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Hybrid Structures Based on Silver Nanoparticles and Polyelectrolytes for Sensitive Fluorescent Solid-Phase Immunoassay.

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Title: Hybrid Structures Based on Silver Nanoparticles and Polyelectrolytes for Sensitive Fluorescent Solid-Phase Immunoassay.
Authors: Kulakovich, O. S.1 (AUTHOR) o.kulakovich@ifanbel.bas-net.by, Ramanenka, A. A.1 (AUTHOR), Koktysh, I. V.2 (AUTHOR), Melnikova, Ya. I.2 (AUTHOR), Stsiapura, V. I.2,3 (AUTHOR), Gaponenko, S. V.1 (AUTHOR), Maskevich, S. A.2 (AUTHOR)
Source: Journal of Applied Spectroscopy. Nov2024, Vol. 91 Issue 5, p1025-1031. 7p.
Subjects: Silver nanoparticles, Surface plates, Fluorescein isothiocyanate, Nanoparticles, Immunoassay
Abstract: The effect of surface modification of polystyrene plates for immunoassay with silver nanoparticles and polyelectrolytes on the fluorescent signal of adsorbed antibodies with fluorescent labels IgG-FITC, IgG-AF488 and IgG-AF647 was studied. The resulting solid phase was characterized by a sorption capacity increased by 30–50% as compared to high-binding polystyrene (Greiner, Austria) and the ability to enhance the fluorescent signals of the labels due to a plasmonic effect, which together increased the fluorescence intensity by 3–15 times, depending on the type of plate surface, the nature of the polyelectrolyte, and the optical and physicochemical properties of the labeled antibodies. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Applied Spectroscopy is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 181201970
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PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Hybrid Structures Based on Silver Nanoparticles and Polyelectrolytes for Sensitive Fluorescent Solid-Phase Immunoassay.
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  Data: The effect of surface modification of polystyrene plates for immunoassay with silver nanoparticles and polyelectrolytes on the fluorescent signal of adsorbed antibodies with fluorescent labels IgG-FITC, IgG-AF488 and IgG-AF647 was studied. The resulting solid phase was characterized by a sorption capacity increased by 30–50% as compared to high-binding polystyrene (Greiner, Austria) and the ability to enhance the fluorescent signals of the labels due to a plasmonic effect, which together increased the fluorescence intensity by 3–15 times, depending on the type of plate surface, the nature of the polyelectrolyte, and the optical and physicochemical properties of the labeled antibodies. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Journal of Applied Spectroscopy is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – SubjectFull: Fluorescein isothiocyanate
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              Text: Nov2024
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