A single-nucleotide insertion in Rxp confers durable resistance to bacterial pustule in soybean.
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| Title: | A single-nucleotide insertion in Rxp confers durable resistance to bacterial pustule in soybean. |
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| Authors: | Taguchi-Shiobara, Fumio1,2 (AUTHOR) fstagu@naro.affrc.go.jp, Takahashi, Koji1 (AUTHOR), Yano, Ryoichi3 (AUTHOR), Suzuki, Rintaro3 (AUTHOR), Yokota, Yuko1 (AUTHOR), Yamazaki, Toshimasa3 (AUTHOR), Yamada, Tetsuya1,4 (AUTHOR), Sayama, Takashi1,5 (AUTHOR), Yamada, Naohiro1,6 (AUTHOR), Oki, Nobuhiko7 (AUTHOR), Anai, Toyoaki8,9 (AUTHOR), Kaga, Akito1 (AUTHOR), Ishimoto, Masao1 (AUTHOR) ishimoto@naro.affrc.go.jp |
| Source: | Theoretical & Applied Genetics. Nov2024, Vol. 137 Issue 11, p1-15. 15p. |
| Abstract: | Key message: The soybean Rxp gene, encoding a bHLH transcription factor and an ACT-like domain, has an rxp allele producing a truncated protein that confers resistance to pustule-causing Xanthomonas axonopodis pv. glycines. In soybean, bacterial pustules caused by Xanthomonas axonopodis pv. glycines lead to premature defoliation and decreased yield in warm, wet climates. In the USA, approximately 70 years ago, bacterial pustules were eliminated by introducing a recessive resistance allele, rxp, of the Rxp gene, representing the first example of successful soybean breeding for durable disease resistance in North America. In this study, we isolated this historical Rxp gene from resistant soybean varieties using positional cloning. The 1.06 Mb region where Rxp was reported to reside was narrowed down to an 11.1 kb region containing a single gene, Glyma.17g090500. The resistance allele, rxp, contains a T insertion. A complementation test of the Rxp allele in resistant plants confirmed the identification of the Rxp gene. The product of the susceptible wild-type allele, Rxp, is presumed to be a basic helix–loop–helix (bHLH) transcription factor with an aspartate kinase, chorismate mutase, and TyrA (ACT)–like domain. This gene was mainly expressed in extended leaves, and its homologs were identified to be distributed in angiosperms. A total of six alleles were obtained: four from spontaneous variation, including the wild-type and three mutant alleles that encoded truncated proteins, and two from ethyl methanesulfonate mutants, including an allele that encoded a truncated protein and a missense allele. By evaluating the resistance of these six alleles, we found that the loss of function of RXP decreased the bacterial pustule lesions. This study provides important insights into the soybean rxp allele, which confers durable resistance to bacterial pustules. [ABSTRACT FROM AUTHOR] |
| Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Header | DbId: egs DbLabel: Engineering Source An: 180646491 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: A single-nucleotide insertion in Rxp confers durable resistance to bacterial pustule in soybean. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Taguchi-Shiobara%2C+Fumio%22">Taguchi-Shiobara, Fumio</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> fstagu@naro.affrc.go.jp</i><br /><searchLink fieldCode="AR" term="%22Takahashi%2C+Koji%22">Takahashi, Koji</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yano%2C+Ryoichi%22">Yano, Ryoichi</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Suzuki%2C+Rintaro%22">Suzuki, Rintaro</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yokota%2C+Yuko%22">Yokota, Yuko</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yamazaki%2C+Toshimasa%22">Yamazaki, Toshimasa</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yamada%2C+Tetsuya%22">Yamada, Tetsuya</searchLink><relatesTo>1,4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sayama%2C+Takashi%22">Sayama, Takashi</searchLink><relatesTo>1,5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yamada%2C+Naohiro%22">Yamada, Naohiro</searchLink><relatesTo>1,6</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Oki%2C+Nobuhiko%22">Oki, Nobuhiko</searchLink><relatesTo>7</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Anai%2C+Toyoaki%22">Anai, Toyoaki</searchLink><relatesTo>8,9</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kaga%2C+Akito%22">Kaga, Akito</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ishimoto%2C+Masao%22">Ishimoto, Masao</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> ishimoto@naro.affrc.go.jp</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Theoretical+%26+Applied+Genetics%22">Theoretical & Applied Genetics</searchLink>. Nov2024, Vol. 137 Issue 11, p1-15. 15p. – Name: Abstract Label: Abstract Group: Ab Data: Key message: The soybean Rxp gene, encoding a bHLH transcription factor and an ACT-like domain, has an rxp allele producing a truncated protein that confers resistance to pustule-causing Xanthomonas axonopodis pv. glycines. In soybean, bacterial pustules caused by Xanthomonas axonopodis pv. glycines lead to premature defoliation and decreased yield in warm, wet climates. In the USA, approximately 70 years ago, bacterial pustules were eliminated by introducing a recessive resistance allele, rxp, of the Rxp gene, representing the first example of successful soybean breeding for durable disease resistance in North America. In this study, we isolated this historical Rxp gene from resistant soybean varieties using positional cloning. The 1.06 Mb region where Rxp was reported to reside was narrowed down to an 11.1 kb region containing a single gene, Glyma.17g090500. The resistance allele, rxp, contains a T insertion. A complementation test of the Rxp allele in resistant plants confirmed the identification of the Rxp gene. The product of the susceptible wild-type allele, Rxp, is presumed to be a basic helix–loop–helix (bHLH) transcription factor with an aspartate kinase, chorismate mutase, and TyrA (ACT)–like domain. This gene was mainly expressed in extended leaves, and its homologs were identified to be distributed in angiosperms. A total of six alleles were obtained: four from spontaneous variation, including the wild-type and three mutant alleles that encoded truncated proteins, and two from ethyl methanesulfonate mutants, including an allele that encoded a truncated protein and a missense allele. By evaluating the resistance of these six alleles, we found that the loss of function of RXP decreased the bacterial pustule lesions. This study provides important insights into the soybean rxp allele, which confers durable resistance to bacterial pustules. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=180646491 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00122-024-04743-5 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 15 StartPage: 1 Titles: – TitleFull: A single-nucleotide insertion in Rxp confers durable resistance to bacterial pustule in soybean. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Taguchi-Shiobara, Fumio – PersonEntity: Name: NameFull: Takahashi, Koji – PersonEntity: Name: NameFull: Yano, Ryoichi – PersonEntity: Name: NameFull: Suzuki, Rintaro – PersonEntity: Name: NameFull: Yokota, Yuko – PersonEntity: Name: NameFull: Yamazaki, Toshimasa – PersonEntity: Name: NameFull: Yamada, Tetsuya – PersonEntity: Name: NameFull: Sayama, Takashi – PersonEntity: Name: NameFull: Yamada, Naohiro – PersonEntity: Name: NameFull: Oki, Nobuhiko – PersonEntity: Name: NameFull: Anai, Toyoaki – PersonEntity: Name: NameFull: Kaga, Akito – PersonEntity: Name: NameFull: Ishimoto, Masao IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 11 Text: Nov2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 00405752 Numbering: – Type: volume Value: 137 – Type: issue Value: 11 Titles: – TitleFull: Theoretical & Applied Genetics Type: main |
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