View in EDS HTML Full Text PDF Full Text

Production of Fermented Solid Containing Lipases from Penicillium polonicum and Its Direct Use as Biocatalyst in the Synthesis of Ethyl Oleate.

Saved in:
Bibliographic Details
Title: Production of Fermented Solid Containing Lipases from Penicillium polonicum and Its Direct Use as Biocatalyst in the Synthesis of Ethyl Oleate.
Authors: Carvalho, Jéssyca Ketterine1,2 (AUTHOR) jessycakcarvalho@gmail.com, Zanella, Ricardo Antonio2 (AUTHOR), Piana, Pitágoras Augusto2 (AUTHOR), Rosado, Adriana Fiorini1 (AUTHOR), da Silva, Mairim Dahm2 (AUTHOR), Lucca, Rosemeire Aparecida da Silva de2 (AUTHOR), Fagundes-Klen, Marcia Regina2 (AUTHOR), da Silva, Edson Antônio2 (AUTHOR), Zanella, Karine3 (AUTHOR), Buzanello, Cleide Viviane2 (AUTHOR), Onofrio, Álvaro Barcellos4 (AUTHOR), Rodrigues, Maria Luiza Fernandes2 (AUTHOR)
Source: BioEnergy Research. Dec2024, Vol. 17 Issue 4, p2440-2449. 10p.
Subjects: Biosynthesis, Solid-state fermentation, Ethyl esters, Sunflower seeds, Catalyst synthesis, Heptane
Abstract: Enzymatic catalysts, such as lipases, have been extensively studied due to their promise as an alternative to chemical catalysts. They offer advantages like biodegradability (green biotechnology) and the potential for enzyme recycling (turnover), leading to reduced operational costs. The primary objective of this study was to produce lipase from the fungus Penicillium polonicum through solid-state fermentation, utilizing agro-industrial waste as substrate. The aim was to apply the obtained lipase as a biological catalyst in the synthesis of ethyl oleate ester. In the lipase production process, the filamentous fungus P. polonicum, along with sunflower seed cake (SSC) and rice husk (RH), served as substrate and support, respectively, for solid-state fermentation (SSF). Experiments involved varying proportions of both substrate and support (0%, 25%, 50%, 75%, and 100% (RH) and 100% (SSC)). Subsequently, the impact of glycerol as an inducer (1%, 3%, and 5%, with a 25/75% SSC/RH ratio) at SSF was investigated. The obtained results revealed a catalytic activity of 29.3 U g−1 under optimal conditions: 55% moisture, 25/75% SSC/RH, and at 27 °C during 96 h of fermentation. The lipase produced was employed as an enzymatic catalyst in studies involving the synthesis of ethyl oleate ester in n-heptane, utilizing experimental design 23. Variables such as temperature, enzymatic activity, and molar ratio (alcohol/acid) were modified. The best experimental conditions for the enzymatic synthesis of ethyl oleate ester were determined to be an alcohol/acid molar ratio of 6:1, a temperature of 37 °C, and an enzymatic activity of 60 U. This resulted in 100% conversion into ester within 5 h of reaction time. The outcomes demonstrated that lipase effectively catalyzed the synthesis of ethyl oleate, a biodiesel ester, with a high yield. [ABSTRACT FROM AUTHOR]
Copyright of BioEnergy Research is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Full text is not displayed to guests.
FullText Links:
  – Type: pdflink
Text:
  Availability: 1
Header DbId: egs
DbLabel: Engineering Source
An: 180934252
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Production of Fermented Solid Containing Lipases from Penicillium polonicum and Its Direct Use as Biocatalyst in the Synthesis of Ethyl Oleate.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Carvalho%2C+Jéssyca+Ketterine%22">Carvalho, Jéssyca Ketterine</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> jessycakcarvalho@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Zanella%2C+Ricardo+Antonio%22">Zanella, Ricardo Antonio</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Piana%2C+Pitágoras+Augusto%22">Piana, Pitágoras Augusto</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Rosado%2C+Adriana+Fiorini%22">Rosado, Adriana Fiorini</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22da+Silva%2C+Mairim+Dahm%22">da Silva, Mairim Dahm</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lucca%2C+Rosemeire+Aparecida+da+Silva+de%22">Lucca, Rosemeire Aparecida da Silva de</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Fagundes-Klen%2C+Marcia+Regina%22">Fagundes-Klen, Marcia Regina</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22da+Silva%2C+Edson+Antônio%22">da Silva, Edson Antônio</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zanella%2C+Karine%22">Zanella, Karine</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Buzanello%2C+Cleide+Viviane%22">Buzanello, Cleide Viviane</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Onofrio%2C+Álvaro+Barcellos%22">Onofrio, Álvaro Barcellos</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Rodrigues%2C+Maria+Luiza+Fernandes%22">Rodrigues, Maria Luiza Fernandes</searchLink><relatesTo>2</relatesTo> (AUTHOR)
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22BioEnergy+Research%22">BioEnergy Research</searchLink>. Dec2024, Vol. 17 Issue 4, p2440-2449. 10p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Biosynthesis%22">Biosynthesis</searchLink><br /><searchLink fieldCode="DE" term="%22Solid-state+fermentation%22">Solid-state fermentation</searchLink><br /><searchLink fieldCode="DE" term="%22Ethyl+esters%22">Ethyl esters</searchLink><br /><searchLink fieldCode="DE" term="%22Sunflower+seeds%22">Sunflower seeds</searchLink><br /><searchLink fieldCode="DE" term="%22Catalyst+synthesis%22">Catalyst synthesis</searchLink><br /><searchLink fieldCode="DE" term="%22Heptane%22">Heptane</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Enzymatic catalysts, such as lipases, have been extensively studied due to their promise as an alternative to chemical catalysts. They offer advantages like biodegradability (green biotechnology) and the potential for enzyme recycling (turnover), leading to reduced operational costs. The primary objective of this study was to produce lipase from the fungus Penicillium polonicum through solid-state fermentation, utilizing agro-industrial waste as substrate. The aim was to apply the obtained lipase as a biological catalyst in the synthesis of ethyl oleate ester. In the lipase production process, the filamentous fungus P. polonicum, along with sunflower seed cake (SSC) and rice husk (RH), served as substrate and support, respectively, for solid-state fermentation (SSF). Experiments involved varying proportions of both substrate and support (0%, 25%, 50%, 75%, and 100% (RH) and 100% (SSC)). Subsequently, the impact of glycerol as an inducer (1%, 3%, and 5%, with a 25/75% SSC/RH ratio) at SSF was investigated. The obtained results revealed a catalytic activity of 29.3 U g−1 under optimal conditions: 55% moisture, 25/75% SSC/RH, and at 27 °C during 96 h of fermentation. The lipase produced was employed as an enzymatic catalyst in studies involving the synthesis of ethyl oleate ester in n-heptane, utilizing experimental design 23. Variables such as temperature, enzymatic activity, and molar ratio (alcohol/acid) were modified. The best experimental conditions for the enzymatic synthesis of ethyl oleate ester were determined to be an alcohol/acid molar ratio of 6:1, a temperature of 37 °C, and an enzymatic activity of 60 U. This resulted in 100% conversion into ester within 5 h of reaction time. The outcomes demonstrated that lipase effectively catalyzed the synthesis of ethyl oleate, a biodiesel ester, with a high yield. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of BioEnergy Research is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=180934252
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1007/s12155-024-10772-1
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 10
        StartPage: 2440
    Subjects:
      – SubjectFull: Biosynthesis
        Type: general
      – SubjectFull: Solid-state fermentation
        Type: general
      – SubjectFull: Ethyl esters
        Type: general
      – SubjectFull: Sunflower seeds
        Type: general
      – SubjectFull: Catalyst synthesis
        Type: general
      – SubjectFull: Heptane
        Type: general
    Titles:
      – TitleFull: Production of Fermented Solid Containing Lipases from Penicillium polonicum and Its Direct Use as Biocatalyst in the Synthesis of Ethyl Oleate.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Carvalho, Jéssyca Ketterine
      – PersonEntity:
          Name:
            NameFull: Zanella, Ricardo Antonio
      – PersonEntity:
          Name:
            NameFull: Piana, Pitágoras Augusto
      – PersonEntity:
          Name:
            NameFull: Rosado, Adriana Fiorini
      – PersonEntity:
          Name:
            NameFull: da Silva, Mairim Dahm
      – PersonEntity:
          Name:
            NameFull: Lucca, Rosemeire Aparecida da Silva de
      – PersonEntity:
          Name:
            NameFull: Fagundes-Klen, Marcia Regina
      – PersonEntity:
          Name:
            NameFull: da Silva, Edson Antônio
      – PersonEntity:
          Name:
            NameFull: Zanella, Karine
      – PersonEntity:
          Name:
            NameFull: Buzanello, Cleide Viviane
      – PersonEntity:
          Name:
            NameFull: Onofrio, Álvaro Barcellos
      – PersonEntity:
          Name:
            NameFull: Rodrigues, Maria Luiza Fernandes
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 12
              Text: Dec2024
              Type: published
              Y: 2024
          Identifiers:
            – Type: issn-print
              Value: 19391234
          Numbering:
            – Type: volume
              Value: 17
            – Type: issue
              Value: 4
          Titles:
            – TitleFull: BioEnergy Research
              Type: main
ResultId 1