Investigating microstructural changes between in vivo and perfused ex vivo marmoset brains using oscillating gradient and b‐tensor encoded diffusion MRI at 9.4 T.
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| Title: | Investigating microstructural changes between in vivo and perfused ex vivo marmoset brains using oscillating gradient and b‐tensor encoded diffusion MRI at 9.4 T. |
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| Authors: | Santini, Tales1,2 (AUTHOR), Shim, Alyson1 (AUTHOR), Liou, Jr‐Jiun2 (AUTHOR), Rahman, Naila1 (AUTHOR), Varela‐Mattatall, Gabriel1 (AUTHOR), Budde, Matthew D.3 (AUTHOR), Inoue, Wataru1 (AUTHOR), Everling, Stefan1 (AUTHOR), Baron, Corey A.1 (AUTHOR) corey.baron@uwo.ca |
| Source: | Magnetic Resonance in Medicine. Feb2025, Vol. 93 Issue 2, p788-802. 15p. |
| Subjects: | Monte Carlo method, Diffusion magnetic resonance imaging, Extracellular fluid, Tissue fixation (Histology), Estimation theory |
| Abstract: | Purpose: To investigate microstructural alterations induced by perfusion fixation in brain tissues using advanced diffusion MRI techniques and estimate their potential impact on the application of ex vivo models to in vivo microstructure. Methods: We used oscillating gradient spin echo (OGSE) and b‐tensor encoding diffusion MRI to examine in vivo and ex vivo microstructural differences in the marmoset brain. OGSE was used to shorten effective diffusion times, whereas b‐tensor encoding allowed for the differentiation of isotropic and anisotropic kurtosis. Additionally, we performed Monte Carlo simulations to estimate the potential microstructural changes in the tissues. Results: We report large changes (˜50%–60%) in kurtosis frequency dispersion (OGSE) and in both anisotropic and isotropic kurtosis (b‐tensor encoding) after perfusion fixation. Structural MRI showed an average volume reduction of about 10%. Monte Carlo simulations indicated that these alterations could likely be attributed to extracellular fluid loss possibly combined with axon beading and increased dot compartment signal fraction. Little evidence was observed for reductions in axonal caliber. Conclusion: Our findings shed light on advanced MRI parameter changes that are induced by perfusion fixation and potential microstructural sources for these changes. This work also suggests that caution should be exercised when applying ex vivo models to infer in vivo tissue microstructure, as significant differences may arise. [ABSTRACT FROM AUTHOR] |
| Copyright of Magnetic Resonance in Medicine is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 181194751 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Investigating microstructural changes between in vivo and perfused ex vivo marmoset brains using oscillating gradient and b‐tensor encoded diffusion MRI at 9.4 T. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Santini%2C+Tales%22">Santini, Tales</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Shim%2C+Alyson%22">Shim, Alyson</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Liou%2C+Jr‐Jiun%22">Liou, Jr‐Jiun</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Rahman%2C+Naila%22">Rahman, Naila</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Varela‐Mattatall%2C+Gabriel%22">Varela‐Mattatall, Gabriel</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Budde%2C+Matthew+D%2E%22">Budde, Matthew D.</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Inoue%2C+Wataru%22">Inoue, Wataru</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Everling%2C+Stefan%22">Everling, Stefan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Baron%2C+Corey+A%2E%22">Baron, Corey A.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> corey.baron@uwo.ca</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Magnetic+Resonance+in+Medicine%22">Magnetic Resonance in Medicine</searchLink>. Feb2025, Vol. 93 Issue 2, p788-802. 15p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Monte+Carlo+method%22">Monte Carlo method</searchLink><br /><searchLink fieldCode="DE" term="%22Diffusion+magnetic+resonance+imaging%22">Diffusion magnetic resonance imaging</searchLink><br /><searchLink fieldCode="DE" term="%22Extracellular+fluid%22">Extracellular fluid</searchLink><br /><searchLink fieldCode="DE" term="%22Tissue+fixation+%28Histology%29%22">Tissue fixation (Histology)</searchLink><br /><searchLink fieldCode="DE" term="%22Estimation+theory%22">Estimation theory</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Purpose: To investigate microstructural alterations induced by perfusion fixation in brain tissues using advanced diffusion MRI techniques and estimate their potential impact on the application of ex vivo models to in vivo microstructure. Methods: We used oscillating gradient spin echo (OGSE) and b‐tensor encoding diffusion MRI to examine in vivo and ex vivo microstructural differences in the marmoset brain. OGSE was used to shorten effective diffusion times, whereas b‐tensor encoding allowed for the differentiation of isotropic and anisotropic kurtosis. Additionally, we performed Monte Carlo simulations to estimate the potential microstructural changes in the tissues. Results: We report large changes (˜50%–60%) in kurtosis frequency dispersion (OGSE) and in both anisotropic and isotropic kurtosis (b‐tensor encoding) after perfusion fixation. Structural MRI showed an average volume reduction of about 10%. Monte Carlo simulations indicated that these alterations could likely be attributed to extracellular fluid loss possibly combined with axon beading and increased dot compartment signal fraction. Little evidence was observed for reductions in axonal caliber. Conclusion: Our findings shed light on advanced MRI parameter changes that are induced by perfusion fixation and potential microstructural sources for these changes. This work also suggests that caution should be exercised when applying ex vivo models to infer in vivo tissue microstructure, as significant differences may arise. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Magnetic Resonance in Medicine is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/mrm.30298 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 15 StartPage: 788 Subjects: – SubjectFull: Monte Carlo method Type: general – SubjectFull: Diffusion magnetic resonance imaging Type: general – SubjectFull: Extracellular fluid Type: general – SubjectFull: Tissue fixation (Histology) Type: general – SubjectFull: Estimation theory Type: general Titles: – TitleFull: Investigating microstructural changes between in vivo and perfused ex vivo marmoset brains using oscillating gradient and b‐tensor encoded diffusion MRI at 9.4 T. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Santini, Tales – PersonEntity: Name: NameFull: Shim, Alyson – PersonEntity: Name: NameFull: Liou, Jr‐Jiun – PersonEntity: Name: NameFull: Rahman, Naila – PersonEntity: Name: NameFull: Varela‐Mattatall, Gabriel – PersonEntity: Name: NameFull: Budde, Matthew D. – PersonEntity: Name: NameFull: Inoue, Wataru – PersonEntity: Name: NameFull: Everling, Stefan – PersonEntity: Name: NameFull: Baron, Corey A. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 02 Text: Feb2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 07403194 Numbering: – Type: volume Value: 93 – Type: issue Value: 2 Titles: – TitleFull: Magnetic Resonance in Medicine Type: main |
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