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Mueller matrix analysis of a biologically sourced engineered tissue construct as polarimetric phantom.

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Title: Mueller matrix analysis of a biologically sourced engineered tissue construct as polarimetric phantom.
Authors: Lin, Zixi1, Madnick, Samantha2,3, Burrow, Joshua A.1, Morgan, Jeffrey R.2,3, Toussaint Jr., Kimani C.1,4 kimani_toussaint@brown.edu
Source: Journal of Biomedical Optics. Oct2024, Vol. 29 Issue 10, p1-17. 17p.
Subjects: T-matrix, Transforming growth factors, Mueller calculus, Matrix decomposition, Tissue culture
Abstract: Significance: The polarimetric properties of biological tissues are often difficult to ascertain independent of their complex structural and organizational features. Conventional polarimetric tissue phantoms have well-characterized optical properties but are overly simplified. We demonstrate that an innovative, biologically sourced, engineered tissue construct better recapitulates the desired structural and polarimetric properties of native collagenous tissues, with the added benefit of potential tunability of the polarimetric response. We bridge the gap between nonbiological polarimetric phantoms and native tissues. Aim: We aim to evaluate a synthesized tissue construct for its effectiveness as a phantom that mimics the polarimetric properties in typical collagenous tissues. Approach: We use a fibroblast-derived, ring-shaped engineered tissue construct as an innovative tissue phantom for polarimetric imaging. We perform polarimetry measurements and subsequent analysis using the Mueller matrix decomposition and Mueller matrix transformation methods. Scalar polarimetric parameters of the engineered tissue are analyzed at different time points for both a control group and for those treated with the transforming growth factor ðTGFÞ-β1. Second-harmonic generation (SHG) imaging and three-dimensional collagen fiber organization analysis are also applied. Results: We identify linear retardance and circular depolarization as the parameters that are most sensitive to the tissue culture time and the addition of TGF-β1. Aside from a statistically significant increase over time, the behavior of linear retardance and circular depolarization indicates that the addition of TGF-β1 accelerates the growth of the engineered tissue, which is consistent with expectations. We also find through SHG images that collagen fiber organization becomes more aligned over time but is not susceptible to the addition of TGF-β1. Conclusions: The engineered tissue construct exhibits changes in polarimetric properties, especially linear retardance and circular depolarization, over culture time and under TGF-β1 treatments. This tissue construct has the potential to act as a controlled modular optical phantom for polarimetric-based methods. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Biomedical Optics is the property of SPIE - International Society of Optical Engineering and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
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PubType: Academic Journal
PubTypeId: academicJournal
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  Label: Title
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  Data: Mueller matrix analysis of a biologically sourced engineered tissue construct as polarimetric phantom.
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  Data: <searchLink fieldCode="AR" term="%22Lin%2C+Zixi%22">Lin, Zixi</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Madnick%2C+Samantha%22">Madnick, Samantha</searchLink><relatesTo>2,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Burrow%2C+Joshua+A%2E%22">Burrow, Joshua A.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Morgan%2C+Jeffrey+R%2E%22">Morgan, Jeffrey R.</searchLink><relatesTo>2,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Toussaint+Jr%2E%2C+Kimani+C%2E%22">Toussaint Jr., Kimani C.</searchLink><relatesTo>1,4</relatesTo><i> kimani_toussaint@brown.edu</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Biomedical+Optics%22">Journal of Biomedical Optics</searchLink>. Oct2024, Vol. 29 Issue 10, p1-17. 17p.
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  Data: <searchLink fieldCode="DE" term="%22T-matrix%22">T-matrix</searchLink><br /><searchLink fieldCode="DE" term="%22Transforming+growth+factors%22">Transforming growth factors</searchLink><br /><searchLink fieldCode="DE" term="%22Mueller+calculus%22">Mueller calculus</searchLink><br /><searchLink fieldCode="DE" term="%22Matrix+decomposition%22">Matrix decomposition</searchLink><br /><searchLink fieldCode="DE" term="%22Tissue+culture%22">Tissue culture</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Significance: The polarimetric properties of biological tissues are often difficult to ascertain independent of their complex structural and organizational features. Conventional polarimetric tissue phantoms have well-characterized optical properties but are overly simplified. We demonstrate that an innovative, biologically sourced, engineered tissue construct better recapitulates the desired structural and polarimetric properties of native collagenous tissues, with the added benefit of potential tunability of the polarimetric response. We bridge the gap between nonbiological polarimetric phantoms and native tissues. Aim: We aim to evaluate a synthesized tissue construct for its effectiveness as a phantom that mimics the polarimetric properties in typical collagenous tissues. Approach: We use a fibroblast-derived, ring-shaped engineered tissue construct as an innovative tissue phantom for polarimetric imaging. We perform polarimetry measurements and subsequent analysis using the Mueller matrix decomposition and Mueller matrix transformation methods. Scalar polarimetric parameters of the engineered tissue are analyzed at different time points for both a control group and for those treated with the transforming growth factor ðTGFÞ-β1. Second-harmonic generation (SHG) imaging and three-dimensional collagen fiber organization analysis are also applied. Results: We identify linear retardance and circular depolarization as the parameters that are most sensitive to the tissue culture time and the addition of TGF-β1. Aside from a statistically significant increase over time, the behavior of linear retardance and circular depolarization indicates that the addition of TGF-β1 accelerates the growth of the engineered tissue, which is consistent with expectations. We also find through SHG images that collagen fiber organization becomes more aligned over time but is not susceptible to the addition of TGF-β1. Conclusions: The engineered tissue construct exhibits changes in polarimetric properties, especially linear retardance and circular depolarization, over culture time and under TGF-β1 treatments. This tissue construct has the potential to act as a controlled modular optical phantom for polarimetric-based methods. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
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  Data: <i>Copyright of Journal of Biomedical Optics is the property of SPIE - International Society of Optical Engineering and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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        Value: 10.1117/1.JBO.29.10.106002
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        Text: English
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      – SubjectFull: Mueller calculus
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      – SubjectFull: Matrix decomposition
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      – SubjectFull: Tissue culture
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      – TitleFull: Mueller matrix analysis of a biologically sourced engineered tissue construct as polarimetric phantom.
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            NameFull: Lin, Zixi
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              M: 10
              Text: Oct2024
              Type: published
              Y: 2024
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