulation of TaSec1a and TaCAMTA4 via PP2Ac/CDPK20.
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              Text: Oct2024
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